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Propidium iodide

Propidium iodide structure

CAS No:
25535-16-4
Formula:
C27H34IN4+
Chemical Name:
Propidium iodide
Synonyms:
3,8-DIAMINO-5-(3-DIETHYL-AMINOPROPYL)-6-PHENYLPHENANTHRIDIUM IODIDE METHIODIDE;3,8-DIAMINO-5-(3-[DIETHYLMETHYLAMMONIO])-6-PHENYLPHENAN-THRIDINIUM DIIODIDE;3,8-DIAMINO-5-[3-(DIETHYLMETHYLAMMONIO)PROPYL]-6-PHENYLPHENANTHRIDINIUM;3,8-DIAMINO-5-[3-(DIETHYLMETHYLAMMONIO)PROPYL]-6-PHENYLPHENANTHRIDINIUM DIIODIDE;3,8-diamino-5-(3-(diethylmethylammonio)propyl)-6-phenyl-phenanthridiniudii;3,8-diamino-5-(3-(diethylmethylammonio)propyl)-6-phenylphenanthridiniumdiiod;3,8-diamino-5-(3-diethylaminopropyl)-6-phenylphenanthridiniumiodidemethiodi;PROPIDIUM IODIDE
Categories:
Pharmaceutical Intermediates > Bulk Drug Intermediates

Description

Propidium iodide is an organic iodide salt. It contains a propidium.|Propidium Iodide is a fluorescent nucleic acid dye which binds only to double-stranded nucleic acids. It has a molecular weight of 668.4, an absorbance maximum of 535nm, and an emission maximum of 617 nm. It is commonly used to determine the DNA content of a cell or to discriminate viable from non-viable cells.|Quaternary ammonium analog of ethidium; an intercalating dye with a specific affinity to certain forms of DNA and, used as diiodide, to separate them in density gradients; also forms fluorescent complexes with cholinesterase which it inhibits.

Propidium iodide is a dark red to reddish-brown liquid that is soluble in a variety of organic solvents. It is light-sensitive and may decompose upon prolonged exposure to light, leading to the formation of iodine. For optimal stability, it should be stored in a cool, dark place under an inert atmosphere.

ChEBI: Propidium iodide is an organic iodide salt. It contains a propidium.

Extinction coefficient(493 nm): 5900 M-1cm-1;(287 nm): 55,700 M-1cm-1;

It crystallises as red crystals from H2O containing a little KI. It fluoresces strongly with nucleic acids. [Watkins J Chem Soc 3064 1952, Beilstein 22 III/IV 5519.] TOXIC.

Propidium iodide Basic Attributes

Molecular Weight：

541.5

Exact Mass：

668.08729

BRN：

3843838

EC Number：

247-081-0

UNII：

TP416O228T

NCI Thesaurus Code：

C72903

Color/Form：

Dark red

HScode：

29339900

Characteristics

λmax：

493 nm, 535 nm

Density：

1.5072 (estimate)

Melting Point：

220-225 °C (dec.) (lit.)

Water Solubility：

Soluble in water at 1mg/ml

Critical Temperature & Pressure：

2-8°C

Safety Information

wgk germany：

hazard class code：

Xi,Xn

safety instructions：

26-36-22-36/37

RTECS Number：

SF7949600

Storage features：

+4°C

Hazard Statements：

36/37/38-68-67-48/20/22-40-36/38-22-20-63

GHS Classification：

|Warning|H315 (100%): Causes skin irritation [Warning Skin corrosion/irritation]|P201, P202, P261, P264, P271, P280, P281, P302+P352, P304+P340, P305+P351+P338, P308+P313, P312, P321, P332+P313, P337+P313, P362, P403+P233, P405, and P501|Aggregated GHS information provided by 162 companies from 6 notifications to the ECHA C&L Inventory. Each notification may be associated with multiple companies.

Drug Information

MeSH：

Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant and Hackh's Chemical Dictionary, 5th ed, p301, p499) (See all compounds classified as Indicators and Reagents.)|Agents that are capable of inserting themselves between the successive bases in DNA, thus kinking, uncoiling or otherwise deforming it and therefore preventing its proper functioning. They are used in the study of DNA. (See all compounds classified as Intercalating Agents.)|Chemicals and substances that impart color including soluble dyes and insoluble pigments. They are used in INKS; PAINTS; and as INDICATORS AND REAGENTS. (See all compounds classified as Coloring Agents.)

MeSH Entry Terms：

Diiodide, Propidium

Propidium iodide Use and Manufacturing

Propidium iodide is the most common red-fluorescent nuclear stain. It can also be excited using excitation filters appropriate for green-fluorescent dyes.

Propidium Iodide is a phenanthridine compound, a class of compounds with reported activity against several species of trypanosomes. Propidium iodide may be used in flow cytometry to evaluate cell viab ility when used with other dyes that stain viable cells or cells that are early in the apoptosis process.

Used as fluorescent stain for nucleic acids,and used in flow cytometry to evaluate cell viability

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CAS No.: 25535-16-4

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Propidium iodide

CAS No.: 25535-16-4

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