Cardiac Repair After Myocardial Infarction
Preclinical studies using mouse models of myocardial infarction have shown that thymosin β4 reduces infarct size, improves left ventricular systolic function, and stimulates the formation of new blood vessels within injured cardiac tissue. These effects are attributed to the activation of cardiac progenitor cells and the anti-apoptotic properties of the peptide.

Accelerated Skin Wound Healing
In animal models, thymosin β4 accelerates skin wound healing by stimulating the migration of keratinocytes and endothelial cells, enhancing angiogenesis, and promoting the organized deposition of collagen. Research involving chronic wound models (such as those in diabetic subjects) has demonstrated a significant improvement in the time required for wound closure.

Neuroprotection and Neuroregeneration
Studies on models of traumatic brain injury and stroke have indicated that thymosin β4 reduces neuronal inflammation, promotes the survival of oligodendrocytes, and stimulates remyelination. In animal models of central nervous system injury, the peptide has been shown to improve functional neurological scores.

Repair of Corneal Injuries
One of the most clinically advanced applications of thymosin β4 involves the healing of corneal wounds. Phase II clinical trials (RGN-259) have evaluated the efficacy of topically administered thymosin β4 for the treatment of dry eye disease and neurotrophic corneal injuries, yielding encouraging results.

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