Diaphorase 22 from Recombinant E.coli
9001-18-7
2026-07-07
Coenzymes,Inhibito,Enzymes,Zymogens,Substrates,Native Microorganism Creatine Amidinohydrolase
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Product Description
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Seller Information
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Description
ProductName Diaphorase 22 from Recombinant E.coli Cat No NATE-1938 Description A flavoprotein (FAD). A component of the multienzyme 2-oxo-acid dehydrogenase complexes. In the pyruvate dehydrogenase complex, it binds to the core of EC 2.3.1.12, dihydrolipoyllysine-residue acetyltransferase, and catalyses oxidation of its dihydrolipoyl groups. It plays a similar role in the oxoglutarate and 3-methyl-2-oxobutanoate dehydrogenase complexes. Another substrate is the dihydrolipoyl group in the H-protein of the glycine-cleavage system (click here for diagram), in which it acts, together with EC 1.4.4.2, glycine dehydrogenase (decarboxylating), and EC 2.1.2.10, aminomethyltransferase, to break down glycine. It can also use free dihydrolipoate, dihydrolipoamide or dihydrolipoyllysine as substrate. This enzyme was first shown to catalyse the oxidation of NADH by methylene blue; this activity was called diaphorase. The glycine cleavage system is composed of four components that only loosely associate: the P protein (EC 1.4.4.2), the T protein (EC 2.1.2.10), the L protein (EC 1.8.1.4) and the lipoyl-bearing H protein. Source E. coli Form Lyophilized Enzyme Commission Number EC 1.8.1.4 Activity >150 U/mg protein CAS No 9001-18-7 Contaminants (as Diaphorase activity = 100 %)Adenylate kinase < 0.01 %NADH oxidase < 0.20 % Molecular Weight ca. 110,000 pH Stability 6.0 – 9.0 Michaelis Constant (50 mM HEPES buffer, pH 7.0, at 30 °C)3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) 0.345 mMNADH 0.033 mM Unit Definition One unit of activity is defined as the amount of Diaphorase that forms 1 μmol of NAD+ per minute at 30 °C. Optimum pH 8 Thermal stability No detectable decrease in activity up to 70 °C. Storage Store at -20°C Synonyms LDP-Glc; LDP-Val; dehydrolipoate dehydrogenase; diaphorase; dihydrolipoamide dehydrogenase; dihydrolipoamide:NAD+ oxidoreductase; dihydrolipoic dehydrogenase; dihydrothioctic dehydrogenase; lipoamide dehydrogenase (NADH); lipoamide oxidoreductase (NADH); lipoamide reductase; lipoamide reductase (NADH); lipoate dehydrogenase; lipoic acid dehydrogenase; lipoyl dehydrogenase; protein-6-N-(dihydrolipoyl)lysine:NAD+ oxidoreductase category Enzymes and Coenzymes Drugs(c1513) cas_num 9001-18-7 Basic Info-
Product Name:
Lipoamide dehydrogenase
Other Name:Lipoamide dehydrogenase;Dehydrogenase,lipoamide;E.C. 1.6.4.3;Diaphorase;Lipoyl dehydrogenase;Lipoamide reductase;Dihydrolipoamide dehydrogenase;Dihydrolipoate dehydrogenase;Dihydrolipoic dehydrogenase;Dihydrolipoyl dehydrogenase;Dihydrothioctic dehydrogenase;Lipoate dehydrogenase;Lipoamide dehydrogenase (NADH);Lipoic acid dehydrogenase;Lipoamide oxidoreductase;α-Lipoamide dehydrogenase;E.C. 1.8.1.4;9037-66-5;9037-47-2
CAS No.:9001-18-7
Molecular Weight:0.00000
EC Number:253-474-8
Categories:
Characteristics-
PSA:
0.00000
XLogP3:0.00000
Appearance:light brown-yellow lyophilized powder
Storage Conditions:2-8°C
Hazard IdentificationClassification of the substance or mixture
no data available
GHS label elements, including precautionary statements
Pictogram(s) no data available Signal word no data available
Hazard statement(s) no data available
Precautionary statement(s) Prevention no data available
Response no data available
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Other hazards which do not result in classification
no data available
Handling and StoragePrecautions for safe handling
Handling in a well ventilated place. Wear suitable protective clothing. Avoid contact with skin and eyes. Avoid formation of dust and aerosols. Use non-sparking tools. Prevent fire caused by electrostatic discharge steam.
Conditions for safe storage, including any incompatibilities
Store the container tightly closed in a dry, cool and well-ventilated place. Store apart from foodstuff containers or incompatible materials.
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Seller Information
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Business Type:
Manufactory
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Main Products:
Coenzymes,Inhibito,Enzymes,Zymogens,Substrates,Native Microorganism Creatine Amidinohydrolase
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Location:
Shirley, New York 11967, USA
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Payment Terms:
TT against copy of documents,D/P
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Average lead Time:
15
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Total Annual Revenue:
$1 million-$2.5 million
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Year of Establishment:
2004
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