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3,3',5,5'-Tetramethylbenzidine dihydrochloride hydrate

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CAS No.:

207738-08-7

Sample Available:

Yes

Price valid (until):

2026-11-18

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Creative Enzymes

Company Type:

Manufactory

Location:

United States

Payment Terms:

TT against copy of documents,D/P

Average Lead Time:

15 days

Qualification:

Main Products:

Coenzymes,Inhibito,Enzymes,Zymogens,Substrates,Native Microorganism Creatine Amidinohydrolase

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Product Description
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Description

ProductName	3,3',5,5'-Tetramethylbenzidine dihydrochloride hydrate
Cat No	CSUB-0456
Applications	Noncarcinogenic substitute for benzidine.
CAS No	207738-08-7
Molecular Weight	313.27 (anhydrous basis)
Storage	2-8°C
Synonyms	TMB dihydrochloride
MDL Number	MFCD00150104
PubChem Substance ID	24900558.0
Substrates	Peroxidase Substrates
category	Biochemical Reagents(c1519)
cas_num	207738-08-7

ECHEMI Editorial Reference

Off-white fluffy powder
TMB is an aromatic amine that undergoes oxidation by the higher oxidation states of heme peroxidases (compounds I and II) thereby serving as a reducing co-substrate. One electron oxidation of TMB results in a radical cation that forms a charge-transfer complex with the unoxidized compound. This charge transfer complex absorbs at 652 nm (ε = 39,000). The completely oxidized form (diimine) absorbs at 450 nm (ε = 59,000) and is formed by two sequential one-electron oxidations of TMB. Thus the stoichiometry of oxidation is 0.5 mole charge transfer complex (λmax= 652 nm) or 1 mole of diimine (λmax= 450 nm) formed (or TMB oxidized) per mole of hydroperoxide reduced by the peroxidase.
3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate (TMB-d) is a sensitive chromogen substrate for peroxidase. It is non- carcinogenic, hence, it can be used as a safe substitute for benzidine. TMB-d is useful in quantifying hemoglobin and in staining myeloperoxidase-containing granules in granulocytes.

Basic Info

Product Name:

3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE

Other Name:

TMB2HC1 hydrate;TMB*2HCL*2H2O;3,3',5,5'-tetramethylbenzidine·2HCl·2H2O;3,3',5,5'-Tetramethyl-[1,1'-biphenyl]-4,4'-diamine hydrochloride hydrate(1:2:x);3,3',5,5'-tetramethylbiphenyl-4,4'-diamine hydrochloride hydrate;TMB-2HCL-2HO;5'-Tetramethylbenzidine 2HCl 2H20;3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE, 98+%

CAS No.:

207738-08-7

Molecular Formula:

C16H23ClN2O

InChIKeys:

NYNRGZULARUZCC-UHFFFAOYSA-N

Molecular Weight:

294.82

Exact Mass:

312.11600

EC Number:

688-511-0

TSCA:

Yes

DSSTox ID:

DTXSID10597911

Color/Form:

Light brown to brown

HScode:

29215900

Categories:

Biochemical Reagents

Characteristics

PSA:

52.04000

XLogP3:

6.51800

Melting Point:

>300°C

Boiling Point:

459.8ºC at 760 mmHg

Flash Point:

231.9ºC

Water Solubility:

soluble in DMSO (~1.7 mg/ml), and 1:300 DMSO:PBS (pH 7.2) (0.03 mg/ml).

Storage Conditions:

2-8ºC

Vapor Pressure:

3.01E-09mmHg at 25°C

Critical Temperature & Pressure:

2-8°C

See More 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE Properties 

Hazard Identification

Classification of the substance or mixture

Acute toxicity - Category 4, Oral

Acute toxicity - Category 4, Dermal

Acute toxicity - Category 4, Inhalation

Germ cell mutagenicity, Category 2

GHS label elements, including precautionary statements

Precautionary statement(s)
Pictogram(s)
Signal word	Warning
Hazard statement(s)	H302 Harmful if swallowed H312 Harmful in contact with skin H332 Harmful if inhaled H341 Suspected of causing genetic defects
Prevention	P264 Wash ... thoroughly after handling. P270 Do not eat, drink or smoke when using this product. P280 Wear protective gloves/protective clothing/eye protection/face protection/hearing protection/... P261 Avoid breathing dust/fume/gas/mist/vapours/spray. P271 Use only outdoors or in a well-ventilated area. P203 Obtain, read and follow all safety instructions before use.
Response	P301+P317 IF SWALLOWED: Get medical help. P330 Rinse mouth. P302+P352 IF ON SKIN: Wash with plenty of water/... P317 Get medical help. P321 Specific treatment (see ... on this label). P362+P364 Take off contaminated clothing and wash it before reuse. P304+P340 IF INHALED: Remove person to fresh air and keep comfortable for breathing. P318 IF exposed or concerned, get medical advice.
Storage	P405 Store locked up.
Disposal	P501 Dispose of contents/container to an appropriate treatment and disposal facility in accordance with applicable laws and regulations, and product characteristics at time of disposal.

Other hazards which do not result in classification

no data available

Handling and Storage

Precautions for safe handling

Handling in a well ventilated place. Wear suitable protective clothing. Avoid contact with skin and eyes. Avoid formation of dust and aerosols. Use non-sparking tools. Prevent fire caused by electrostatic discharge steam.

Conditions for safe storage, including any incompatibilities

Store the container tightly closed in a dry, cool and well-ventilated place. Store apart from foodstuff containers or incompatible materials.

See More 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE Safety Data Sheet 

Seller Information

Business Type:
Manufactory

Main Products:
Coenzymes,Inhibito,Enzymes,Zymogens,Substrates,Native Microorganism Creatine Amidinohydrolase

Location:
Shirley, New York 11967, USA

Payment Terms:
TT against copy of documents,D/P

Average lead Time:
15 days

Total Annual Revenue:
$1 million-$2.5 million

Year of Establishment:
2004

Creative Enzymes is a leading Manufactory supplier of Proline dipeptidase, Glucoamylase, BenzaMide, N-1H-pyrrolo[2,3-c]pyridin-5-yl- based in America. With a commitment to quality and customer satisfaction, Creative Enzymes has become a trusted name in the industry. Our product line includes a range of high-quality Proline dipeptidase, Glucoamylase, BenzaMide, N-1H-pyrrolo[2,3-c]pyridin-5-yl-, which are available at competitive prices. Creative Enzymes has established itself as a reliable supplier of chemical products on the echemi.com.
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3,3,5,5-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE, 98+%	/99.00		HANGZHOU LEAP CHEM CO., LTD.	Inquiry
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3,3',5,5'-Tetramethylbenzidine dihydrochloride hydrate

Product Description

Seller Information