5(6)-CFDA
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5(6)-CFDA
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CAS No:
124387-19-5
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Formula:
C50H32O18
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Chemical Name:
5(6)-CFDA
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Synonyms:
5(6)-CARBOXYFLUORESCEIN DIACETATE;5(6)-CFDA;5(6)-CARBOXY-DI-O-ACETYLFLUORESCEIN;5(6)-FAM DIACETATE;5-(AND-6)-CARBOXYFLUORESCEIN DIACETATE;5(6)-FAM DA;Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar-carboxylicacid, 3',6'-bis(acetyloxy)-3-oxo-;5(6)-CFDA [5(6)-Carboxyfluorescein diacetate]
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CAS No:
Description
5(6)-CFDA is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activity, which is require to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent product.
5(6)-Carboxyfluorescein diacetate (CFDA) is an amine reactive fluorescent probe used to stain cells with intact membranes for analysis in applications such as flow cytometry or fluorescence microscopy.
5(6)-CFDA Use and Manufacturing
CFDA is a popular, sensitive, fluorescent substrate for measuring esterase activity in live cells. It is often used in cell viability assays and "Live-Dead" assay systems, especially automated cell viability assays. The cleavage product produced upon enzy
5(6)-CFDA; 5(6)-Carboxyfluorescein Diacetate is a cell permeant, amine-reactive probe.