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Methyl Green zinc chloride salt

Methyl Green zinc chloride salt structure

Methyl Green zinc chloride salt 

structure
  • CAS No:

    7114-03-6

  • Formula:

    C27H35BrCl3N3Zn

  • Chemical Name:

    Methyl Green zinc chloride salt

  • Synonyms:

    METHYL GREEN, ZINC CHLORIDE;METHYL GREEN, ZINC CHLORIDE SALT;LIGHT GREEN B;DOUBLE GREEN SF;CI NO 42590;CI 42590;ETHYL GREEN ZINC CHLORIDE SALT;Methyl green, crystal violet free, (

  • Categories:

    Chemical Reagents  >  Organic Reagents

Description

maroon to reddish-brown crystals


Methyl Green, Zinc chloride salt (chemical formula: C27H35Cl4N3Zn) is the zinc chloride salt form of Methyl green. Methyl green is a cationic or positive charged stain. Since it can binds selectively to the AT-rich regions of native DNA, it is mainly used for the DNA and RNA staining in the cell nuclei. Methyl Green, Zinc chloride Salt, as a CERTISTAIN® dry dye for DNA staining, it has many application: used as a tracking dye in acid buffers for electrophoresis; used to demonstrate DNA and RNA in tissues sections in the Methyl Green-Pyronin method; used as an exclusion viability stain for cells. In addition, it can also be used in fluorimetric assay and flow cytometry.


Crystallise the dye from hot water. [Beilstein 13 IV 2286.]

Methyl Green zinc chloride salt Basic Attributes

653.23

649.037109

230-415-4

RVA98Y3Y2E

Green

29215900

Characteristics

629 nm, 423 nm

6.25000

0.36490

Green Solid

>300 °C(lit.)

70 g/L (20 ºC)

Store at RT.

4.6 (20°C, 10g/L in H2O)

Store at RT.

Safety Information

NONH for all modes of transport

3

Xi,N,C

26-36-61-60-45-36/37/39

QD2160000

Xi,N,C

Stable. Incompatible with strong oxidizing agents.

P261-P305 + P351 + P338

36/37/38-50/53-34-22

|Warning|H315 (91.3%): Causes skin irritation [Warning Skin corrosion/irritation]|P261, P264, P271, P280, P302+P352, P304+P340, P305+P351+P338, P312, P321, P332+P313, P337+P313, P362, P403+P233, P405, and P501|Aggregated GHS information provided by 46 companies from 5 notifications to the ECHA C&L Inventory. Each notification may be associated with multiple companies.

Methyl Green zinc chloride salt Use and Manufacturing

Used for bacterial staining (nuclear staining of fresh specimens) methyl green-hydrochloric acid mixture for staining of sperm, gonococci and mast cells.


Methyl Green is a DNA stain used as a tracking dye in acid buffers for electrophoresis. It helps differentiate bacteria and is also a tissue stain for nucleoli and mitochondria in tissues. It is used with Bismarck Brown frequently.

Computed Properties

Molecular Weight:653.2
Hydrogen Bond Acceptor Count:5
Rotatable Bond Count:5
Exact Mass:649.03714
Monoisotopic Mass:649.03714
Topological Polar Surface Area:6.2
Heavy Atom Count:35
Complexity:645
Covalently-Bonded Unit Count:6
Compound Is Canonicalized:Yes

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