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Home > Encyclopedia > 3,3′,5,5′-Tetramethylbenzidine dihydrochloride

3,3′,5,5′-Tetramethylbenzidine dihydrochloride

3,3′,5,5′-Tetramethylbenzidine dihydrochloride structure

3,3′,5,5′-Tetramethylbenzidine dihydrochloride 

structure
  • CAS No:

    64285-73-0

  • Formula:

    C16H20N2.2ClH

  • Chemical Name:

    3,3′,5,5′-Tetramethylbenzidine dihydrochloride

  • Synonyms:

    [1,1′-Biphenyl]-4,4′-diamine,3,3′,5,5′-tetramethyl-,hydrochloride (1:2);[1,1′-Biphenyl]-4,4′-diamine,3,3′,5,5′-tetramethyl-,dihydrochloride;3,3′,5,5′-Tetramethylbenzidine dihydrochloride;TMBZ-HCl;TMB

  • Categories:

    Biochemical Engineering  >  Biochemical Reagents

Description

3,3’,5,5’-Tetramethylbenzidine(TMB) is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA).

3,3′,5,5′-Tetramethylbenzidine dihydrochloride Basic Attributes

313.26500

312.11600

264-769-6

DTXSID6070067

29215990

Characteristics

52.04000

6.51800

White to pale yellow powder.

>300ºC

368.6ºC at 760 mmHg

210.8ºC

2-8ºC

Safety Information

NONH for all modes of transport

3

R40

S22-S24/25

Xn

Stable under normal temperatures and pressures.

P201, P202, P261, P264, P270, P271, P280, P281, P301+P312, P302+P352, P304+P312, P304+P340, P308+P313, P312, P322, P330, P363, P405, P501

H302

|Warning|H302 (100%): Harmful if swallowed [Warning Acute toxicity, oral]|P201, P202, P261, P264, P270, P271, P280, P281, P301+P312, P302+P352, P304+P312, P304+P340, P308+P313, P312, P322, P330, P363, P405, and P501|The GHS information provided by 1 company from 1 notification to the ECHA C&L Inventory.

3,3′,5,5′-Tetramethylbenzidine dihydrochloride Use and Manufacturing

Uses

A peroxidase substrate suitable for use in ELISA procedures.

[1,1'-Biphenyl]-4,4'-diamine, 3,3',5,5'-tetramethyl-, hydrochloride (1:2): ACTIVE|PMN - indicates a commenced PMN (Pre-Manufacture Notices) substance.

Computed Properties

Molecular Weight:313.3
Hydrogen Bond Donor Count:4
Hydrogen Bond Acceptor Count:2
Rotatable Bond Count:1
Exact Mass:312.1160041
Monoisotopic Mass:312.1160041
Topological Polar Surface Area:52
Heavy Atom Count:20
Complexity:226
Covalently-Bonded Unit Count:3
Compound Is Canonicalized:Yes

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