Coenzymes,Inhibito,Enzymes,Zymogens,Substrates,Native Microorganism Creatine Amidinohydrolase
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Product Description
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Seller Information
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Description
ProductName D(-)-Luciferin Cat No CSUB-0354 Applications Substrate for firefly luciferase with a Km of approx 2 μM.Used for the luminometric determination of Luc activity in cell extracts. Natural substrate of luciferase from firefly. Used together with firefly luciferase for the determination of ATP using bioluminescence. CAS No 2591-17-5 Molecular Weight 280.32 Storage -20°C Preparation Instructions Working concentration: For the assay of medium concentrations of ATP (10-9 to 10-6 M in the assay cuvette), use 35 to 70 μM D(-)-Luciferin. For the assay of low concentrations of ATP (10-13 to 10-8 M in the assay cuvette), use 350 μM D(-)-Luciferin. For the assay of metabolites convertible to ATP or enzymes which produce ATP, the literature suggests concentrations of D(-)-Luciferin from 35-359 μM.Working solution: Preparation of D(-)-Luciferin solutionTo minimize handling of the unstable compound, prepare a D(-)-Luciferin solution at the approximate concentration desired, then adjust it to the exact concentration on the basis of absorbance at 327 nm. (The absorptivity of D(-)-Luciferin at 327 nm is 18.2 mmol-1 x l x cm-1).For instance, to prepare a 700 M solution of D()-Luciferin:• Add 1.5 mg of D(-)-Luciferin to 5 ml of 70 mM Tris-acetate, pH 7.75 [theoretical concentration = 1.07 mM]• Dilute a portion of that stock solution 20-fold with buffer.• Read the absorbance at 327 nm.• Add buffer to the stock so that a 20-fold dilution gives A327 of 0.637 (concentration of 20-fold dilution = 35 μM; concentration of stock = 700 μM) Synonyms (S)-2-(6-Hydroxy-2-benzothiazolyl)-2-thiazoline-4-carboxylic acid; 4, 5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid; D-Luciferin; Firefly Luciferin Molecular Formula C11H8N2O3S2 MDL Number MFCD00042929 Shipping Conditions dry ice Beilstein Registry Number 30484.0 Substrates Luciferase category Specialty Chemicals(c26) cas_num 2591-17-5 ECHEMI Editorial ReferenceD-luciferin is the natural substrate of luciferases that catalyze the production of light in bioluminescent insects.
Photinus luciferin is a 1,3-thiazolemonocarboxylic acid consisting of 3,5-dihydrothiophene-4-carboxylic acid having a 6-hydroxybenzothiazol-2-yl group at the 2-position. It has a role as a luciferin. It is a member of benzothiazoles, a 1,3-thiazolemonocarboxylic acid and an imidothioate. It is a conjugate acid of a Photinus luciferin(1-). It is an enantiomer of an ent-Photinus luciferin.Basic Info-
Product Name:
Firefly luciferin
Other Name:4-Thiazolecarboxylic acid,4,5-dihydro-2-(6-hydroxy-2-benzothiazolyl)-,(4S)-;2-Thiazoline-4-carboxylic acid,2-(6-hydroxy-2-benzothiazolyl)-,(-)-;4-Thiazolecarboxylic acid,4,5-dihydro-2-(6-hydroxy-2-benzothiazolyl)-,(S)-;(4S)-4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid;Luciferin (firefly);Luciferin;Firefly luciferin;D-(-)-Luciferin;D-Luciferin;(S)-Luciferin (firefly);Beetle luciferin;BrightGlo;(4S)-2-(6-Hydroxybenzothiazol-2-yl)-4,5-dihydrothiazole-4-carboxylic acid;BriteLite substrate;(4S)-2-(6-Hydroxy-1,3-benzothiazol-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid;32810-27-8
CAS No.:2591-17-5
Molecular Formula:C11H8N2O3S2
InChIKeys:InChIKey=BJGNCJDXODQBOB-SSDOTTSWSA-N
Molecular Weight:280.32300
Exact Mass:280.32
EC Number:219-981-3
UNII:5TBB02N29K
HScode:2934999090
Categories:
Characteristics-
PSA:
136.32000
XLogP3:1.38410
Appearance:off-white to light yellow powder
Density:1.81g/cm3
Melting Point:200-204ºC
Boiling Point:473.7ºC at 760mmHg
Flash Point:240.3ºC
Refractive Index:1.865
Storage Conditions:-20ºC
Vapor Pressure:0mmHg at 25°C
Hazard IdentificationClassification of the substance or mixture
Not classified.
GHS label elements, including precautionary statements
Pictogram(s) No symbol. Signal word No signal word
Hazard statement(s) none
Precautionary statement(s) Prevention none
Response none
Storage none
Disposal none
Other hazards which do not result in classification
no data available
Handling and StoragePrecautions for safe handling
Handling in a well ventilated place. Wear suitable protective clothing. Avoid contact with skin and eyes. Avoid formation of dust and aerosols. Use non-sparking tools. Prevent fire caused by electrostatic discharge steam.
Conditions for safe storage, including any incompatibilities
Store the container tightly closed in a dry, cool and well-ventilated place. Store apart from foodstuff containers or incompatible materials.
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Seller Information
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Business Type:
Manufactory
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Main Products:
Coenzymes,Inhibito,Enzymes,Zymogens,Substrates,Native Microorganism Creatine Amidinohydrolase
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Location:
Shirley, New York 11967, USA
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Payment Terms:
TT against copy of documents,D/P
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Average lead Time:
15
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Total Annual Revenue:
$1 million-$2.5 million
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Year of Establishment:
2004
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Country Product Purchase Quantity Date Posted Post an enquiry for this product
