Product Overview
Scheffoleoside A (CAS 160669-23-8) is a high‑purity triterpenoid saponin reference standard featuring asiatic acid as the aglycone with a trisaccharide chain attached at the 28‑position. The molecular formula is C₄₈H₇₈O₁₉ with a molecular weight of 935.14 g/mol. The sugar chain consists of three monosaccharide units arranged in a specific sequence: rhamnose-(1→4)-glucose-(1→6)-glucose. This compound is one of the major bioactive saponins isolated from the medicinal plant Centella asiatica (Gotu Kola), a well‑known herb in traditional medicine systems including Ayurveda and Traditional Chinese Medicine, widely used for wound healing, skin rejuvenation, and cognitive enhancement. As a research‑grade reference standard, it is essential for the identification and quantification of triterpenoid saponins in plant extracts and herbal products using HPLC‑DAD and UPLC‑MS/MS methods.
Synthetic Process / Isolation Method
This compound is typically isolated from the aerial parts of Centella asiatica through a series of extraction and chromatographic purification steps:
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Extraction: Dried plant material is extracted with aqueous ethanol or methanol under reflux.
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Partitioning: The crude extract is subjected to liquid‑liquid partitioning with n‑butanol to concentrate the saponin fraction.
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Column Chromatography: The enriched fraction undergoes repeated column chromatography using silica gel, macroporous resin, or Sephadex LH‑20, eluting with gradient solvent systems.
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Preparative HPLC: Final purification is achieved using preparative HPLC with a C18 reversed‑phase column.
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Recrystallization: The purified compound is recrystallized to achieve ≥95% purity.
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Quality Confirmation: The final product is rigorously characterized by HPLC‑PDA, ¹H‑NMR, ¹³C‑NMR, and high‑resolution mass spectrometry.
Mechanism of Action
As a triterpenoid saponin, scheffoleoside A exerts its biological activities through multiple mechanisms:
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Wound Healing: Promotes collagen synthesis and fibroblast proliferation, accelerating the wound healing process.
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Anti‑inflammatory Activity: Modulates inflammatory signaling pathways, reducing the production of pro‑inflammatory cytokines.
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Antioxidant Activity: Scavenges free radicals and reduces oxidative stress, protecting cells from damage.
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Neuroprotective Effects: Protects neuronal cells from oxidative damage and may have therapeutic potential in neurodegenerative diseases.
Quality Specifications
Our Scheffoleoside A is manufactured under strict quality control conditions: - Purity: ≥95.0% determined by HPLC - Identity confirmed by NMR and mass spectrometry - Water content: ≤1.0% - Heavy metals: ≤20 ppm - Each batch accompanied by comprehensive Certificate of Analysis (COA) - Stable for 24 months when stored at 2‑8°C in sealed containers protected from light and moisture
Packaging & Shipping
We offer flexible packaging options to meet diverse customer needs: - Research quantities: 2mg, 5mg, 10mg amber glass vials with PTFE‑lined caps, purged with inert gas - Bulk quantities: Available upon request - Custom packaging available upon request - Shipped at ambient temperature for routine orders - Cold chain shipping available upon request for temperature‑sensitive orders - International shipping available with proper documentation
Jinan Topfull Chemical Co., Ltd. is an international company rooted in China, specializing in the export of chemical products.
Our company is committed to making every effort to provide ideal chemical products to potential customers worldwide, including but not limited to: organic/inorganic reagents, biological reagents, plant extracts, pharmaceutical intermediates, active pharmaceutical ingredients (APIs), reference standards, reference substances, peptides, and more. Meanwhile, we have established long-term and stable cooperative relationships with university laboratories and off-campus research institutions, enabling us to offer tailored product services based on customers' actual needs.
We currently support customized services for most product categories, with supply quantities ranging from milligram (mg), gram (g) to kilogram (kg) levels, as well as wholesale. Product purity covers the full spectrum from 90% to 99.9%, providing customers with one-stop convenient services.
To date, our products have been exported to developed countries such as the United States, Australia, Canada, Mexico, as well as EU member states including France, Germany, and many other countries and regions such as Nigeria, Ghana, Southeast Asia, and other parts of Asia. Our product quality and services have consistently received high praise.
Looking ahead, our company will continue to serve as a bridge connecting China and the world, delivering high-quality products and services to global customers.
