o-Nitrophenyl β-D-galactopyranoside
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o-Nitrophenyl β-D-galactopyranoside
structure -
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CAS No:
369-07-3
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Formula:
C12H15NO8
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Chemical Name:
o-Nitrophenyl β-D-galactopyranoside
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Synonyms:
β-D-Galactopyranoside,2-nitrophenyl;Galactopyranoside,o-nitrophenyl,β-D-;2-Nitrophenyl β-D-galactopyranoside;o-Nitrophenyl β-D-galactopyranoside;o-Nitrophenyl β-galactoside;o-Nitrophenyl β-D-galactoside;o-Nitrophenyl-β-galactopyranoside;o-Nitrophenol β-D-galactopyranoside;ONPG;NSC 83631;o-NPG;2-Nitrophenyl β-D-galactoside
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CAS No:
Description
ONPG is a colorimetric and spectrophotometric substrate for detection of β-galactosidase activity.
o-Nitrophenyl β-D-galactopyranoside Basic Attributes
301.25
301.25
92207
206-716-1
83631|1947
29400090
Characteristics
145
-1.2
White to very slightly yellow Powder
1.6±0.1 g/cm3
193-194 °C
572.4°C at 760 mmHg
300.0±30.1 °C
1.648
soluble in water.
2-8°C
6.1E-14mmHg at 25°C
-70 º (c=1, H2O)
Safety Information
NONH for all modes of transport
3
36/37/38
22-24/25-36-26
Xi
Stable under normal temperatures and pressures.
P261, P264, P271, P280, P302+P352, P304+P340, P305+P351+P338, P312, P321, P332+P313, P337+P313, P362, P403+P233, P405, P501
H315
o-Nitrophenyl β-D-galactopyranoside Use and Manufacturing
It is used to detect the activity of β-galactosidase and is a chromogenic substrate for β-galactosidase. After being catalyzed by β-galactosidase, a yellow product can be produced, and the absorbance can be detected at a wavelength of 410-420 nm.
Computed Properties
Molecular Weight:301.25
XLogP3:-1.2
Hydrogen Bond Donor Count:4
Hydrogen Bond Acceptor Count:8
Rotatable Bond Count:3
Exact Mass:301.07976644
Monoisotopic Mass:301.07976644
Topological Polar Surface Area:145
Heavy Atom Count:21
Complexity:362
Undefined Atom Stereocenter Count:5
Covalently-Bonded Unit Count:1
Compound Is Canonicalized:Yes
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o-Nitrophenyl β-D-galactopyranoside
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