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o-Phenylenediamine dihydrochloride

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CAS No.:

615-28-1

Price valid (until):

2026-08-04

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Creative Enzymes

Company Type:

Manufactory

Location:

United States

Qualification:

Main Products:

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Product Description
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Description

ProductName	o-Phenylenediamine dihydrochloride
Cat No	CSUB-0420
Applications	o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
Form	tablet
CAS No	615-28-1
Molecular Weight	181.06
Storage	2-8°C
MDL Number	MFCD00012966
PubChem Substance ID	24898979.0
Quality Level	PREMIUM
mp	258 °C (dec.)(lit.)
Reconstitution	Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.
Beilstein Registry Number	3912045.0
Substrates	Peroxidase Substrates
category	Amides(c1311)
cas_num	615-28-1

Basic Info

Product Name:

o-Phenylenediamine, dihydrochloride

Other Name:

1,2-Benzenediamine,hydrochloride (1:2);o-Phenylenediamine,dihydrochloride;1,2-Benzenediamine,dihydrochloride;1,2-Phenylenediamine dihydrochloride;1,2-Diaminobenzene dihydrochloride

CAS No.:

615-28-1

Molecular Formula:

C6H8N2.2ClH

InChIKeys:

InChIKey=RIIWUGSYXOBDMC-UHFFFAOYSA-N

Molecular Weight:

181.06

Exact Mass:

180.02200

EC Number:

210-418-7

UNII:

Z16252Z50J

UN Number:

2811|1673

DSSTox ID:

DTXSID3021140

Color/Form:

Needles

HScode:

2921590090

Categories:

Amides

Characteristics

PSA:

52.04000

XLogP3:

3.61740

Appearance:

white to off-white tablet

Melting Point:

200 °C (decomp) @ Solvent: Water

Boiling Point:

257ºC at 760 mmHg

Flash Point:

124.9ºC

Water Solubility:

Slightly soluble in water; freely soluble in alcohol, chloroform or ether

Storage Conditions:

2-8°C

Toxicity:

Peritoneal-rat LD50: 290 mg/kg; peritoneal-mouse LD50: 200 mg/kg

Flammability characteristics:

Open flame is flammable; heat releases toxic hydrogen chloride and nitrogen oxide gas

Experimental Properties:

When heated to decomposition it emits very toxic fumes of HCl and NOx

Air and Water Reactions:

Slightly soluble in water.

Reactive Group:

Salts, Acidic

Reactivity Profile:

Acidic organic/inorganic salts, such as 1,2-PHENYLENEDIAMINE DIHYDROCHLORIDE, are generally soluble in water. The resulting solutions contain moderate to high concentrations of hydrogen ions and have pH's of less than 7.0. They react as acids to neutralize bases. These neutralizations generate heat, but less or far less than is generated by neutralization of inorganic acids, inorganic oxoacids, and carboxylic acid. They usually do not react as either oxidizing agents or reducing agents but such behavior is not impossible. Many of these compounds catalyze organic reactions.

See More o-Phenylenediamine, dihydrochloride Properties 

Hazard Identification

Classification of the substance or mixture

Acute toxicity - Category 3, Oral

Acute toxicity - Category 4, Dermal

Eye irritation, Category 2

Skin sensitization, Category 1

Acute toxicity - Category 4, Inhalation

Germ cell mutagenicity, Category 2

Carcinogenicity, Category 2

Hazardous to the aquatic environment, short-term (Acute) - Category Acute 1

Hazardous to the aquatic environment, long-term (Chronic) - Category Chronic 1

GHS label elements, including precautionary statements

Precautionary statement(s)
Pictogram(s)
Signal word	Danger
Hazard statement(s)	H301 Toxic if swallowed H312 Harmful in contact with skin H319 Causes serious eye irritation H317 May cause an allergic skin reaction H332 Harmful if inhaled H341 Suspected of causing genetic defects H351 Suspected of causing cancer H410 Very toxic to aquatic life with long lasting effects
Prevention	P264 Wash ... thoroughly after handling. P270 Do not eat, drink or smoke when using this product. P280 Wear protective gloves/protective clothing/eye protection/face protection/hearing protection/... P261 Avoid breathing dust/fume/gas/mist/vapours/spray. P272 Contaminated work clothing should not be allowed out of the workplace. P271 Use only outdoors or in a well-ventilated area. P203 Obtain, read and follow all safety instructions before use. P273 Avoid release to the environment.
Response	P301+P316 IF SWALLOWED: Get emergency medical help immediately. P321 Specific treatment (see ... on this label). P330 Rinse mouth. P302+P352 IF ON SKIN: Wash with plenty of water/... P317 Get medical help. P362+P364 Take off contaminated clothing and wash it before reuse. P305+P351+P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P333+P317 If skin irritation or rash occurs: Get medical help. P304+P340 IF INHALED: Remove person to fresh air and keep comfortable for breathing. P318 IF exposed or concerned, get medical advice. P391 Collect spillage.
Storage	P405 Store locked up.
Disposal	P501 Dispose of contents/container to an appropriate treatment and disposal facility in accordance with applicable laws and regulations, and product characteristics at time of disposal.

Other hazards which do not result in classification

no data available

Handling and Storage

Precautions for safe handling

Handling in a well ventilated place. Wear suitable protective clothing. Avoid contact with skin and eyes. Avoid formation of dust and aerosols. Use non-sparking tools. Prevent fire caused by electrostatic discharge steam.

Conditions for safe storage, including any incompatibilities

Store the container tightly closed in a dry, cool and well-ventilated place. Store apart from foodstuff containers or incompatible materials.

See More o-Phenylenediamine, dihydrochloride Safety Data Sheet 

Seller Information

Business Type:
Manufactory

Main Products:
Coenzymes,Inhibito,Enzymes,Zymogens,Substrates,Native Microorganism Creatine Amidinohydrolase

Location:
Shirley, New York 11967, USA

Payment Terms:
TT against copy of documents,D/P

Average lead Time:
15

Total Annual Revenue:
$1 million-$2.5 million

Year of Establishment:
2004

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