Application of biological buffer caps

Caps, also known as 3-cyclohexylaminopropanesulfonic acid, is a kind of biological buffer. Its appearance is white crystal or powder. Caps can be dissolved in water, pH buffer range is 8.9-10.3, pKa value is 9.6, molecular weight is 243.28, CAS number is 1135-40-6. Caps has a wide range of applications, including DNA / RNA extraction kit, PCR diagnostic kit, biochemical diagnostic kit, welding materials, air conditioning equipment, lithium metal, new coatings, new industrial materials, etc.

In the extraction of nucleic acid, the solution for nucleic acid hybridization can be prepared together with 3 - [(3-choleamidopropyl) dimethylammonium] - 1-propanesulfonate (chaps), 3 - (cyclohexylamino) - 2-hydroxy-1-propanesulfonate (capso), and 2 - (cyclohexylamino) ethanesulfonate (ches). It can reduce the yield of non-specific hybrids and improve the specificity of nucleic acid hybridization, while maintaining the yield of target hybridization products. It has important value in the identification of specific pathogens, diagnosis of genetic diseases, gene sequence analysis and so on.

Caps can be used in all kinds of environment-friendly high-quality waterborne two-component polyurethane coatings. Caps can coexist with active genes (hydroxyl, carboxyl, amine, epoxy) for a long time at room temperature. Caps is widely used in coatings market. In addition, caps can react with aliphatic polyisocyanate under mild conditions and in the presence of tertiary amine neutralizer. The sulfonated urea derivative is a very good emulsifier.

Caps in capillary electrophoresis analysis, caps can be used to prepare running buffer solution (background electrolyte solution) in capillary electrophoresis. As a terminal electrolyte, caps can realize on-line enrichment and separation of 2-nitrobenzoic acid and 3-nitrobenzoic acid. Caps buffer is suitable for electrophoresis buffer of high molecular weight protein (more than 20kD). In the case of low molecular weight protein imprinting, Tris + glycine is generally used, and tris Tricine + EDTA is used when glycine is excluded by protein sequencing.

Caps can also be used in biochemical diagnostic kits, DNA / RNA extraction kits and PCR diagnostic kits, and as a buffer for enzyme chemistry and HPLC separation of basic drugs. Caps is widely used in the membrane transfer process of protein sequencing. Caps can also support alkaline phosphatase activity and inhibit the growth of Aeromonas when pH is 10.5. Caps is soluble in deionized water and used to purify fibronectin after pH is adjusted to 11.0. In addition, caps is also used as eluent in cation exchange chromatography, manufacturing welding materials, air conditioning equipment and raw materials for manufacturing. It can also be used to manufacture fireworks, analytical reagents, heat exchange carriers, pharmaceutical industry, air conditioning, pyrotechnics, dry batteries and other applications.

Desheng bio has been developing and producing biological buffer for many years, and has rich experience. Caps is the core product, which is highly praised in the field of biochemical diagnostic reagents and the market of new paint products. Desheng has been established for decades. The company's products are sold to more than 100 countries around the world. The product quality is good and the price is more favorable. If you are interested, you can call us. Desheng biology welcomes your call.