The use of biological buffer HEPES in the kit

The chemical name of the biological buffer HEPES product is: 4-hydroxyethylpiperazine ethanesulfonic acid, a white crystalline powder. It is a weak acid and a zwitterionic buffer in organic chemicals. The decomposition ability of the substance can increase with the increase of temperature, and decrease with the decrease of temperature. However, compared with other buffers, its decomposition constant does not change much with temperature. This makes HEPES buffers more effective at low temperatures. A buffer to keep the structure and function of the enzyme well. It is widely used in many fields such as clinical laboratory diagnostic reagents, life science research, pharmacy and biotechnology.

1) The research of biological buffer HEPES on promoting the stability of foot-and-mouth disease antigen.

Foot-and-mouth disease antigen is easily degraded during storage and vaccine preparation, which affects the vaccine effect. The degradation rate of the foot-and-mouth disease antigen is related to the stability of the pH value of the maintenance solution. The preparation of a maintenance solution with strong pH stability can improve the quality of the foot-and-mouth disease vaccine.

2) Preparation of a skin care product containing a biological buffer, which contains a microbial fermentation product extract, which is characterized in that it also contains a biological buffer with a mass percentage of 0.1%-3.0%. Advantages: The pH value of the product has a small drift range. Within three years after the preparation of the skin care product, the pH value can be stabilized at 6.0-8.0, which has a good protection for the microbial fermentation product extract added therein, and the microbial fermentation product extract is not Easily hydrolyzed, the anti-aging effect of the product lasts for a long time.

3) Prepare a kit for detecting the content of 25-hydroxyvitamin D3.

This kit has high detection sensitivity, strong specificity, accurate quantification and wide linear range. It is especially suitable for the detection of samples with very low 25-hydroxyvitamin D3 content. In addition, the kit of the present invention does not need to pre-dilute the sample during the detection process, which is convenient for clinical use, simple operation, low detection cost, and is suitable for various automatic biochemical analyzers.

4) Preparation of lactate dehydrogenase detection kit.

This detection kit adopts dual reagent mode to effectively avoid the interference of non-specific reactions, and at the same time minimize the interference from the turbidity of the sample itself, to ensure the stability and reliability of the measurement results, with good stability, high accuracy, and linear test range Wide, good repeatability, strong anti-interference and other advantages. In addition, the detection kit of the present invention does not need to pre-dilute the sample during the detection process, which is convenient for clinical use, simple and fast to operate, and is suitable for a fully automatic biochemical analyzer.

5) Prepare a neuron-specific enolase content detection kit, which belongs to the field of human neuron-specific enolase content detection.

This detection kit has high sensitivity and a wide detection linear range, and has the advantages of simple operation, rapidity, strong specificity, good stability and high sensitivity.

In addition to HEPES, the biological buffers produced by Desheng include Tris, Bicine, Caps, Mops, Taps, EPPS, etc.; they are mainly used in biochemical diagnostic kits, DNA/RNA extraction kits and PCR diagnostic kits. Customers in need can provide samples.