Superoxide dismutase
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Superoxide dismutase
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CAS No:
9054-89-1
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Chemical Name:
Superoxide dismutase
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Synonyms:
Superoxide dismutase;Dismutase,superoxide;E.C. 1.15.1.1;EC 1.15.1.1;Superoxide dismutase (EC 1.15.1.1);Superphycodismutase;Peroxide dismutase;Dismutin BT;SOD;Extramel;Peroxinorm;Palosein;Dismutase
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CAS No:
Characteristics
0.99
blue-gray powder
1.5±0.1 g/cm3
359.1±42.0 °C at 760 mmHg
183.5±20.7 °C
1.641
Dissolves readily at 5 mg/mL in 0.05 M potassium phosphate buffer, pH 7.8, containing 0.1 mM EDTA.
2-8°C
Superoxide dismutase Use and Manufacturing
Collect and wash fresh pig blood, centrifuge to remove yellow plasma, red blood cells are centrifugally washed with sodium chloride solution (9g/L or 0.9%), remove the washing liquid, and wash repeatedly 3 times to get clean red blood cells. Porcine blood was removed by centrifugation of fresh pig blood → NaCl was collected and washed 3 times → washed and red blood cells were hemolyzed and dehemoglobin was added to clean red blood cells at a temperature of 50C. Deionized water was added to the clean red blood cells and stirred for 30 minutes. 95%) and 15ml of chloroform, stirred for 15min, centrifuged to remove hemoglobin, and collect the supernatant. Clean red blood cells deionized water; 50; 30min → hemolysis} hemolysate ethanol; chloroform → dehaemoglobin supernatant precipitation, heat treatment at about 00C, add 1.2 to 1.5 times the volume of acetone to the supernatant, wait for a large amount of flocculent precipitation After centrifugation, the supernatant was removed to obtain a precipitate. The precipitate was dissolved with appropriate amount of deionized water, centrifuged to remove insoluble proteins, the supernatant was heat treated at 55 to 650C for 10 to 15 minutes, centrifuged to remove a large amount of heat-denatured protein, and the yellow-green supernatant was collected. Supernatant acetone; 00→precipitation precipitation deionized water; 600→heat treatment yellow-green clarification solution precipitation, insoluble protein removal, dialysis. Add appropriate amount of acetone to the supernatant at 00C to make a large amount of flocculent precipitate, centrifuge, remove The supernatant is collected, the precipitate is added, and fully stirred with deionized water to dissolve, and the insoluble protein is removed by centrifugation. The supernatant is placed in a dialysis bag and dynamically dialyzed for 6 to 8 hours to obtain dialysate. Yellow-green clear solution acetone; deionized water; 00→precipitation; insoluble protein; dialysis dialysate adsorption, elution, concentration, lyophilization. Add dialysate to ph7.6 phosphate buffer (2.5mmol/L) to equilibrate Adsorb on a good DEAE-Sephadex A-50 chromatography column, perform gradient elution with ph7.6 potassium phosphate buffer (2.5-50 mmol/L), collect the eluent with superoxide dismutase activity, and ultrafiltration , Concentrated, freeze-dried, that is, the product of superoxide dismutase dialysate DEAE-Sephadex A-50; potassium phosphate buffer; Ph7.6 → ^ adsorption, elution, ultrafiltration; concentration, freeze-dried product electrophoresis identification conditions Agarose gel (5g/L is 0.5g) plate electrophoresis, using ph8.4 tris-hydroxymethylaminomethane-glycine as buffer, voltage gradient 22V/cm, current 3mA/cm, electrophoresis time 35min, fixed staining solution 5g /L (0.5%) amino black 10B, the decolorizing solution is 7g/L (7%) acetic acid.
Superoxide dismutase from bovine erythrocytes has been used in a study to assess a kinetic model of radiation-induced inactivation of superoxide dismutase in nitrous oxide-saturated solutions. Superoxide dismutase from bovine erythrocytes has also been used in a study to investigate the possible participation of superoxide anion in the intestinal tryptophan 2,3-dioxygenase reaction.
Drug Function and Efficacy
No specific pharmacological effects are clearly mentioned from the above information
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