Tris full name: trimethylol aminomethane, as a commonly used chemical, has a very wide range of applications in various fields. For example, Tris can be seen in the fields of medicine, daily chemical products, special materials and so on, and there is an application in it, "nucleic acid purification". The purification of nucleic acid needs saturated phenol, Tris also has saturated phenol, also known as basic phenol and Tris equilibrium phenol. Tris saturated phenol is a light yellow transparent liquid saturated with tris clph8.0, with pungent odor. The upper layer is Tris hydrochloric acid buffer, and the solution is added with antioxidant 8-hydroxyquinoline. It is suitable for removing protein and extracting DNA from nucleic acid samples. Although there are ready-made products on the market, it is better to master the configuration method of Tris saturated phenol. The detailed configuration steps of Tris saturated phenol are described below.
Tris saturated phenol:
1. Take out the re steamed phenol from the refrigerator and place it in a 68 ℃ water bath at room temperature to dissolve it. Do not put it in a 68 ℃ water bath immediately to prevent the glass from cracking;
2. Add 8-hydroxyquinoline to 0.1% and β - mercaptoethanol to 0.2% (stock solution 14.4mol/ml), mix well, the solution turns yellow, and pour into the separating funnel (it can also be carried out in a beaker);
3. Add 1mol / ml tris (ph8.0) of the same solvent, mix it repeatedly, and let it stand still until it is layered; release the Yellow phenol solution in the lower layer and discard the upper layer;
4. Add solid Tris about 1g / 100ml phenol, shake well and remove the aqueous phase;
5. Add 0.1mol/mltris (ph8.0) to balance several times until pH is 8.0;
6. Add 0.1mol/mltris (ph8.0) and store in brown bottle for 4 degrees;
7. If yellow disappears or pink, it cannot be used.
Tris saturated phenol is toxic and has a strong irritant effect on skin and human body, so it needs to be operated in the fume hood. Avoid harm, safety first.
The above is the configuration method of Tris saturated phenol provided by Hubei xindesheng Material Co., Ltd. In addition to Tris, Desheng also produces other chemicals, such as vascular additives, enzymes, chemiluminescence reagents, biological buffers, chromogen substrates and carbomer. At present, Desheng company has been established for decades, has its own R & D team, and has invested a lot of energy in biological buffers. Reliable product quality, price concessions can be discussed, interested in understanding, you can call consultation, Desheng welcome your call.
