Cyanidin-3-O-rhamnoside chloride 98% Purity Anthocyanin Reference Standard for Natural Product Analysis and Antioxidant Activity Research CAS 38533-30

• Product Overview

  Cyanidin-3-O-rhamnoside chloride (CAS 38533-30-1) is a high‑purity anthocyanin reference standard featuring cyanidin as the aglycone with a rhamnose moiety at the 3‑position. The molecular formula is C₂₁H₂₁ClO₁₀ with a molecular weight of 468.84 g/mol. Cyanidin is one of the most abundant anthocyanidins in nature, responsible for the red to purple coloration in many fruits, flowers, and vegetables. As a chloride salt, this compound exhibits enhanced stability and solubility in aqueous solutions, making it suitable for chromatographic analysis. This compound is naturally found in various plant species, notably in berries, cherries, plums, and red grapes, and has been extensively studied for its potent antioxidant, anti‑inflammatory, and cardioprotective properties. As a reference standard, it is essential for the identification and quantification of anthocyanins in plant extracts, food products, and nutraceuticals using HPLC‑DAD and UPLC‑MS/MS methods.

  Synthetic Process / Isolation Method

  This compound is typically isolated from natural plant sources through a series of extraction and chromatographic purification steps:

  • Extraction: Plant materials rich in anthocyanins (such as berries, cherries, or red grapes) are extracted with acidified methanol or ethanol to stabilize the anthocyanin structure.

  • Partitioning: The crude extract is subjected to liquid‑liquid partitioning to concentrate the anthocyanin fraction.

  • Column Chromatography: The enriched fraction undergoes repeated column chromatography using Amberlite XAD‑7, Sephadex LH‑20, or preparative HPLC with acidic mobile phases.

  • Recrystallization: The purified compound is recrystallized to achieve ≥98% purity.

  • Quality Confirmation: The final product is rigorously characterized by HPLC‑PDA, ¹H‑NMR, ¹³C‑NMR, and high‑resolution mass spectrometry.

  Mechanism of Action

  As an anthocyanin compound, cyanidin-3-O-rhamnoside chloride exerts its biological activities through multiple mechanisms:

  • Antioxidant Activity: The phenolic hydroxyl groups effectively scavenge free radicals and chelate metal ions, protecting cells from oxidative stress. This action is crucial in preventing cellular damage associated with various diseases, including cancer and cardiovascular conditions.

  • Anti‑inflammatory Activity: Modulates inflammatory signaling pathways including NF‑κB, reducing the production of pro‑inflammatory cytokines such as TNF‑α, IL‑1β, and IL‑6.

  • Cardioprotective Effects: Improves endothelial function, reduces oxidative damage to vascular tissues, and protects against myocardial ischemia‑reperfusion injury.

  • Neuroprotective Effects: Protects neuronal cells from oxidative damage and may have therapeutic potential in neurodegenerative diseases.

  Quality Specifications

  Our Cyanidin-3-O-rhamnoside chloride is manufactured under strict quality control conditions: - Purity: ≥98.0% determined by HPLC - Identity confirmed by NMR and mass spectrometry - Water content: ≤1.0% - Heavy metals: ≤20 ppm - Each batch accompanied by comprehensive Certificate of Analysis (COA) - Stable for 24 months when stored at -20°C in sealed containers protected from light and moisture

  Packaging & Shipping

  We offer flexible packaging options to meet diverse customer needs: - Research quantities: 2mg, 5mg, 10mg amber glass vials with PTFE‑lined caps, purged with inert gas - Bulk quantities: Available upon request - Custom packaging available upon request - Shipped with blue ice for temperature‑sensitive orders - International shipping available with proper documentation

Jinan Topfull Chemical Co., Ltd. is an international company rooted in China, specializing in the export of chemical products.

Our company is committed to making every effort to provide ideal chemical products to potential customers worldwide, including but not limited to: organic/inorganic reagents, biological reagents, plant extracts, pharmaceutical intermediates, active pharmaceutical ingredients (APIs), reference standards, reference substances, peptides, and more. Meanwhile, we have established long-term and stable cooperative relationships with university laboratories and off-campus research institutions, enabling us to offer tailored product services based on customers' actual needs.

We currently support customized services for most product categories, with supply quantities ranging from milligram (mg), gram (g) to kilogram (kg) levels, as well as wholesale. Product purity covers the full spectrum from 90% to 99.9%, providing customers with one-stop convenient services.

To date, our products have been exported to developed countries such as the United States, Australia, Canada, Mexico, as well as EU member states including France, Germany, and many other countries and regions such as Nigeria, Ghana, Southeast Asia, and other parts of Asia. Our product quality and services have consistently received high praise.

Looking ahead, our company will continue to serve as a bridge connecting China and the world, delivering high-quality products and services to global customers.