β-nicotinamide mononucleotide, reduced form, disodium salt(NMNH) 98.0%Min RC00582

Manufacturer: Hangzhou Royalchem Co.,LTD.

Website:  www.cnroyalchem.com

Here is a detailed product description for β-Nicotinamide Mononucleotide, Reduced Form, Disodium Salt (NMNH), CAS 108347-85-9:

1. Chemical Identity & Structure  *  Chemical Name:  β-Nicotinamide Mononucleotide, Reduced Form, Disodium Salt *  Common Abbreviations:  NMNH; rNMNH; Dihydronicotinamide ribotide disodium salt *  CAS Number:  108347-85-9 *  Synonyms:  1-(5-O-Phosphono-β-D-ribofuranosyl)-1,4-dihydronicotinamide disodium salt; Dihydronicotinamide ribonucleotide disodium salt; β-Dihydronicotinamide ribose monophosphate disodium salt *  Molecular Formula:  C₁₁H₁₄N₂Na₂O₈P • xH₂O (Commonly supplied as hydrate, often monohydrate C₁₁H₁₆N₂Na₂O₉P) *  Molecular Weight:  377.18 g/mol (Anhydrous) *  IUPAC Name:  Disodium [(2R,3S,4R,5R)-5-(1,4-dihydro-3-carbamoylpyridin-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate *  Structure:  NMNH consists of a reduced nicotinamide ring (dihydronicotinamide) glycosidically linked to the anomeric carbon of ribose, which is phosphorylated at the 5'-hydroxyl group. The disodium salt neutralizes the phosphate group's acidity. The reduced ring lacks the aromaticity of NMN/NMN⁺.

2. Key Characteristics & Properties  *  Appearance:  Typically supplied as a lyophilized (freeze-dried) powder. *  Color:   P ale yellow to yellow solid .  Crucially, the reduced form (dihydronicotinamide) is inherently unstable and can oxidize upon exposure to air, moisture, or light, often developing a yellowish, pinkish, or reddish hue over time. A significant red/pink color indicates substantial oxidation to NMN⁺.  *  Solubility:  Soluble in water. Solubility in organic solvents is generally poor. Solutions are typically prepared fresh. *  Purity:  ≥9 8 % (HPLC) is common for research-grade material. Purity is assessed primarily by HPLC (UV detection, often at 260 nm or specific for dihydronicotinamide ~340-360 nm). *  Stability:   Highly unstable.  NMNH is extremely sensitive to: *  Oxidation:  Rapidly oxidizes upon exposure to atmospheric oxygen, converting back to NMN⁺. This is the primary degradation pathway. *  Light:  Accelerates oxidation. *  Heat:  Accelerates degradation. *  pH:  Degrades faster under acidic or strongly alkaline conditions. *  Storage:   Must be stored desiccated at ≤ -20°C, preferably ≤ -80°C, under inert atmosphere (argon or nitrogen) and protected from light.  Short-term handling should minimize air exposure. Lyophilized powders are more stable than solutions.

3. Biological Significance & Function  *  Reduced Form of NMN:  NMNH is the two-electron reduced (dihydro) form of β-Nicotinamide Mononucleotide (NMN⁺). *  NAD(P)H Precursor:  Serves as a direct biosynthetic precursor to the reduced forms of the essential pyridine nucleotides, Nicotinamide Adenine Dinucleotide (NADH) and Nicotinamide Adenine Dinucleotide Phosphate (NADPH). *  Role in Redox Metabolism:  While NMN⁺ is a key precursor for NAD⁺ synthesis, NMNH itself is a direct precursor for NADH and NADPH synthesis. NADH and NADPH are the primary cellular reducing agents, essential for: * ATP production via oxidative phosphorylation (NADH). * Anabolic biosynthesis (e.g., fatty acid, cholesterol, nucleotide synthesis - NADPH). * Antioxidant defense systems (e.g., glutathione reductase - NADPH). * Detoxification reactions (Cytochrome P450 systems - NADPH). *  Enzymatic Conversion:  NMNH is phosphorylated to NADH by NAD⁺ kinase or can be adenylated to NADH. It can also be reduced to NADPH.

4. Primary Applications (Research Use Only)  *  Biochemistry Research:  Fundamental studies on pyridine nucleotide biosynthesis, metabolism, and interconversion pathways. *  Enzymology Research:  Investigation of enzymes that utilize or produce NMNH, NADH, or NADPH as substrates, cofactors, or products (e.g., dehydrogenases, reductases, NAD⁺ kinases). *  Cellular Metabolism Studies:  Exploring the role and dynamics of reduced pyridine nucleotide pools (NADH/NADPH) in cellular energy metabolism, redox balance, and signaling. Investigating the potential impact of direct NMNH supplementation on cellular NADH/NADPH levels. *  Redox Signaling Research:  Studying the influence of NADH/NADPH levels and the NAD⁺/NADH & NADP⁺/NADPH ratios on cellular signaling pathways and gene expression. *  Comparative Studies:  Comparing the biological effects and metabolism of NMNH vs. its oxidized counterpart (NMN⁺) and other NAD⁺ precursors (e.g., NR, NAM). *  (Potential) Nutraceutical/Pharmaceutical Research:   Emerging and highly experimental  research into the potential therapeutic effects of elevating NADH/NADPH levels via NMNH supplementation in models related to energy deficits, oxidative stress, mitochondrial dysfunction, aging, or metabolic disorders.  This is preclinical research; efficacy and safety in humans are not established.

5. Handling & Safety  *  For Research Use Only:  Not for diagnostic, therapeutic, or human consumption. *  Stability Handling:  Due to extreme sensitivity to oxidation, handling must be meticulous. Reconstitute only immediately before use with degassed buffers if possible, under inert atmosphere. Minimize exposure to air, light, and heat. Aliquot solutions and store unused aliquots at ≤ -80°C. *  Safety Data Sheet (SDS):  Consult the specific SDS provided with the product for detailed hazard information, handling precautions, first aid measures, and disposal procedures. *  General Precautions:  Wear appropriate personal protective equipment (PPE) including lab coat, gloves, and safety glasses. Avoid inhalation, ingestion, and skin/eye contact.