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Home > Encyclopedia > Fluorescein isothiocyanate

Fluorescein isothiocyanate

Fluorescein isothiocyanate structure

Fluorescein isothiocyanate 

structure
  • CAS No:

    27072-45-3

  • Formula:

    C21H11NO5S

  • Chemical Name:

    Fluorescein isothiocyanate

  • Synonyms:

    Spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one,3′,6′-dihydroxy-5(or 6)-isothiocyanato-;Fluorescein,isothiocyanato-;3′,6′-Dihydroxy-5(or 6)-isothiocyanatospiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one;FITC;Fluorescein isothiocyanate;71: PN: WO2016072662 SEQID: 71 claimed sequence;28325-37-3;29792-10-7;64937-10-6;15042-94-1

  • Categories:

    Active Pharmaceutical Ingredients  >  Diagnostic Agents

Description

5(6)-FITC (Fluorescein isothiocyanate) is an amine-reactive derivative of fluorescein dye that has wide-ranging applications as a label for antibodies and other probes, for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA.

Fluorescein isothiocyanate Basic Attributes

389.38

389.035797

222-042-0

29321900

Characteristics

120.44000

4.00

powder

1.5±0.1 g/cm3

109-111 °C

708.6±60.0 °C at 760 mmHg

382.4±32.9 °C

1.754

2-8°C

Safety Information

NONH for all modes of transport

3

42/43-36/37/38-20/21/22-42

22-36-26-24/25-45-36/37

Xn

P261-P342 + P311

H334

Fluorescein isothiocyanate Use and Manufacturing

This product is a biochemical reagent as well as a medical diagnostic drug. It is mainly used as a fluorescent dye in fluorescent antibody technology. It can bind to various antibody proteins. The combined antibody does not lose the specificity of binding to a certain antigen, and it is in alkaline solution. It has strong green fluorescence. Used in medicine, agronomy and animal husbandry, it can quickly diagnose diseases caused by bacteria, viruses and parasites.

Material

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