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Ethidium

Ethidium structure

Ethidium 

structure
  • CAS No:

    3546-21-2

  • Formula:

    C21H20N3

  • Chemical Name:

    Ethidium

  • Synonyms:

    Phenanthridinium,3,8-diamino-5-ethyl-6-phenyl-;Phenanthridinium compound,3,8-diamino-5-ethyl-6-phenyl-;3,8-Diamino-5-ethyl-6-phenylphenanthridinium;Ethidium;Homidium;Ethidium cation

Description

ChEBI: The fluorescent compound widely used in experimental cell biology and biochemistry to reveal double-stranded DNA and RNA.


Ethidium is the fluorescent compound widely used in experimental cell biology and biochemistry to reveal double-stranded DNA and RNA. It has a role as an intercalator and a fluorochrome. It derives from a hydride of a phenanthridine.|A trypanocidal agent and possible antiviral agent that is widely used in experimental cell biology and biochemistry. Ethidium has several experimentally useful properties including binding to nucleic acids, noncompetitive inhibition of nicotinic acetylcholine receptors, and fluorescence among others. It is most commonly used as the bromide.

Ethidium Basic Attributes

314.412

314.166

EN464416SI

DTXSID9048487

Characteristics

55.9

4.2

Drug Information

Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant and Hackh's Chemical Dictionary, 5th ed, p301, p499) (See all compounds classified as Indicators and Reagents.)|Chemicals that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags. (See all compounds classified as Fluorescent Dyes.)|Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction. (See all compounds classified as Enzyme Inhibitors.)|Drugs that bind to nicotinic cholinergic receptors (RECEPTORS, NICOTINIC) and block the actions of acetylcholine or cholinergic agonists. Nicotinic antagonists block synaptic transmission at autonomic ganglia, the skeletal neuromuscular junction, and at central nervous system nicotinic synapses. (See all compounds classified as Nicotinic Antagonists.)|Agents destructive to the protozoal organisms belonging to the suborder TRYPANOSOMATINA. (See all compounds classified as Trypanocidal Agents.)

Bromide, Ethidium

Computed Properties

Molecular Weight:314.4
XLogP3:4.2
Hydrogen Bond Donor Count:2
Hydrogen Bond Acceptor Count:2
Rotatable Bond Count:2
Exact Mass:314.165722651
Monoisotopic Mass:314.165722651
Topological Polar Surface Area:55.9
Heavy Atom Count:24
Formal Charge:1
Complexity:419
Covalently-Bonded Unit Count:1
Compound Is Canonicalized:Yes

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