5- Amino-1MQ is the iodide form of 5-amino-1-methylquinolinium and is widely used as a small-molecule research compound for studying NNMT activity. It is not a peptide. NNMT is an enzyme involved in nicotinamide metabolism and cellular methyl-donor balance. In laboratory models, 5-Amino-1MQ is used to investigate NNMT-related metabolic pathways, enzyme activity, and cellular energy regulation.

Because NNMT links nicotinamide metabolism with cellular methyl-donor balance, 5-Amino-1MQ is a useful chemical probe for studying NAD+-related metabolism, SAM availability, adipocyte biology, lipid synthesis, cellular differentiation, and metabolic signaling. Published studies include enzyme assays, cultured adipocytes and muscle-cell systems, and animal models.

The compound as a selective NNMT research inhibitor and analytical or discovery reagent. Statements about body weight, glucose control, or other outcomes must remain clearly identified as preclinical research observations .

Research Mechanism and Scientific Rationale

5-Amino-1MQ inhibits NNMT activity, reducing conversion of nicotinamide to 1-methylnicotinamide. In experimental systems, NNMT inhibition has been associated with altered intracellular NAD+ and SAM pools and with downstream changes in lipid metabolism and cellular energy regulation.

Its methylquinolinium scaffold combines target activity with membrane permeability reported in the medicinal-chemistry literature. As with any chemical probe, selectivity and effective concentration should be validated in the specific assay system, and results should be interpreted with appropriate vehicle and target-engagement controls.

Importance in Biological and Chemical Research

5-Amino-1MQ enables direct experimental interrogation of NNMT, an enzyme positioned at the intersection of nicotinamide turnover, methyl metabolism, adipocyte function, and cellular energetics. It is valuable for mechanism-of-action work, pathway validation, and development of new NNMT-focused chemical series.

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• Key Research Applications

  NNMT Enzyme and Target-Engagement Assays

  Suitable for biochemical inhibition studies, potency evaluation, and measurement of NNMT reaction products.

  NAD+ and Methyl-Donor Metabolism Research

  Used to examine relationships among nicotinamide, NAD+-associated pathways, SAM, and methylation-related cellular metabolism.

  Adipocyte and Lipid-Metabolism Studies

  Supports cell-based research on adipocyte differentiation, lipid accumulation, lipogenesis, and metabolic signaling.

  Muscle-Cell and Regenerative Biology Research

  Applied in experimental models investigating myoblast differentiation, muscle stem-cell activity, and age-associated cellular changes.

  Medicinal Chemistry and Bioanalytical Method Development

  Useful as a reference inhibitor for analog screening, LC-MS/MS assay development, exposure analysis, and structure-activity studies.

Why Choose Our 5-Amino-1MQ (5-Amino-1-methylquinolinium Iodide)?

· Defined iodide salt with verified molecular identity and target-focused positioning.

· Suitable for enzyme, cell-based, metabolic, and analytical research workflows.

· Lot-specific HPLC and MS documentation available according to the ordered grade.

· Controlled impurity profile and packaging options for research-scale requirements.

· Technical support for storage, handling, analytical methods, and bulk inquiries.