Native Escherichia coli Chloramphenicol Acetyltransferase
9040-07-7
2026-09-30
Coenzymes,Inhibito,Enzymes,Zymogens,Substrates,Native Microorganism Creatine Amidinohydrolase
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Product Description
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Seller Information
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Description
ProductName Native Escherichia coli Chloramphenicol Acetyltransferase Cat No NATE-0107 Description Chloramphenicol acetyltransferase (or CAT) is a bacterial enzyme (EC 2.3.1.28) that detoxifies the antibiotic chloramphenicol and is responsible for chloramphenicol resistance in bacteria. This enzyme covalently attaches an acetyl group from acetyl-CoA to chloramphenicol, which prevents chloramphenicol from binding to ribosomes. A histidine residue, located in the C-terminal section of the enzyme, plays a central role in its catalytic mechanism. Abbr CAT, Native (E. coli) Alias CAT; CAT I; CAT II; CAT III Source Escherichia coli Applications Chloramphenicol acetyltransferase from Escherichia coli has been used in a study to assess the construction of a novel expression system in Klebsiella pneumoniae and its application for 1,3-propanediol production. Chloramphenicol acetyltransferase from Escherichia coli has also been used in a study to investigate site-directed mutagenesis and promoter functional analysis of the RM07 DNA fragment from Halobacterium halobium. The enzyme has been used in chloramphenicol acetyltransferase assay to optimize the transfection of plasmid DNA into primary cultures of adult mouse keratinocytes. It has also been used to assess the acetyl-CoA carboxylase-carboxyltransferase (ACC-CT) domain activity. This has been done using a coupled-two phase system measuring the selective partition of [14C]acetylchloramphenicol into an organic layer. Form Type I, lyophilized powder. Partially purified; contains Tris buffer salts; Type II, buffered aqueous glycerol solution. Clear, colorless solution in 50% glycerol containing 5 mM Tris-HCl, pH 7.8, and 0.5 mM 2-mercaptoethanol Activity 50,000-150,000 units/mg protein (Lowry) CAS No 9040-07-7 Molecular Weight mol wt 75 kDa (three identical subunits) Unit Definition One unit will convert 1.0 nanomole of chloramphenicol and acetyl-CoA to chloramphenicol 3-acetate and CoA per min at pH 7.8 at 25°C. Optimum pH 7.8 Storage −20°C Synonyms Acetyl-CoA:chloramphenicol 3-O-acetyltransferase; CAT; 9040-07-7; chloramphenicol acetyltransferase; chloramphenicol acetylase; chloramphenicol transacetylase; CAT I; CAT II; CAT III category Specialty Chemicals(c26) cas_num 9040-07-7 Basic Info-
Product Name:
CHLORAMPHENICOLACETYLTRANSFERASE
Other Name:EC 2.3.1.28;CHLORAMPHENICOL ACETYLTRANFERASE;CHLORAMPHENICOL ACETYLTRANSFERASE;chloramphenicol acetyltransferase*from escherichi;ACETYL-COA: CHLORAMPHENICOL 3-O-ACETYLTRANSFERASE;CHLORAMPHENICOL ACETYLTRANSFERASEFROM ES CHERICHIA;CHLORAMPHENICOL ACETYLTRANSFERASEGLYCERO L SOLUTION;chloramphenicol acetyltransferase from escherichia coli;Acetyl-CoA: chloramphenicol 3-O-acetyltransferase, CAT
CAS No.:9040-07-7
Molecular Formula:C11H12Cl2N2O5
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Hazard IdentificationClassification of the substance or mixture
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no data available
Handling and StoragePrecautions for safe handling
Handling in a well ventilated place. Wear suitable protective clothing. Avoid contact with skin and eyes. Avoid formation of dust and aerosols. Use non-sparking tools. Prevent fire caused by electrostatic discharge steam.
Conditions for safe storage, including any incompatibilities
Store the container tightly closed in a dry, cool and well-ventilated place. Store apart from foodstuff containers or incompatible materials.
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Seller Information
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Business Type:
Manufactory
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Main Products:
Coenzymes,Inhibito,Enzymes,Zymogens,Substrates,Native Microorganism Creatine Amidinohydrolase
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Location:
Shirley, New York 11967, USA
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Payment Terms:
TT against copy of documents,D/P
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Average lead Time:
15
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Total Annual Revenue:
$1 million-$2.5 million
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Year of Establishment:
2004
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