The application of acridine ester compounds in the field of life sciences is becoming more and more extensive, mainly due to its high luminous efficiency; the reaction conditions are mild, only H2O2 and dilute alkali are needed, and chemiluminescence (CL) can be excited without adding a catalyst. The outstanding feature of the CL reaction mechanism of this type of compound is that the luminescent group involved in the reaction is dissociated from the modified group, the luminous efficiency is not limited by the length of the linking arm, and the photodisintegration effect is small. Through the modification of its structure, the possibility of acridine ester compounds participating in the establishment of ultra-micro immunochemical analysis has been greatly increased.
About the application of acridine ester
1. Study on the determination of tissue-type plasminogen activator activity by acridine ester luminescence method
Thrombosis is one of the main diseases that endanger people's life safety. Among several fibrinolytic drugs that have been studied more, tissue-type plasminogen activator (t-PA) is widely used in medicine due to its specificity of fibrinolysis. Favored by the world. Many tissues of the body contain t-PA, but due to the small amount, it cannot be extracted in large amounts. There are already genetic engineering products used in clinical practice abroad, and the domestic development of t-PA genetic engineering products is stepping up, and there is an urgent need to establish a sensitive, simple, and low-cost detection method. At present, acridine esters have been successfully synthesized in China, and the properties of acridinium esters have been comprehensively analyzed, which provides favorable conditions for the establishment of a sensitive and low-cost t-PA activity quantitative method. This method is used to determine rt-PA expressed by human melanoma cells t-PA (mt-PA) and Chinese hamster ovary cells (CHO cells), and obtain results consistent with the fibrin agar plate assay (FAPA); determine health The changes of t-PA activity in plasma before and after human venous blood flow blockade showed obvious differences. Using this method to determine the plasma t-PA activity of patients with lung cancer, the result was significantly higher than that of normal people. Therefore, this method is expected to be used for clinical diagnosis and basic theoretical research of certain diseases. To
2. A new chemiluminescence analysis system for xanthine oxidase-acetaldehyde-acridine [2]
Under the catalysis of Huangjiayin oxidase, acetaldehyde is oxidized to acetic acid by oxygen in the air, and hydrogen peroxide is produced at the same time; the hydrogen peroxide produced by the enzyme reaction oxidizes the new acridinium ester luminescent agent in an alkaline medium. Methyl-9-(p-methylphthalophenyl) acridinium fluorosulfonate, which produces chemiluminescence.
3. Application of acridinium-labeled antibody to the determination of antibody levels in tuberculosis patients
Acridine antibody conjugates can be used for the determination of antibodies produced by bacteria and viruses against the human body and antibodies produced by the body's own immunity. At present, there are at least 30 kinds of autoimmune diseases recognized. As long as the antigen of the disease is prepared to coat the solid phase, the acridinium ester human IgG antibody marker can be used for determination, which can provide meaningful data for clinical diagnosis and research on pathogenesis.
Desheng has 6 kinds of acridinium ester products with different groups: acridinium ester DMAE-NHS, acridinium ester NSP-DMAE-NHS, acridinium salt NSP-SA, acridinium salt NSP-SA-NHS, acridinyl Hydrazine NSP-SA-ADH, acridinium ester ME-DMAE-NHS. In addition to the chemiluminescence reagents of the acridine ester series, we also have products such as luminol and isoluminol.
