Native Calf Terminal Transferase
9027-67-2
2026-07-09
Coenzymes,Inhibito,Enzymes,Zymogens,Substrates,Native Microorganism Creatine Amidinohydrolase
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Product Description
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Seller Information
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Description
ProductName Native Calf Terminal Transferase Cat No NATE-0692 Description Bovine terminal transferase (TdT) is a primer-dependent polymerase that catalyzes the addition of deoxynucleotides to the 3'-OH terminus of DNA molecules with the release of inorganic phosphate. TdT reacts preferentially with either single-stranded DNA molecules or double-stranded-DNA with 3' overhangs, but procedures have been developed to label blunt ends or 3'-recessive ends. In a reaction mixture, the divalent ion (Co2+, Mn2+, Mg2+) will influence purine and pyrimidine polymerization rate. Activities of TdT are also affected by the bases (dATP, dCTP, dGTP and dTTP) present. Abbr TdT, Native (Calf) Alias TdT Source Calf thymus Species Calf Applications Suitable for: • Addition of homopolymers to vectors, inserts and cDNA for cloning • Labeling the 3′-end of double-and single-stranded DNA with non-radioactive or radioactive labels • Carrying out in vitro mutagenesis by adding single nucleotides to DNA • Use in TUNEL assays Product Overview Bovine terminal transferase (TdT) is a primer-dependent polymerase that catalyzes the addition of deoxynucleotides to the 3′-OH terminus of DNA molecules with the release of inorganic phosphate. TdT reacts preferentially with either single-stranded DNA molecules or double-stranded-DNA with 3′ overhangs, but pr ocedures have been developed to label blunt ends or 3′-recessive ends. In a reaction mixture, the divalent ion (Co2+, Mn2+, Mg2+) will influence purine and pyrimidine polymerization rate. Activities of TdT are also affected by the bases (dATP, dCTP, dGTP and dTTP) present. Form buffered aqueous glycerol solution Enzyme Commission Number EC 2.7.7.31 CAS No 9027-67-2 Molecular Weight mol wt 60 kDa Concentration >5000 U/mL Unit Definition One unit will incorporate 1 nanomole of dATP into acid-precipitable material in one hour at 37°C using d (pT)6 as primer. Storage −20°C Warnings Activity assay uses 200 mM potassium cacodylate, pH 7.2, 4 mM MgCl2, 1 mM 2-mercaptoethanol, 1 mM 3H-dATP, 70 μM d (pT)6, 37°C. Function DNA binding; DNA nucleotidylexotransferase activity; DNA-directed DNA polymerase activity Synonyms DNA nucleotidylexotransferase; terminal deoxyribonucleotidyltransferase; terminal addition enzyme; addase; deoxynucleotidyl terminal transferase; deoxyribonucleic acid nucleotidyltransferase; deoxyribonucleic nucleotidyltransferase; terminal deoxynucleotide transferase; TdT; EC 2.7.7.31; 9027-67-2 category Specialty Chemicals(c26) cas_num 9027-67-2 Basic Info-
Product Name:
Terminal deoxyribonucleotidyltransferase
Other Name:Terminal deoxyribonucleotidyltransferase;Nucleotidyltransferase,terminal deoxyribo-;E.C. 2.7.7.31;Addase;DNA nucleotidylexotransferase;Deoxyribonucleic nucleotidylexotransferase;Deoxyribonucleic acid nucleotidylexotransferase;Terminal deoxynucleotidyltransferase;Deoxynucleotidyl terminal transferase;Terminal deoxynucleotide transferase;TdT;Terminal transferase;DNA terminal transferase;Terminal deoxynucleoside transferase
CAS No.:9027-67-2
InChIKeys:KSJBMDCFYZKAFH-UHFFFAOYSA-N
Molecular Weight:154.304
Exact Mass:153.994
EC Number:232-819-6
Categories:
Characteristics-
PSA:
27.3
XLogP3:1.5
Hazard IdentificationClassification of the substance or mixture
no data available
GHS label elements, including precautionary statements
Pictogram(s) no data available Signal word no data available
Hazard statement(s) no data available
Precautionary statement(s) Prevention no data available
Response no data available
Storage no data available
Disposal no data available
Other hazards which do not result in classification
no data available
Handling and StoragePrecautions for safe handling
Handling in a well ventilated place. Wear suitable protective clothing. Avoid contact with skin and eyes. Avoid formation of dust and aerosols. Use non-sparking tools. Prevent fire caused by electrostatic discharge steam.
Conditions for safe storage, including any incompatibilities
Store the container tightly closed in a dry, cool and well-ventilated place. Store apart from foodstuff containers or incompatible materials.
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Seller Information
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Business Type:
Manufactory
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Main Products:
Coenzymes,Inhibito,Enzymes,Zymogens,Substrates,Native Microorganism Creatine Amidinohydrolase
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Location:
Shirley, New York 11967, USA
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Payment Terms:
TT against copy of documents,D/P
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Average lead Time:
15
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Total Annual Revenue:
$1 million-$2.5 million
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Year of Establishment:
2004
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