FOXO4 Peptide Lyophilized Powder – Full Compliance English Description (No Sensitive Words)
This product is FOXO4 targeted research-grade lyophilized polypeptide powder with the CAS registry number 271597-12-7. It is produced via mature Fmoc solid-phase peptide synthesis technology, followed by multi-cycle reversed-phase high-performance liquid chromatography purification and standard vacuum freeze-drying manufacturing process. Polypeptides are small organic molecules composed of two or more amino acid units connected by peptide bonds through dehydration condensation. This FOXO4 peptide features accurately arranged amino acid sequences, stable molecular spatial conformation, and molecular weight fully consistent with standard mass spectrometry identification data.
Compared with macromolecular proteins that feature complex structures and unstable batch reproducibility, this FOXO4 peptide possesses clear molecular composition, definite target binding sites, stable bioactivity under in vitro experimental conditions, simple dissolution and dilution operation, as well as excellent permeability and high controllability for cell-level and molecular-level research applications. The entire manufacturing process strictly follows ISO9001 quality management system and standard dust-free laminar flow workshop operating specifications. All finished products are manufactured in accordance with high-purity biochemical raw material standards referenced by USP and EP guidelines, achieving full-process traceability covering raw material feeding, directional peptide chain synthesis, side-chain deprotection and molecular cleavage, chromatographic purification, impurity filtration, vacuum freeze-drying dehydration and quantitative standardized subpackaging.
Multiple quality control procedures are implemented throughout production to effectively avoid exogenous contamination, thermal interference and microbial growth risks. This FOXO4 peptide is mainly supplied to university research laboratories, life science research institutions, biochemical experimental platforms, cell biology research teams, cosmetic ingredient development enterprises and functional material research organizations, providing standardized high-purity research raw materials and customized peptide synthesis and modification services.
The standard specification is 5mg per neutral borosilicate glass vial. The product appears as uniform, loose, white sponge-like lyophilized powder without caking, color spots, visible particles or spontaneous deliquescence. Residual moisture is precisely controlled between 1% and 3%, which effectively preserves the complete spatial structure of FOXO4 peptide molecules. It prevents molecular hydrolysis, oxidative denaturation and peptide chain fracture caused by long-term exposure to ambient temperature and humid environments, ensuring stable biological binding activity for experimental use.
As a preferred stable storage form for polypeptide research materials in global academic research and formula development fields, lyophilized powder maintains stable performance for up to 24 months under sealed low-temperature storage. Different from liquid peptide solutions that are vulnerable to deterioration during transit, this freeze-dried product greatly reduces quality attenuation and activity loss during long-term storage and cross-border logistics, effectively lowering inventory risk for research purchasers.
2. Complete Physicochemical Index & Standard Specification Parameters
2.1 Basic Morphology & Physical Properties
Appearance Form: Loose and porous white lyophilized powder contained in neutral borosilicate glass vials. It dissolves rapidly and completely within 30 seconds in sterile deionized water or PBS buffer solution, producing a clear, colorless and transparent solution without sediment, floccules or layered precipitation. No visible particulate impurities can be observed under normal visual inspection.
Hygroscopic Performance: FOXO4 peptide powder presents mild hygroscopicity under regular atmospheric conditions. Once the vial seal is opened, weighing, pipetting and sample splitting should be completed promptly to prevent moisture absorption, deliquescence and structural changes. Each vial adopts a dual-sealing structure with rubber stopper and aluminum crimp cap, combined with vacuum negative pressure sealing technology to isolate external air and moisture, maintaining stable internal powder condition.
Solubility Characteristics: The peptide is fully soluble in purified water, weakly acidic solutions and weakly alkaline buffer systems commonly used in biochemical laboratories. It shows extremely low solubility in conventional organic solvents including methanol, ethanol, isopropanol and acetone, and does not disperse in oil-based systems. This solubility characteristic perfectly adapts to standard solution preparation for in vitro cell experiments, molecular binding tests and formula development projects.
2.2 Purity Testing & Quality Control Benchmark
The core FOXO4 peptide component reaches a minimum purity of 99%. Reversed-phase high-performance liquid chromatography (RP-HPLC) main peak area normalization method is applied for purity verification. For each production batch, the main peak proportion remains above 99.0%, single impurity peak below 0.5%, and total impurity content less than 1.0%. All indicators meet high-purity biochemical raw material impurity standards referenced by USP and EP guidelines, eliminating synthesis by-product interference for downstream experimental research.
Each batch is accompanied by professional test documents including HPLC chromatogram reports and MS mass spectrometry identification certificates. Mass spectrometry verification confirms that the actual molecular weight deviates within ±1Da from the theoretical molecular weight, proving accurate amino acid sequence assembly without missing fragments, truncated segments or mis-sequence defects. Targeted oxidation impurity screening is added for oxidation-prone amino acid sites, strictly controlling oxidized derivatives and ensuring excellent batch consistency and experimental repeatability.
2.3 Diversified Packaging Specification System
Standard packaging: 5mg per neutral borosilicate glass vial. The vial features excellent low-temperature resistance and acid-base stability, suitable for long-term refrigerated storage at -20℃ without cracking or substance precipitation. The inner stopper and outer aluminum crimp cap provide stable sealing performance for regular laboratory operation.
Custom specifications are available, including 1mg, 2mg, 5mg, 10mg, 20mg, 50mg and 100mg per vial. Large bulk packaging of 1g, 5g and 10g with vacuum aluminum foil sealing is supported for industrial research use. All subpackaging procedures are completed in Class A laminar flow environment inside ten-thousand-level dust-free workshops to minimize contamination risk. Each vial is marked with exclusive serial number, batch code, production date, validity information, storage guidelines and traceable QR code for full production record inquiry.
2.4 Optional Quality Grades for Different Research Scenarios
Research Grade: Complies with strict biochemical raw material quality standards with low thermal interference and controlled microbial count, suitable for biological research experiments and in vitro mechanism exploration.
USP Reference Grade: Follows USP polypeptide raw material inspection standards for impurity limits, residual solvents, heavy metal content and ignition residues, meeting procurement requirements of North American research institutions and biological R&D enterprises.
EP Reference Grade: Complies with European Pharmacopoeia raw material management specifications, with strict control over synthesis solvent residues, suitable for EU regional biological research and project filing.
2.5 Residual Substance & Heavy Metal Control
Strict control is implemented for heavy metal elements including Pb, Cd, Hg, As and Cu, with single element content below 10ppm. The total ignition residue is ≤0.1%, and trifluoroacetic acid residual content is less than 0.1%. All organic solvent residues generated during synthesis are removed through rotary evaporation, dialysis desalting and repeated freeze-drying purification, ensuring no exogenous chemical interference for cell experiments and formula research.
3. Full Production Process Technical Interpretation
Step 1: Directional Solid-phase Peptide Synthesis
Classic Fmoc solid-phase synthesis technology is adopted using polystyrene resin as the fixed carrier. According to the standard FOXO4 amino acid sequence, cyclic condensation, deprotection and washing procedures are performed sequentially from C-terminal to N-terminal. Real-time coupling efficiency monitoring ensures full reaction completion and reduces mismatched amino acid impurities. Solid-phase synthesis delivers fewer side reactions, higher sequence accuracy and better purification efficiency, ideal for stable batch production of fixed-sequence peptides.
Step 2: Resin Cleavage & Crude Peptide Separation
After sequence assembly completion, matched cleavage reagent separates the complete peptide chain from resin carriers and removes side-chain protective groups. The cleavage solution is dropped into pre-cooled ice water for crude peptide precipitation, followed by high-speed refrigerated centrifugation and low-temperature ether washing to remove residual reagents and derivatives. Vacuum pre-drying is applied to obtain purified crude peptide powder and eliminate most macromolecular impurities.
Step 3: Gradient HPLC Purification & Desalination
Crude peptide powder is dissolved and separated via preparative reversed-phase HPLC with gradient elution. Target peptide main peaks are manually collected and merged, followed by ultrafiltration desalination to remove salt ions and mobile phase residues. The process stably elevates peptide purity above 99% and intercepts macromolecular impurities to optimize finished product purity consistency.
Step 4: Fine Filtration & Quantitative Filling
Purified peptide solution is filtered through 0.22μm filter membrane to remove fine particles and contaminants. The processed solution is precisely filled into pre-sterilized glass vials under Class A laminar flow environment in dust-free workshop to guarantee filling uniformity and external pollution prevention before freeze-drying.
Step 5: Program-controlled Vacuum Freeze-drying & Sealing
Filled vials undergo standardized three-stage freeze-drying: pre-freezing below -45℃, primary sublimation for free water removal, and secondary desorption for bound water elimination. Final residual moisture is stabilized within 3%. Fully sealed aluminum crimp caps are applied immediately after drying to isolate air and moisture, locking complete molecular stability.
Step 6: Batch Inspection, Coding & Warehousing
Each production batch undergoes full-item inspection including appearance check, HPLC purity test, MS molecular weight verification, microbial inspection, moisture detection and heavy metal screening. Qualified products are labeled with batch traceability codes and stored in -20℃ constant temperature dark environment for stable preservation before delivery.
4. Compliant Application Fields & Research Scenarios
Important Usage Statement: This FOXO4 peptide lyophilized powder is exclusively used as in vitro laboratory research material and formula development raw material. It is limited to scientific experiments, cell culture and molecular mechanism research. All usage is restricted to laboratory non-clinical research purposes only.
Scenario 1: Basic Life Science Laboratory Research
FOXO4 peptide can be used as target ligand and pathway regulator for cell target binding experiments, protein interaction tests and fluorescence tracing analysis. It supports academic research on gene regulation, cell proliferation, apoptosis, oxidative stress and cell aging signaling pathways. High batch stability ensures repeatable experimental data and minimizes impurity interference.
It serves as classic experimental material for constructing in vitro senescent cell models, assisting in screening functional active substances and verifying molecular mechanisms. The lyophilized form allows flexible solution preparation and multi-concentration gradient experimental design.
It is also applicable to in vitro immune cell induction and functional regulation research for T cells, macrophages and dendritic cells, supporting immune response, anti-inflammation and tumor microenvironment research with stable and low-interference performance.
Scenario 2: Biochemical R&D and Molecular Activity Research
Research institutions apply FOXO4 peptide for in vitro cellular activity screening and target interaction verification, helping evaluate molecular binding efficiency and research value of candidate substances. High-purity raw materials provide accurate concentration threshold data and avoid impurity-caused experimental deviation.
Modified FOXO4 peptide can be used as carrier framework material for targeted delivery system research, coupled with small molecules, nucleic acid fragments and nano-particles for targeted recognition formulation development. Its easy-dissolving property supports precise solution proportioning for laboratory formulation exploration.
Custom FOXO4 peptide standard samples and impurity references can be synthesized for laboratory testing, supporting content detection and methodological verification of self-developed research samples.
Scenario 3: Serum-free Cell Culture Medium Optimization
Replacing partial serum growth factor components, FOXO4 functional peptide optimizes serum-free culture systems to avoid traditional serum disadvantages including batch difference, unknown contamination risks and unstable quality. It improves stem cell adhesion and maintains undifferentiated characteristics during in vitro amplification.
It optimizes in vitro activation and proliferation of immune cell lines such as CAR-T and NK, improving cell activity and batch stability. It also standardizes tumor cell line culture by regulating cell growth status and reducing serum cytokine interference for more stable in vitro research models.
Scenario 4: Cosmetic Functional Ingredient Formula Development
As a high-quality polypeptide raw material for cosmetic formula research, FOXO4 lyophilized powder can be dissolved and added into essence, lotion, cream and mask formula systems for in vitro skin model research. It assists in studying keratin metabolism, skin barrier maintenance, antioxidant performance and soothing effects in cosmetic ingredient development.
Provided in pure powder form without extra solvents or preservatives, the material allows R&D teams to independently design formula systems with flexible auxiliary material compatibility. Complete qualification documents support cosmetic raw material filing and laboratory formula testing.
Scenario 5: Functional Raw Material Formula Research
FOXO4 peptide can be used for functional ingredient research and formula development of dietary supplementary materials. With strictly controlled heavy metal and microbial indicators, it supports laboratory formula testing, powder blending and liquid ingredient research for functional material development.
Scenario 6: Biological Detection Kit Raw Material Application
FOXO4 peptide can be used as coating antigen, positive control and calibration material for ELISA and immunochromatographic detection kits, improving detection specificity and sensitivity. Its high stability enables long-term laboratory storage and batch production of detection reagents.
5. Storage, Shelf Life & Logistics Instructions
Long-term Storage (6–24 months): Store unopened vials at -20℃ in dark and dry environment with humidity below 60%. Avoid frequent temperature fluctuation. Ultra-long storage can be maintained at -80℃ for extended stability.
Temporary Storage (1–3 months): Store at 2–8℃ away from light; room-temperature placement for long periods is not allowed.
After Opening: Dissolve and subpackage quickly, store liquid solution at -20℃ and use within 1 month. Avoid repeated freeze-thaw cycles to prevent molecular activity decline.
Shelf Life: 24 months for unopened products under standard -20℃ sealed dark storage.
Logistics: Products are packed with dry ice cold chain for domestic and international shipping to ensure low-temperature transportation stability and prevent activity loss during transit.
6. Custom Service & Cooperation Support
Custom peptide sequence synthesis, chemical modification, purity adjustment and personalized packaging and label services are available. Complete COA, HPLC and MS test reports are provided for each batch. ISO-standard production documents support enterprise research filing and cross-border trade.
7. Product Advantages
Strict full-process quality control, stable batch consistency, professional customization capability, complete document support and standardized cold chain packaging ensure reliable and repeatable research-grade raw material supply for global scientific and formula development users.
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