bisBenziMide H 33342 trihydrochloride
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bisBenziMide H 33342 trihydrochloride
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CAS No:
875756-97-1
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Formula:
C27H29ClN6O
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Chemical Name:
bisBenziMide H 33342 trihydrochloride
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Synonyms:
Hoechst 33342 (trihydrochloride);Hoechst 33342 (hydrochloride);CS-1351;Hoechst-33342, HOE 33342;BISBENZIMIDE H 33342 TRIHYDROCHLORIDE;HOE 33342 TRIHYDROCHLORIDE;HOECHST-33342 TRIHYDROCHLORIDE;HOECHST33342 TRIHYDROCHLORIDE;HOE 33342 trihydrochloride;Hoechst 33342 trihydrochloride, >99%;98% (HPLC and TLC)
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CAS No:
Description
Hoechst 33342 is a cell-permeable DNA dye that binds to the minor groove of adenine and thymine-rich sequences. It emits blue fluorescence (excitation 350 nm/emission maximum 461 nm) when bound to double stranded DNA.
bisBenziMide H 33342 trihydrochloride Basic Attributes
489.02
560.162493
P976261J69
DTXSID40236482
Yellow to green powder or solid
Characteristics
350 nm (H2O/DNA); 357 nm (MeOH/H+)
H2O: 20mg/mLphosphate buffer: precipitates
1.7 (20°C)
11.08 ± 0.69, most acidic, temperature: 25 °C; 7.66 ± 0.42, most basic(at 25℃)
Inert atmosphere,Store in freezer, under -20°C
Drug Information
Chemicals that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags. (See all compounds classified as Fluorescent Dyes.)|Drugs used to protect against ionizing radiation. They are usually of interest for use in radiation therapy but have been considered for other purposes, e.g. military. (See all compounds classified as Radiation-Protective Agents.)|Drugs used to potentiate the effectiveness of radiation therapy in destroying unwanted cells. (See all compounds classified as Radiation-Sensitizing Agents.)
2,5'-bi-1h-benzimidazole, 2'-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-, trihydrochloride
bisBenziMide H 33342 trihydrochloride Use and Manufacturing
Bisbenzimide Hoechst 33342 is a specific stain for AT-rich regions of double-stranded DNA and has been shown to displace several known DNA intercalators. This fluorescent dye has been used in sorting living cells based on DNA content, used in flow cytometry for the determination of DNA content, and for the visualization of chromatin distribution in living cells. It has been used to detect BrdU incorporation into cells and in studying the initial stages apoptosis and cellcycle distribution., Chromosomes th at are dividing or replicating will not stain with this dye. ', 'Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry.Excitation max. = 346 nmEmission max. = 460 nm
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