5-Bromo-4-chloro-3-indolyl-beta-D-galactoside
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5-Bromo-4-chloro-3-indolyl-beta-D-galactoside
structure -
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CAS No:
7240-90-6
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Formula:
C14H15BrClNO6
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Chemical Name:
5-Bromo-4-chloro-3-indolyl-beta-D-galactoside
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Synonyms:
X-BETA-D-GAL;X-BETA-D-GALACTOSIDE;X-BETA-GAL;X-GAL READY TO USE;X-GAL;5-BROMO-4-CHLORO-1H-INDOL-3-YL BETA-D-GALACTOPYRANOSIDE;5-BROMO-4-CHLORO-1H-INDOL-3-YL BETA-D-GALACTOSIDE-(1,5);5-BROMO-4-CHLORO-3-INDOLYL-B B-D-GALACTOPYRANOSIDE
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CAS No:
Description
X-GAL(BCIG) is an organic compound consisting of galactose linked to a substituted indole; X-gal is much used in molecular biology to test for the presence of an enzyme, β-galactosidase.
5-bromo-4-chloro-3-indolyl beta-D-galactoside is an indolyl carbohydrate that is the beta-D-galactoside of 3-hydroxy-1H-indole in which the indole moiety is substituted at positions 4 and 5 by chlorine and bromine, respectively. It is used to test for the presence of an enzyme, beta-galactosidase, which cleaved the glycosidic bond to give 5-bromo-4-chloro-3-hydroxy-1H-indole, which immediately dimerises to give an intensely blue product. It has a role as a chromogenic compound. It is an indolyl carbohydrate, a beta-D-galactoside, an organochlorine compound, an organobromine compound and a D-aldohexose derivative.
White Powder
5-Bromo-4-chloro-3-indolyl-beta-D-galactoside Basic Attributes
408.63
408.63
1402009
230-640-8
V595OG374W
White
29389090
Characteristics
115
0.62
White Powder
1.5563 (rough estimate)
230 °C
673.9±55.0 °C(Predicted)
361.3±0.0 °C
1.6110 (estimate)
soluble in 100 mM in DMSO.
2-8°C
-64 º (C=1, H2O/DMF (1/1))
Odorless
12.74±0.70(Predicted)
-20°C
Safety Information
IRRITANT
NONH for all modes of transport
3
Xi
24/25-37/39-26
Xi
+4°C
Stable. Incompatible with strong oxidizing agents.
P261, P264, P270, P271, P280, P301+P312, P302+P352, P304+P312, P304+P340, P312, P322, P330, P363, P501
36/37/38
|Warning|H302 (100%): Harmful if swallowed [Warning Acute toxicity, oral]|P261, P264, P270, P271, P280, P301+P312, P302+P352, P304+P312, P304+P340, P312, P322, P330, P363, and P501|Aggregated GHS information provided by 10 companies from 3 notifications to the ECHA C&L Inventory. Each notification may be associated with multiple companies.
Drug Information
Colorless, endogenous or exogenous pigment precursors that may be transformed by biological mechanisms into colored compounds; used in biochemical assays and in diagnosis as indicators, especially in the form of enzyme substrates. Synonym: chromogens (not to be confused with pigment-synthesizing bacteria also called chromogens). (See all compounds classified as Chromogenic Compounds.)
5-bromo-4-chloro-3-indolyl beta-galactoside
5-Bromo-4-chloro-3-indolyl-beta-D-galactoside Use and Manufacturing
In histochemical staining, in blue-white selection of bacterial colonies with lacZ gene activity during cloning experiments.
A substrate for β-Galactosidase that is converted by the enzyme into an intense indigo-blue chromophore (615 nm).
magenta b-galactosidase substrate
Computed Properties
Molecular Weight:408.63
XLogP3:1.2
Hydrogen Bond Donor Count:5
Hydrogen Bond Acceptor Count:6
Rotatable Bond Count:3
Exact Mass:406.97713
Monoisotopic Mass:406.97713
Topological Polar Surface Area:115
Heavy Atom Count:23
Complexity:421
Defined Atom Stereocenter Count:5
Covalently-Bonded Unit Count:1
Compound Is Canonicalized:Yes
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5-Bromo-4-chloro-3-indolyl-beta-D-galactoside
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