Pulsatilla saponin E4 98% Purity Triterpenoid Saponin Reference Standard for Natural Product Analysis and Anti-inflammatory/Antitumor Research CAS 141

Product Overview

Pulsatilla saponin E4 (CAS 1415553-83-1) is a high‑purity triterpenoid saponin reference standard featuring oleanolic acid as the aglycone with a complex pentasaccharide chain attached at the 3‑position. The molecular formula is C₅₉H₉₆O₂₈ with a molecular weight of 1253.41 g/mol. The sugar chain consists of five monosaccharide units arranged in a specific sequence: α‑L‑rhamnose-(1→6)-β-D‑glucose-(1→4)-β-D‑glucose-(1→3)-α‑L‑rhamnose-(1→2)-α‑L‑arabinose. This compound is one of the major bioactive saponins isolated from the roots of Pulsatilla chinensis (Chinese anemone or Bai Tou Weng), a well‑known medicinal plant in traditional Chinese medicine used for the treatment of dysentery, amoebiasis, and inflammatory conditions. As a research‑grade reference standard, it is essential for the identification and quantification of triterpenoid saponins in plant extracts and herbal products using HPLC‑DAD and UPLC‑MS/MS methods.

Synthetic Process / Isolation Method

This compound is typically isolated from the roots of Pulsatilla chinensis through a series of extraction and chromatographic purification steps:

• Extraction: Dried roots of Pulsatilla chinensis are extracted with aqueous ethanol or methanol under reflux.

• Partitioning: The crude extract is subjected to liquid‑liquid partitioning with n‑butanol to concentrate the saponin fraction.

• Column Chromatography: The enriched fraction undergoes repeated column chromatography using silica gel, macroporous resin (D101), or Sephadex LH‑20, eluting with gradient solvent systems such as chloroform‑methanol‑water.

• Preparative HPLC: Final purification is achieved using preparative HPLC with a C18 reversed‑phase column.

• Recrystallization: The purified compound is recrystallized to achieve ≥98% purity.

• Quality Confirmation: The final product is rigorously characterized by HPLC‑PDA, ¹H‑NMR, ¹³C‑NMR, 2D‑NMR (COSY, HMBC, HSQC), and high‑resolution mass spectrometry.

Mechanism of Action

As a triterpenoid saponin, pulsatilla saponin E4 exerts its biological activities through multiple mechanisms:

• Anti‑inflammatory Activity: Modulates inflammatory signaling pathways including NF‑κB and MAPK, reducing the production of pro‑inflammatory cytokines such as TNF‑α, IL‑1β, and IL‑6.

• Antitumor Activity: Induces apoptosis in cancer cells through the activation of caspase‑dependent pathways and modulation of mitochondrial membrane potential. Studies have shown that pulsatilla saponins exhibit cytotoxic effects against various human cancer cell lines including HeLa, MCF‑7, and HepG2.

• Immunomodulatory Effects: Enhances immune function by activating macrophages and promoting the production of immunomodulatory cytokines.

• Antimicrobial Activity: Exhibits inhibitory effects against certain bacterial and fungal strains.

Quality Specifications

Our Pulsatilla saponin E4 is manufactured under strict quality control conditions: - Purity: ≥98.0% determined by HPLC - Identity confirmed by NMR and mass spectrometry - Water content: ≤1.0% - Heavy metals: ≤20 ppm - Each batch accompanied by comprehensive Certificate of Analysis (COA) - Stable for 24 months when stored at 2‑8°C in sealed containers protected from light and moisture

Packaging & Shipping

We offer flexible packaging options to meet diverse customer needs: - Research quantities: 2mg, 5mg, 10mg amber glass vials with PTFE‑lined caps, purged with inert gas - Bulk quantities: Available upon request - Custom packaging available upon request - Shipped at ambient temperature for routine orders - Cold chain shipping available upon request for temperature‑sensitive orders - International shipping available with proper documentation

Jinan Topfull Chemical Co., Ltd. is an international company rooted in China, specializing in the export of chemical products.

Our company is committed to making every effort to provide ideal chemical products to potential customers worldwide, including but not limited to: organic/inorganic reagents, biological reagents, plant extracts, pharmaceutical intermediates, active pharmaceutical ingredients (APIs), reference standards, reference substances, peptides, and more. Meanwhile, we have established long-term and stable cooperative relationships with university laboratories and off-campus research institutions, enabling us to offer tailored product services based on customers' actual needs.

We currently support customized services for most product categories, with supply quantities ranging from milligram (mg), gram (g) to kilogram (kg) levels, as well as wholesale. Product purity covers the full spectrum from 90% to 99.9%, providing customers with one-stop convenient services.

To date, our products have been exported to developed countries such as the United States, Australia, Canada, Mexico, as well as EU member states including France, Germany, and many other countries and regions such as Nigeria, Ghana, Southeast Asia, and other parts of Asia. Our product quality and services have consistently received high praise.

Looking ahead, our company will continue to serve as a bridge connecting China and the world, delivering high-quality products and services to global customers.