Hemin

Dark purple crystalline powder

Chloro(7,12-diethenyl-3,8,13,17-tetramethyl-21H,23H-porphine-2,18-dipropanoato(4-)-N(21),N(22),N(23),N(24)) ferrate(2-) dihydrogen.

Heme preparation methods include base-organic solvent method, glacial acetic acid method, acetone method, methanol or methanol-ethanol method, ion exchange cellulose method. Method one: Base-organic solvent method, take 60L methanol and 1kg diethylamine into the reaction tank, stir evenly, add 5kg fresh bovine blood powder, stir and extract at 45℃ for 1h, cool, filter, and concentrate the filtrate to 5L, add ice Acetic acid 10L, strontium chloride 200g, heating to remove residual methanol, heating to 100-102 ℃ for 1h, cooling, filtering, collecting hemin chloride crystals. The hemin chloride crystals were washed sequentially with glacial acetic acid, water, and acetone, and dried to obtain 85g hemin chloride. Bovine blood powder [methanol, diethylamine] → [45°C, 1h] [filtration] → filtrate [ice Hac, SrCl2] → [100-102°C, 1h] [filtration, washing, drying] → take hemoglobin 60L Methanol and 5kg spray-dried bovine erythrocytes were added to the reaction tank, stirred evenly, extracted at 40°C for 30min, cooled, filtered, and the filtrate was concentrated to 5L, added with 10L of glacial acetic acid, 100g of sodium chloride, dissolved with 2L of water, heated to remove residues Methanol, heated to 100 ℃ for 1h, cooled, filtered, collected hemin chloride crystals. The hemin chloride crystals were washed sequentially with glacial acetic acid, water, and dried to obtain heme chloride 65g. Bovine erythrocytes [methanol] → [40°C, 30min] [filtration] → filtrate [ice Hac, NaCl, H2O] → [100°C, 1h] [filtration, washing, drying] → preparation of heme hemoglobin fresh 300kg of blood powder, 1200L of methanol, 30kg of dichloroacetic acid, stir evenly, extract with stirring at 45℃ for 1h, cool, filter, and concentrate the filtrate to dryness to obtain 45kg of heme. Fresh blood powder [methanol, dichloroacetic acid] → [45°C, 1h] [filtration, concentration] → Preparation of heme hemoglobin Take heme 45kg, add 100L of glacial acetic acid containing 2kg NaCl, extract by boiling reflux for 1h, cool , Filtered to obtain heme chloride, washed with glacial acetic acid, water, and dried to obtain 4.8kg heme chloride. Heme [mixed solvent of NaCl and glacial acetic acid] → [boiling for 1h] [washing and drying] → heme chloride Method II. Preparation of acidic blood powder by methanol-ethanol mixed solvent 100kg blood powder is added, plus 100kg of 0.6mol/L HCl solution , Stir hemolysis and spray dry to obtain 40kg of acid blood powder. Blood powder [HCl]→Preparation of acidic blood powder heme Take 40kg of acidic blood powder, extract three times with a mixed solvent consisting of 90% ethanol, 6% methanol and 4% water. The amount of mixed solvent is 800L, 300L and 200L respectively, and the supernatant is combined Liquid, concentrated by distillation to produce heme. Acid blood powder [mixed solvent] → [extract 3 times] supernatant [concentrate] → heme.

In the food industry, heme can replace the coloring agent nitrite and synthetic pigments in meat products; in the pharmaceutical industry, heme can be used as a raw material for semi-synthetic bilirubin, and can be used to prepare anti-cancer drugs; Clinically, heme can be made into heme iron supplements. Nutritional supplements (iron fortifiers), extracted from pig blood, recognized by modern medicine to prevent and treat iron deficiency anemia, with high absorption rate, good effect, lower biological iron source, no iron smell, and no gastrointestinal irritation . It is the first choice for the production of natural iron-supplemented foods, medicines, health products, and cosmetics. Biochemical research; used as a blood test stain; used in the preparation of hematoporphyrin hydrochloride

Molecular Weight:651.9
Hydrogen Bond Donor Count:2
Hydrogen Bond Acceptor Count:9
Rotatable Bond Count:8
Exact Mass:651.146144
Monoisotopic Mass:651.146144
Topological Polar Surface Area:102
Heavy Atom Count:44
Complexity:1010
Covalently-Bonded Unit Count:3
Compound Is Canonicalized:Yes