The place where the virus is held is in the virus preservation solution

Hearing about the virus makes us feel terrible. In particular, last year's new coronavirus has made the whole world smell pale. What does this have to do with our virus preservation solution? Virus preservation solution, as its name implies, is the liquid used to preserve viruses. It is to prevent it from harming us in good condition, so that we can better understand, understand, study and analyze it, and then to overcome it.

A novel coronavirus, hand foot mouth virus and influenza virus are used for sampling, preservation and transportation of virus samples. They can be used to collect tissue samples from throat swabs, nasal swabs or specific parts. Nucleic acid extraction or purification and other clinical experiments.

Generally divided into two types, one is non inactivated, the other is inactivated; the non inactivated type can protect the protein and nucleic acid of the virus, and can preserve the activity of the virus for cultivation and detection; while the inactivated type is to make the virus no longer have physiological activity, lose infection and pathogenicity, but also have reproductive capacity, can inactivate the virus, put an end to secondary infection, and protect the transportation and detection personnel Security.

So how do we choose virus preservation solution? As the virus belongs to parasite, it can not survive in vitro after sampling. If it can not be detected in time, it needs to be stored in virus preservation solution. In order to protect the safety of virus detection environment and detection personnel, we need to use our virus inactivated preservation solution.

But the inactivated preservation solution does not make the virus inactivated, can it be detected? In fact, the purpose of using inactivated preservation solution is to cleave nucleic acid, release nucleic acid, and then detect nucleic acid by real-time fluorescent RT-PCR, so as to judge whether the sample contains virus characteristic nucleic acid, that is, whether it is infected with virus. But the virus structure is single, only contains a nucleic acid and protein, so the detection of characteristic nucleic acid will detect the virus.

Under what circumstances do we use non inactivated virus preservation solution? Non inactivated preservation solution is mainly a modified virus maintenance solution based on transport medium. In this way, the virus has the integrity of protein epitope and nucleic acid in vitro. Of course, if the operation is wrong, there is a certain risk of infection. It is necessary to keep the temperature strictly low for a long time after sampling.

No matter what kind of virus preservation solution is, after virus sampling, the samples can not be transported and preserved at the sample collection site. Therefore, we need to transport and retain the collected virus swabs, otherwise the virus nucleic acid will degrade quickly and the nucleic acid can not be detected. Therefore, it is necessary to add the preservation solution to the sampling tube to prevent the virus nucleic acid from being degraded so quickly 。 At the same time, after sampling should be detected as soon as possible or strictly stored at low temperature to ensure that the detection is accurate. Because of the rapid propagation and mutation of virus, virus preservation solution plays an important role in maintaining the stability of virus samples.