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Fluorescein, diacetate

Fluorescein, diacetate structure

CAS No:
596-09-8
Formula:
C24H16O7
Chemical Name:
Fluorescein, diacetate
Synonyms:
Spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one,3′,6′-bis(acetyloxy)-;Fluorescein,diacetate;3′,6′-Bis(acetyloxy)spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one;Fluorescein diacetate;3′,6′-Diacetylfluorescein;Fluorescein 3′,6′-diacetate;MFCD 5062;NSC 4726;NSC 667259;F 1303;FDA;(6′-Acetyloxy-3-oxospiro[2-benzofuran-1,9′-xanthene]-3′-yl) acetate
Categories:
Analytical Chemistry > Standard

Description

Fluorescein diacetate is a cell permeable esterase-substrate. Fluorescein diacetate can be used as a fluorogenic substrate for hGSTP1-1.

Fluorescein, diacetate Basic Attributes

Molecular Weight：

416.38

Exact Mass：

416.38

BRN：

62575

EC Number：

209-877-6

UNII：

YL39R93PRE

NSC Number：

667259|4726

DSSTox ID：

DTXSID4060490

HScode：

32129000

Characteristics

PSA：

88.13000

XLogP3：

2.77

Appearance：

Light yellow Crystalline Powder

Density：

1.5±0.1 g/cm3

Melting Point：

205-206 °C

Boiling Point：

604.7±55.0 °C at 760 mmHg

Flash Point：

264.0±31.5 °C

Refractive Index：

1.681

Water Solubility：

soluble in chloroform, DMSO, ethanol, and water.acetone: soluble 25mg/mL

Storage Conditions：

−20°C

Safety Information

Transport codes：

NONH for all modes of transport

wgk germany：

hazard class code：

36/37/38

safety instructions：

24/25-36/37/39-27-26

Marks：

Drug Information

MeSH Entry Terms：

3',6'-diacetylfluorescein

Fluorescein, diacetate Use and Manufacturing

Uses

Esterase substrate. FDA can penetrate cell membranes and accumulate in living cells as fluorescein. Since fluorescein is less hydrophilic than BCECF or Calcein, the amount of fluorescein leaking from cells is also high. FDA can also be used for flow cytometry. The excitation and emission wavelengths of fluorescein are 488 nm and 530 nm, respectively.

Manufacturing Info：

Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-bis(acetyloxy)-: ACTIVE

Computed Properties

Molecular Weight:416.4
XLogP3:3.6
Hydrogen Bond Acceptor Count:7
Rotatable Bond Count:4
Exact Mass:416.08960285
Monoisotopic Mass:416.08960285
Topological Polar Surface Area:88.1
Heavy Atom Count:31
Complexity:708
Covalently-Bonded Unit Count:1
Compound Is Canonicalized:Yes

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