Product Detail  

Botulinum Toxin Type A (BoNT-A) – Full Detailed Product Description

Product Overview

Core Basic Specifications

• Serotype: Botulinum Neurotoxin Type A (BoNT/A)
• Molecular Weight: 150 kDa holotoxin (100 kDa heavy chain + 50 kDa light chain linked by disulfide bond)
• Complex Form: Purified neurotoxin complex with accessory hemagglutinin proteins
• Purity: ≥98% verified via SDS-PAGE, HPLC and mass spectrometry
• Potency: Standardized biological activity units (U), calibrated against international reference toxin
• Appearance: White sterile vacuum-dried lyophilized powder
• Solvent for reconstitution: Sterile 0.9% sodium chloride or bacteriostatic benzyl alcohol water
• Standard Vial Specifications: 50U / 100U / 200U per sealed vial
• Quality Documents: Batch COA, HPLC chromatogram, SDS-PAGE report, potency assay record supplied
• Shelf Life: 36 months under recommended long-term storage
• Storage Conditions: Sealed and stored at -20°C, avoid repeated freeze-thaw cycles, protect from direct light and moisture; reconstituted solution stable at 2–8°C within 4 hours only

Mechanism of Action

1. High-Affinity Binding: The heavy chain of toxin selectively binds to SV2 glycoprotein and ganglioside receptors on presynaptic cholinergic nerve terminals.
2. Endocytosis Internalization: The whole toxin-receptor complex is engulfed into nerve cells via endocytic vesicles.
3. Light Chain Translocation: Acidic vesicle environment triggers conformational change; the catalytic zinc-metalloprotease light chain crosses the vesicle membrane into cell cytoplasm.
4. SNARE Protein Cleavage: Light chain specifically cleaves SNAP-25, a core SNARE protein responsible for neurotransmitter vesicle docking.
5. Acetylcholine Release Blockade: Damaged SNARE complex cannot fuse with cell membrane, stopping acetylcholine secretion, leading to reversible muscle relaxation and temporary paralysis.

Main Research Applications

1. Neuromuscular junction physiology & spastic muscle disorder preclinical models
2. Chronic pain, migraine and sensory nerve sensitization mechanism research
3. Anti-wrinkle, dermatological muscle relaxation pharmaceutical formulation study
4. Autonomic nervous system secretion regulation (excessive sweating, gland overactivity)
5. SNARE protein and synaptic vesicle trafficking cell biology experiments
6. Novel toxin engineering, antibody neutralization and antitoxin drug development
7. Comparative study of different botulinum serotypes (A/B/E)

Product Advantages

1. High Biological Activity & Stable Potency
   
   
   Standardized fermentation and multi-stage chromatographic purification eliminate inactive protein fragments; batch-to-batch potency deviation is controlled within 5%.
2. Low Endotoxin & Sterile Processing
   
   
   Produced under sterile GMP-grade lab environment, 0.2μm sterile filtration before lyophilization, meeting in vitro cell and animal injection experiment standards.
3. Complete Traceable Test Data
   
   
   Every batch carries full test records including purity, protein concentration, mouse LD50 potency assay and impurity inspection, supporting laboratory filing and third-party verification.
4. Convenient Reconstitution & Stable Lyophilized Form
   
   
   Vacuum freeze-drying removes moisture completely to prevent toxin degradation during long-distance transportation; dissolves rapidly without insoluble residue.

Critical Safety Disclaimer

Short Bullet Points for E-commerce Listing

• Botulinum Toxin Type A (BoNT-A), high-potency neurotoxin complex for lab research
• ≥98% purity tested by HPLC, SDS-PAGE and mass spectrum
• Standard potency 50U/100U/200U sterile lyophilized vials
• Blocks SNAP-25 protein to inhibit acetylcholine release at neuromuscular junctions
• Widely used for neuromuscular, pain, synaptic cell biology and pharmaceutical research
• Complete COA, potency assay and identification reports provided per batch
• Long-term storage at -20°C, 3-year shelf life

  About us   

Why choose us