The weakness of Tris buffer

Tris chemical name: trihydroxymethyl aminomethane, which is the representative of "C" position in our biological buffer. In addition to being used as buffer in biochemistry and molecular biology experiments, it is also used as pH regulator in surfactant, cosmetics and virus preservation solution, vulcanization accelerator and pharmaceutical intermediates, etc. it can be said that it is one of the more widely used biological buffers. Just for these purposes, let's give it a thumbs up. What's more, we can also prepare other buffer products of the same series through Tris. However, although it is so "powerful", it is actually "fragile".

Appearance - yellowing

The possibility of Tris yellowing caused by product quality problems is not great, because the raw materials and solvents used in the synthesis process are colorless. If the raw materials used are of poor quality, contain colored impurities or the equipment is not cleaned and the product is polluted, then the problem of Tris yellowing may occur.

Heart - non toxic

Tris is widely used, from biochemical experiments in the laboratory, to large-scale industrial production, and then to daily necessities. When it comes to its toxicity, in fact, regardless of the dose of any substance, the toxicity belongs to rogue. There are three molecules of formaldehyde hidden in the tris molecule, The problem is not very big, but how to inhale for a long time and absorb through the skin will have some impact on the body. Therefore, it is an effective protective measure for the on-site laboratory personnel to wear gloves and goggles during operation, which can be used safely in production.

Temperature changes it

The temperature effect is large, and the temperature change has a great influence on the pH value of the buffer solution. For example, the pH value of the buffer solution at 4 ℃ is 8.4, and the pH value at 37 ℃ is 7.4. Therefore, it must be prepared at the service temperature. The tris HCl buffer solution prepared at room temperature cannot be used at 0 ℃ ~ 4 ℃.