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Streptavidin

Streptavidin structure

Streptavidin 

structure
  • CAS No:

    9013-20-1

  • Chemical Name:

    Streptavidin

  • Synonyms:

    Streptavidin;Polystreptavidin;ArrayIt SuperStreptavidin;9: PN: KR2178813 SEQID: 9 claimed sequence;1350540-26-9;2250217-85-5;2563860-23-9

  • Categories:

    Analytical Chemistry  >  Analysis Reagents

Description

A 60-kDa extracellular protein of Streptomyces avidinii with four high-affinity biotin binding sites. Unlike AVIDIN, streptavidin has a near neutral isoelectric point and is free of carbohydrate side chains.

Streptavidin Basic Attributes

378.71228

376.985

618-481-6

35040090

Characteristics

65.6

White lyophilized powder

Gives a clear solution at 5mg/ml in 0.1M NaCl.is soluble in water at 1 mg/mL, or in phosphate buffered saline (PBS).

2-8°C

Safety Information

NONH for all modes of transport

3

22-36/37/38-21/22

24/25-36-26-36/37

Xn,Xi

P261, P264, P271, P280, P302+P352, P304+P340, P305+P351+P338, P312, P321, P332+P313, P337+P313, P362, P403+P233, P405, P501

H315

|Warning|H315 (100%): Causes skin irritation [Warning Skin corrosion/irritation]|P261, P264, P271, P280, P302+P352, P304+P340, P305+P351+P338, P312, P321, P332+P313, P337+P313, P362, P403+P233, P405, and P501|Aggregated GHS information provided by 3 companies from 2 notifications to the ECHA C&L Inventory. Each notification may be associated with multiple companies.

Drug Information

Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant and Hackh's Chemical Dictionary, 5th ed, p301, p499) (See all compounds classified as Indicators and Reagents.)

Strepavidin

Streptavidin Use and Manufacturing

Applications for enzyme-labeled (HRP, AP) streptavidin:Enzyme immunoassays (EIA), including enzyme-linked immunsorbent assays (ELISA) with biotinylated antibodiesCell and tissue staining (for light microscopy)Western blotting (WB) and other membrane-based biotin detectionApplications for fluorescent (FITC, TRITC or Texas Red* Fluor-labeled) streptavidin:Immunofluorescent assays (IFA)Tissue staining (for fluorescence microscopy)Blot immunostainingCell staining (incl. fluorescent activated cell sorting)

Computed Properties

Molecular Weight:378.7
Hydrogen Bond Donor Count:1
Hydrogen Bond Acceptor Count:4
Rotatable Bond Count:4
Exact Mass:376.98519
Monoisotopic Mass:376.98519
Topological Polar Surface Area:65.6
Heavy Atom Count:20
Complexity:351
Covalently-Bonded Unit Count:2
Compound Is Canonicalized:Yes

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