Sodium cholate

Cholic acid sodium is a major primary bile acid produced in the liver and usually conjugated with glycine or taurine. It facilitates fat absorption and cholesterol excretion.

Sodium cholate is a cholate salt and an organic sodium salt.|A trihydroxy bile salt that is used as a digestive aid in dietary supplements. It is used in culture media and in conjunction with PAPAIN and PANCREATIN.

Method 1. Preparation of crude bile acid by acid precipitation method Take bovine (or sheep, pig) bile and heat and concentrate to half, add 1-2 times the volume of 95% ethanol while it is hot, reflux and extract for a few minutes to obtain the extract. The extract is cooled, filtered, and the filtrate is distilled to recover ethanol until the content is foamed to obtain an extract. Add dilute hydrochloric acid to the extract, adjust the pH to 3-4, leave it overnight, pour out the supernatant, and the precipitate is the crude bile acid. Cow (or sheep, pig) bile [95% ethanol] → [heat reflux extraction] extract [filter] → [distillation] extract [pH3-4] → crude bile acid or take fresh or frozen chicken (or duck, goose) ) Bile, add 1/10 times the amount of industrial sodium hydroxide, heat and boil for 20-24 hours, constantly replenish the amount of evaporated water, cool, add an equal volume of hydrochloric acid, adjust the pH 2-3, a black paste appears, take it out, and wash until nearly Neutral, with total bile acid. Chicken (goose, duck) bile [NaOH, boiling] → saponification solution [HCl, pH2] → preparation of total bile acid bile acid Wash the crude bile acid with a small amount of acid water for 1-2 times, add 95% ethanol to heat to dissolve, add alkali Adjust pH 8-8.5, add activated carbon to decolorize, filter, distill the filtrate to recover ethanol, and dry to obtain bile acid powder. Crude bile acid [acid water]→[95% ethanol]→[heating][pH8-8.5, activated carbon]→bile acid method two, ethanol extraction method bile paste pretreatment Take bile paste and add its quality 1/10-1/5 Activated carbon, heated, fully stirred, steamed to remove the water until the solid matter can be crushed, it is necessary to treat the bile paste. Bile paste [activated carbon] → Preparation of crude bile salt for treatment of bile paste. Take the pretreated bile paste, add 95% ethanol to extract 3-5 times, combine the filtrate, distill the filtrate to recover the ethanol, and evaporate to dryness to obtain a light yellow bile salt Crude. Treatment of bile paste [95% ethanol] → filtrate [recovered ethanol] → crude bile salt. Method 3. Salting out method. Preparation of crude bile salt. Take bile and add ethanol to remove protein. Steam the residual liquid to remove ethanol and add 100-200g/L Sodium chloride, placed at low temperature for a few days, precipitated most of the bile salts, filtered, and obtained crude bile salts. Bile [NaCl]→[Leave for a few days at low temperature] Preparation of crude bile salt products. Take the crude bile salt, wash it twice with saturated sodium chloride aqueous solution, dry, crush, add 95% ethanol for heating and extract, add activated carbon Decolorize, evaporate to dryness, prepare bile acid salt boutique. Crude bile acid salt [washed]→[dry, crushed][95% ethanol extraction]→[decolorized, evaporated to dry] Bile salt boutique method 4. The synthesis of primary bile acid with cholesterol as raw material The role of cholesterol in multiple enzymes After a series of catabolism, combined primary bile acids (cholic acid and chenodeoxycholic acid) are finally formed. Synthesis of secondary bile acids After the primary bile acids enter the small intestine with bile and participate in the digestion and absorption of lipids, most of them are reabsorbed in the portal venous blood circulation without change. About 1/4 of the combined primary bile acids in the jejunum, ileum and upper colon are catalyzed by bacterial enzymes to further generate deoxycholic acid and lithocholic acid. Secondary bile acids (in the process of synthesizing secondary bile acids) , Can produce a small amount of ursodeoxycholic acid). Synthesis of combined bile acid The bile acid synthesized by hepatocytes combines with glycine or taurine to form a combined bile acid, which is secreted into the human biliary tract.

Biochemical reagent, anionic protein detergent.

Molecular Weight:430.6
Hydrogen Bond Donor Count:3
Hydrogen Bond Acceptor Count:5
Rotatable Bond Count:4
Exact Mass:430.26951862
Monoisotopic Mass:430.26951862
Topological Polar Surface Area:101
Heavy Atom Count:30
Complexity:643
Defined Atom Stereocenter Count:11
Covalently-Bonded Unit Count:2
Compound Is Canonicalized:Yes