High Purity NAD+ Nicotinamide Adenine Dinucleotide Lyophilized Research Material

  Product Detail  

Brief Overview

Core Research Application & Functional Features

1. Permitted Research Scopes Only

• Cellular energy metabolism and redox reaction mechanism cell culture tests
• Aging-related cell activity and cell survival cycle comparative lab trials
• Mitochondrial function regulation and energy consumption pathway observation experiments
• Enzyme activity detection test for dehydrogenase related biological research
• Oxidative stress balance and cell anti-oxidation capacity evaluation research
• Gene expression regulation and DNA repair related academic biochemical projects
• Contrast experiments of coenzyme concentration gradient influence on cell model indicators

2. Unique Research Advantages

1. Core Endogenous Coenzyme Function
   
   
   NAD+ exists inside all natural living cells as a key hydrogen carrier to support intracellular redox circulation. It serves as the core experimental substrate for all metabolism-related cell tests, providing intuitive and repeatable data for exploring energy transfer rules inside biological carriers.
2. Stable Freeze-Dried Solid Form
   
   
   Professional low-temperature vacuum dehydration processing locks complete molecular activity without structural degradation during storage and transportation. It avoids activity attenuation problems of liquid medium raw materials, greatly extending usable cycle for lab stock management.
3. Wide Matching Compatibility With Cell Culture Systems
   
   
   This material can fully blend with standard cell culture medium and PBS buffer liquid, compatible with most conventional mammalian cell, microorganism and tissue slice test systems, no extra interfering impurities introduced into cell culture environment.
4. Clear Concentration Gradient Experimental Performance
   
   
   Adjustable working concentration range for diversified lab designs; obvious gradient changes of cell metabolic indicators can be observed with different addition doses, convenient for quantitative data statistics and academic paper result analysis.
5. Low Interference Impurity Profile
   
   
   Strict multi-stage purification workflow minimizes irrelevant residual substances inside finished material. It will not generate extra noise signals in spectrophotometry and fluorescence detection equipment, ensuring high clarity test data output.
6. Uniform Batch-to-Batch Consistency
   
   
   Standardized freeze-drying and purification production workflow ensures identical purity, dissolving performance and molecular activity across all production lots. No test result deviation caused by inconsistent raw material quality, fully fit for parallel comparison experiments and repeated result verification.

Complete Standard Specification Parameters

Basic Product Identification Info

• Full Product Name: β-Nicotinamide Adenine Dinucleotide (NAD+)
• Common Alias: Coenzyme I, β-DPN
• CAS Reference Number: 53-84-9
• Molecular Formula: C₂₁H₂₇N₇O₁₄P₂
• Theoretical Molecular Weight: 663.4 Da
• Visual Form: Uniform pale off-white freeze-dried solid, free of clumps, discoloration, tiny foreign particles or visible impurities
• Odor Characteristic: Completely odorless
• Dissolving Performance: Fully blendable with lab bacteriostatic purified water, cell culture medium and PBS buffer liquid; cannot mix with alcohol-based lab liquid medium
• Post-dissolving Liquid State: Clear, light yellow transparent solution without suspended particles, turbidity or precipitate after full dissolution
• pH Value of 10mg/mL Mixed Liquid: 5.0 ~ 7.0
• Residual Moisture Content Test Index: ≤ 2.5% (tested via Karl Fischer titration method per batch)
• Endotoxin Level: < 0.1 EU/mg, fully comply with global universal safety standards for cell and animal lab research
• Heavy Element Residue Control Limit: Lead ≤10ppm, Arsenic ≤2ppm, Cadmium ≤1ppm, Mercury ≤1ppm
• Microbe Control Standard: Total aerobic microbes <10 CFU/mg, yeast and mold <5 CFU/mg; zero harmful pathogenic bacteria detected in all batches
• Molecular Chain Integrity: Spectrum matching test carried out for every batch to confirm complete intact dinucleotide chain without breakage or truncation

Available Sealed Vial Sizes (No bulk kilogram supply)

• NAD-100: 100mg freeze-dried coenzyme material per vacuum-sealed neutral glass vial
• NAD-500: 500mg freeze-dried coenzyme material per vacuum-sealed neutral glass vial
• NAD-1000: 1000mg freeze-dried coenzyme material per vacuum-sealed neutral glass vial

Strict Production & Inspection Quality Control Standards

1. Core High Purity Benchmark
   
   
   Guaranteed HPLC chromatographic purity of finished coenzyme material ≥99.0%. Complete spectrum chromatogram records are attached to each batch test report. Single unknown individual impurity ≤0.3%, total combined unknown impurities ≤1.0%. Testing adopts C18 reverse-phase chromatographic column with 260nm UV wavelength detection for precise purity numerical measurement.
2. Mandatory Full Batch Testing Items

• Spectrum molecular weight matching test, ensuring measured molecular weight error within ±5 Da compared with theoretical standard value
• Full molecular structure verification to confirm complete matching with standard NAD+ dinucleotide chain structure
• Trace residual substance testing following ICH Q3C standardized limits
• Constant temperature & humidity accelerated stability test for shelf life effectiveness validation
• Coenzyme biological activity detection to lock stable and consistent lab research performance
• 100% manual visual full inspection of all finished sealed vials before shipment to screen out defective goods
• Complete batch full traceability management system covering raw material incoming records, production operation logs, full-item inspection data and outgoing delivery archive files

Standard Step-by-Step Dissolving Operation Guidance

1. Slowly pour prepared liquid medium along the inner wall of the glass vial to avoid massive foaming generation
2. Fully tighten the vial cap, rotate and swirl gently in circular motion until all freeze-dried solid material dissolves completely
3. Do not shake the vial violently; fierce agitation will break complete molecular chain structure and weaken stable coenzyme activity
   
   
   Suggested Working Concentration Range For Cell Model Research: 1μM – 500μM, adjustable flexibly based on customized experiment design and cell strain species

Storage Environment & Valid Usage Period Rules

Unopened Original Vacuum-Sealed Vials

• Long-term storage environmental condition: Keep fully sealed vials stored at -20°C ~ -80°C, completely shielded from direct light and high moisture
• Valid storage duration under constant -20°C freezing environment: 12 months
• Valid storage duration under constant -80°C ultra-low temperature environment: 36 months
• Short transit stability tolerance: Maintain stable molecular activity for up to 7 days under ambient temperature below 25°C
• Forbidden storage behavior: Frequent repeated temperature fluctuation, long-time direct sunlight exposure

Liquid Mixed With Matching Culture Medium / Purified Water

• Storage condition after dissolution: Place inside refrigerator under 2°C ~ 8°C refrigeration environment, fully shaded
• Suggested full usage time limit: Consume all mixed liquid within 14 days after preparation completion
• Strict prohibition: Do not freeze diluted mixed liquid; repeated freezing and thawing cycles will permanently damage coenzyme molecular stability and lose biological activity

Shipping & Receiving Handling Reminders

1. Small trial vial orders can be delivered via standard international express for short-distance cross-border transit
2. Large quantity multi-vial orders are strongly suggested to choose temperature-insulated protective packaging to maintain stable molecular state during long-distance overseas transportation
3. Complete supporting shipping documents will be delivered together with all parcels, including unified technical data sheets and batch exclusive test records for customs inspection and internal lab filing use

Lab Operation Safety Reminders

1. Wear disposable nitrile protective gloves, transparent safety goggles and dust-proof lab masks during powder weighing and dissolving operating procedures
2. Avoid direct skin contact, eye contact and inhalation of dry freeze-dried coenzyme solid powder
3. In case of accidental contact with skin or eye tissue, flush the affected area thoroughly with plenty of clean running water immediately
4. Dispose of residual solid powder and waste mixed liquid strictly following internal waste management rules of your laboratory; do not pour residual liquid directly into domestic drainage pipelines or general household waste bins

Important Usage Restriction Statement

Free Supporting Technical Documents Provided For All Orders

1. Batch exclusive test report with full HPLC spectrum purity test data records
2. Full-version unified technical data sheet covering all specifications, dissolving operation steps and standard storage rules
3. Complete molecular structure schematic diagram and structure verification file
4. Full inspection records of heavy element, endotoxin and microbe testing indexes
5. Original raw data of long-term molecular stability accelerated testing
   
   
   Customized lab dedicated experiment operation guidance documents can be provided upon customer request for institutional internal research archive sorting and lab safety training use
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