Kanamycin A sulfate

Kanamycin A sulfate More Info

1. Product Description

Kanamycin A Sulfate is the sulfate salt of Kanamycin A, the principal and most active component of the kanamycin complex. It is an aminoglycoside antibiotic derived from the bacterium Streptomyces kanamyceticus. This compound is a white to off-white crystalline powder that is highly soluble in water but insoluble in alcohol, acetone, and ether. It is renowned for its potent bactericidal activity and is a critical raw material in the pharmaceutical and biotechnology industries.

2. Mechanism of Action

The mechanism of action of Kanamycin A involves the inhibition of bacterial protein synthesis. It irreversibly binds to the 30S subunit of the bacterial ribosome. This binding interferes with the initiation complex formation between mRNA and the ribosome and causes the misreading of the genetic code. Consequently, the bacteria produce non-functional proteins, which ultimately leads to cell death.

3. Antibacterial Spectrum

Kanamycin A Sulfate exhibits strong activity against a wide range of aerobic gram-negative bacilli. This includes common pathogens such as Escherichia coli, Klebsiella pneumoniae, Proteus species, Enterobacter aerogenes, and Serratia marcescens. It is also highly effective against Mycobacterium tuberculosis, making it an important agent in tuberculosis treatment regimens. While effective against some gram-positive bacteria like Staphylococcus aureus, it is generally ineffective against anaerobes and most streptococci.

4. Key Applications

Pharmaceutical API: It is primarily used in the manufacturing of injectable drugs for the treatment of severe systemic infections caused by susceptible strains.

Biological Research: Due to its selective toxicity, it is widely used as a selection agent in molecular biology and cell culture. It is commonly used with kanamycin-resistant plasmids to maintain selective pressure on bacterial cultures.

5. Quality and Specifications

Our Kanamycin A Sulfate is manufactured under strict GMP conditions and meets the rigorous standards of international pharmacopoeias, including the USP (United States Pharmacopeia), EP (European Pharmacopoeia), BP (British Pharmacopoeia), and CP (Chinese Pharmacopoeia).

The typical quality characteristics are as follows:

Assay: The potency is typically not less than 760 micrograms per milligram, calculated as Kanamycin base.

Purity Profile: The content of Kanamycin A is typically not less than 95.0%, while the content of the related substance Kanamycin B is strictly controlled to be not more than 4.0%.

Physical Properties: The pH value of a 50mg/mL solution in water is typically between 6.0 and 8.0.

Impurities: The water content is controlled to be not more than 6.0%, and heavy metals are limited to not more than 20 parts per million.

6. Storage and Handling

The product is stable under normal temperatures and pressures. It should be stored in a cool, dry place and protected from light to maintain its integrity and potency.