Kisspeptin-10 (KP-10) CAS 374675-21-5 High Purity Stable Research Lyophilized Peptide Powder

Kisspeptin-10, commonly abbreviated as KP-10, also named Metastin (45-54), with CAS No.374675-21-5, is a synthetic human-derived decapeptide, corresponding to the C-terminal active fragment of KISS1 gene expression product. Its complete amino acid sequence is Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH₂, molecular formula C₆₃H₈₃N₁₇O₁₄, molecular weight 1302.45. Kisspeptin-10 is a high-affinity endogenous ligand for KISS1R receptor, also known as GPR54, a G-protein coupled receptor widely distributed in multiple tissues. It is extensively adopted in neuroendocrine mechanism research, hypothalamic-pituitary-gonadal axis related research, cell receptor binding test, signal transduction exploration and other in vitro biochemical and cell experiments. Our Kisspeptin-10 is manufactured with mature Fmoc solid-phase peptide synthesis (SPPS) technology. The whole synthetic workflow is strictly controlled, including amino acid coupling, cleavage, purification, desalting, lyophilization and packaging. The finished product is delivered as lyophilized powder. Compared with longer kisspeptin fragments such as kisspeptin-54, KP-10 retains the core receptor binding motif while possessing smaller molecular size, which greatly facilitates laboratory operation and reduces unnecessary interference factors in structure-activity relationship research. Many research papers choose Kisspeptin-10 as preferred peptide reagent for related in-vitro tests, making it a popular raw material for academic institutions and biotech research laboratories.

The appearance of Kisspeptin-10 is white to off-white lyophilized solid powder. The purity tested by RP-HPLC can reach ≥99.0%. The mainstream commercial specifications are 5mg and 10mg per crimp amber vial, and customized packaging quantity can be negotiated for bulk laboratory procurement. Our full quality control test items cover multiple dimensions: appearance visual inspection, RP-HPLC purity detection, LC-MS molecular weight identity confirmation, Karl Fischer water content test, residual amino acid assay, analysis of truncated peptide fragments, oxidative impurities and deamidation impurities. Solubility property: it can dissolve in sterile aqueous solvent, PBS buffer solution within suitable pH range, and normal saline solution. The lyophilized solid powder has moderate hygroscopicity, so moisture isolation is required during storage and transportation. Under recommended storage environment, the lyophilized powder can maintain stable physicochemical indicators for a long time. Every specification indicator is fully recorded in batch COA documents. Each batch product will go through full testing before delivery to ensure all indicators meet preset specifications. Customers can obtain complete test reports together with goods, which can be directly used for laboratory filing, project audit and experimental data traceability.

1 Molecular Structure Advantage

Our Kisspeptin-10 retains the minimal core binding sequence of kisspeptin family, and C-terminal amidation modification plays a key role for peptide stability and receptor binding capacity. C-terminal amidation protects peptide chain from degradation by carboxypeptidase, effectively preventing the cleavage of peptide terminal amino acid residues, and maintaining high binding affinity towards KISS1R receptor. Within the peptide sequence, there are tryptophan and asparagine residues, which are sensitive to oxidation and deamidation. Our optimized synthetic and post-treatment route minimizes the risk of residue modification during production. Compared with longer kisspeptin variants, this decapeptide owns smaller molecular volume, easier for buffer preparation, incubation and receptor binding in cell experiments. The optimized synthesis route greatly reduces by-product generation in peptide chain assembly stage, ensuring complete and intact amino acid sequence of the final peptide product. This structural feature makes KP-10 highly suitable for structure-activity relationship research, dose-response curve testing and receptor activation mechanism study.

2 High Purity & Low Impurity Advantage

We adopt multi-step reversed-phase chromatographic purification technology for Kisspeptin-10. This process efficiently removes truncated peptide fragments, oxidative by-products, deamidation impurities, residual protective groups and synthesis reagents. The RP-HPLC purity of finished product reaches ≥99.0%. Low impurity background greatly reduces non-specific binding and unexpected interference in cell and biochemical experiments. Consistent impurity profile among different batches ensures that researchers can obtain stable, repeatable experimental data in continuous long-term research projects. Trace impurities are strictly controlled within acceptable limits, avoiding impurity-induced false positive or false negative results during receptor binding assay and signal pathway test. Each batch impurity profile is recorded in COA, allowing customers to evaluate impurity influence on their experimental system before starting laboratory tests.

3 Stable Supply & Flexible Order Advantage

Specialized independent peptide production line supports continuous and scalable manufacturing of Kisspeptin-10. Stable production capacity effectively avoids stock shortage and long lead-time problems for overseas laboratories. We support multiple order modes, including small trial sample order for preliminary experiment and large bulk procurement for long-term research project. Custom packaging scheme, batch quantity and labeling requirement can be negotiated according to laboratory needs. Our international sales team handles inquiry, quotation, order confirmation and shipment coordination efficiently. Fast order processing and shipment arrangement are available for overseas purchasers. We can prepare proper export documents required for cross-border customs clearance, helping buyers finish import procedure smoothly.

Lyophilization technology is the core method to maintain Kisspeptin-10 long-term stability. The freeze-drying procedure removes most free water inside peptide cake, inhibiting two main degradation pathways of peptide: peptide hydrolysis and oxidative degradation. Since Kisspeptin-10 peptide chain contains tryptophan residue susceptible to photooxidation and asparagine residue prone to deamidation under high humidity, our workshop strictly controls oxygen concentration and humidity environment during lyophilization, filling and sealing procedures. We carry out systematic stability investigation referring to ICH Q1A and Q1B guidelines, including long-term stability test, accelerated stability test and photostability test. Multiple batches of finished products are placed under preset test conditions, sampled at scheduled time points and tested for appearance, HPLC purity, moisture content and impurity change. Test results demonstrate that under -20℃, dry and dark sealed storage, product purity stays within specification, and degradation impurities accumulate slowly. The sealed amber crimp vial can effectively block ultraviolet light, isolate moisture and oxygen from ambient air. Even during cross-border cold-chain long-distance transportation, molecular integrity and purity of Kisspeptin-10 can remain stable, lowering quality fluctuation risks caused by temperature and humidity changes in long-distance logistics.

Every batch of Kisspeptin-10 follows standardized SOP in synthesis, purification, lyophilization and packaging. Each production step is recorded completely for traceability. Before delivery, each batch will complete full QC inspection: appearance check, HPLC purity analysis, LC-MS molecular weight identification, Karl Fischer water detection, related impurity analysis. Our QC team strictly controls batch-to-batch variation, to guarantee consistent quality between different production batches. Batch-specific COA certificate is provided with each shipment, containing complete raw test data, convenient for laboratory record and audit. All finished products will be rechecked before warehouse-out, verifying vial sealing status, label integrity, appearance of lyophilized cake and package condition. Any unqualified product will be rejected and will not enter delivery process.

Kisspeptin-10 adopts standard crimp-top amber glass vial packaging, single vial specification:5mg,10mg. Amber glass material can block ultraviolet rays, reduce photo-oxidation of tryptophan residue in peptide sequence. Crimp sealing structure isolates oxygen and moisture, further protect lyophilized peptide cake. Each vial is sealed tightly to avoid moisture permeation. For export shipment, vials are packed with moisture-proof desiccant and shock-proof foam materials inside carton. For long-distance cross-border delivery, cold chain packaging with temperature monitoring label can be provided to maintain low-temperature environment during transportation. The packaging design fully considers peptide’s light-sensitive and hygroscopic characteristics, minimizing the impact of external light, moisture and mechanical collision during international logistics.

Unopened lyophilized Kisspeptin-10 long-term storage requirement: -20℃, dry environment, protected from light, avoid repeated freeze-thaw cycles. It is strongly recommended to equilibrate sealed vial to room temperature before opening, to prevent water condensation forming inside vial, because condensed water will cause peptide moisture absorption and accelerate degradation. After reconstitution by aqueous solvent, peptide solution stability decreases obviously, especially tryptophan residue is easy to oxidize and asparagine residue may undergo deamidation in aqueous solution. Reconstituted solution should be stored at 2~8℃, and subpackage into small aliquots is strongly recommended to minimize freeze-thaw cycles and reduce oxidation risk. Proper storage and handling can maximize retention of product quality and stability, extend usable time of peptide sample in laboratory work.

We provide complete technical guidance about product storage, sample handling and peptide reconstitution operation. Batch COA documents can be supplied as required. Our technical support team offers timely communication and professional reply for product technical inquiries. We strictly follow international export inspection requirements. Once shipment is finished, logistics tracking information can be provided for logistics monitoring. If customers encounter problems in sample storage and reconstitution during laboratory research, our team can provide professional suggestions and operational notes to help researchers protect peptide quality.