Nystose

White crystal

The Hex4perAc of 100mg was dissolved in 5mL of ΜeOΗ, then add 18mg, 0.3eq of CH3Ona. After stirring for 2.5 h in the greenhouse, Then add Dowex50Wx2 [H+] to neutralize. After filtering the resin, the solvent is removed under reduced pressure. The remaining portion was dissolved in H2O, Desalting by (Bio-Gel P-2Gel: 10 mL). After freeze drying, a white solid (57 mg) was obtained.The hydrolytic activity of β-fructofuranosidase was assayed using sucrose as a substrate, and was monitored by measuring the amount of reducing sugar released from sucrose. The enzyme solution (50 μL) was added to 50 μL of 0.4percent sucrose in 20 mM Na citrate buffer (pH 5.5) and the mixture was incubated at 30 °C. After incubation, the enzymatic reaction was stopped by heating the sample at 95 °C for 5 min. The amount of reducing sugar in the reaction mixture was determined by the Somogyi-Nelson method using glucose as a standard.General procedure: FOS production was carried out by adding 1ml of enzyme samples collected at various time intervals to 3ml of 50percent (w/v) sucrose dissolved in 0.1M citrate buffer (pH 5.5) for period of 24h at 55°C. The amount of FOS formation in the samples was analyzed by high performance liquid chromatography (HPLC, Waters) with sugar-pak column (6.5×300mm) and refractive index (RI) differential detector (RI 2414).General procedure: FOS production was carried out by adding 1ml of enzyme samples collected at various time intervals to 3ml of 50percent (w/v) sucrose dissolved in 0.1M citrate buffer (pH 5.5) for period of 24h at 55°C. The amount of FOS formation in the samples was analyzed by high performance liquid chromatography (HPLC, Waters) with sugar-pak column (6.5×300mm) and refractive index (RI) differential detector (RI 2414).The Hex4perAc of 100mg was dissolved in 5mL of ΜeOΗ, then add 18mg, 0.3eq of CH3Ona. After stirring for 2.5 h in the greenhouse, Then add Dowex50Wx2 [H+] to neutralize. After filtering the resin, the solvent is removed under reduced pressure. The remaining portion was dissolved in H2O, Desalting by (Bio-Gel P-2Gel: 10 mL). After freeze drying, a white solid (57 mg) was obtained.The hydrolytic activity of β-fructofuranosidase was assayed using sucrose as a substrate, and was monitored by measuring the amount of reducing sugar released from sucrose. The enzyme solution (50 μL) was added to 50 μL of 0.4percent sucrose in 20 mM Na citrate buffer (pH 5.5) and the mixture was incubated at 30 °C. After incubation, the enzymatic reaction was stopped by heating the sample at 95 °C for 5 min. The amount of reducing sugar in the reaction mixture was determined by the Somogyi-Nelson method using glucose as a standard.General procedure: FOS production was carried out by adding 1ml of enzyme samples collected at various time intervals to 3ml of 50percent (w/v) sucrose dissolved in 0.1M citrate buffer (pH 5.5) for period of 24h at 55°C. The amount of FOS formation in the samples was analyzed by high performance liquid chromatography (HPLC, Waters) with sugar-pak column (6.5×300mm) and refractive index (RI) differential detector (RI 2414).General procedure: FOS production was carried out by adding 1ml of enzyme samples collected at various time intervals to 3ml of 50percent (w/v) sucrose dissolved in 0.1M citrate buffer (pH 5.5) for period of 24h at 55°C. The amount of FOS formation in the samples was analyzed by high performance liquid chromatography (HPLC, Waters) with sugar-pak column (6.5×300mm) and refractive index (RI) differential detector (RI 2414).

Molecular Weight:666.6
XLogP3:-7.4
Hydrogen Bond Donor Count:14
Hydrogen Bond Acceptor Count:21
Rotatable Bond Count:13
Exact Mass:666.22185834
Monoisotopic Mass:666.22185834
Topological Polar Surface Area:348
Heavy Atom Count:45
Complexity:964
Defined Atom Stereocenter Count:17
Covalently-Bonded Unit Count:1
Compound Is Canonicalized:Yes