The story between coagulant and fibrin precipitation

Serum is one of the main specimens tested in clinical biochemical and immunological tests. At present, the methods of obtaining serum samples in medical institutions are mainly obtained by centrifugation after collecting venous blood after the blood is completely coagulated. Under normal circumstances, it takes more than 60 minutes for an isolated blood sample to completely coagulate, or even not coagulate, which is difficult to meet the needs of rapid laboratory testing. Therefore, shortening the coagulation process and quickly separating serum samples are important factors to improve the efficiency of laboratory medicine. The accelerator was born from this. 

Procoagulant principle of blood coagulant:

Activate part of the coagulation factors in plasma and platelets, promote the formation of thromboplastin and fibrin clots, accelerate the contraction of blood clots, and achieve the purpose of rapid serum separation.

In vacuum blood collection tubes containing coagulant, fibrin precipitated filaments and lumps sometimes appear, which is caused by the unstandardized use of coagulation blood collection tubes. Choosing a coagulant with too fast coagulation speed in the preparation of vacuum blood collection tubes will cause fibrin to shrink too fast and fragile red blood cells will easily fragment and cause mild hemolysis. Generally, the precipitation of fibrin has the following reasons:

Use procoagulant blood collection tube or separation gel to promote coagulation blood collection tube. To make the coagulant evenly distributed in the blood, it must be slightly inverted and mixed 4-5 times to make the center of the blood collection tube and the surrounding blood coagulate at the same time. If the blood is not completely coagulated and then centrifuged, it can cause the precipitation of fibrin. The upper part of the serum appeared jelly-like and fragmented, mixed with the blood. Fibrin filaments are caused by the fact that fibrin has not yet fully contracted.

Secondly, when the blood collection tube company prepared the coagulant blood collection tube, the coagulant was not sprayed enough or the prepared water-soluble coagulant was not used up on the same day, and continued use the next day, which resulted in the decrease of the coagulant effectiveness. The fibrinogen in the blood is gradually transformed into insoluble fibrin under the action of the coagulant. If the coagulant dose is insufficient or fails to extend the clotting time, centrifugation can lead to the precipitation of fibrin.

How to prevent fibrin from vacuum blood collection tube

1. The coagulant should be sprayed quantitatively when preparing the coagulant vacuum blood collection tube and dried under the condition of less than 45℃; if it is higher than 50℃, the efficiency of the coagulant may decrease.

2. The coagulant prepared with distilled water or prepared with absolute ethanol must have standardized quality management, and the coagulant shall be sprayed quantitatively.

3. The blood of dialysis patients must be separated from the serum with a tube containing neutralizing heparin to promote the separation of high-quality serum samples.

Desheng’s main products are blood collection tube additives. It has 15 years of R&D and production experience. Coagulant, high-efficiency coagulant powder, heparin sodium, heparin lithium, and serum separation gel are our main products for so many years. With high quality, stable performance and guaranteed after-sales service, everyone is welcome to come to exchange and cooperate.