 Home > Encyclopedia > 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE

3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE

3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE structure

CAS No:
207738-08-7
Formula:
C16H23ClN2O
Chemical Name:
3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE
Synonyms:
TMB2HC1 hydrate;TMB*2HCL*2H2O;3,3',5,5'-tetramethylbenzidine·2HCl·2H2O;3,3',5,5'-Tetramethyl-[1,1'-biphenyl]-4,4'-diamine hydrochloride hydrate(1:2:x);3,3',5,5'-tetramethylbiphenyl-4,4'-diamine hydrochloride hydrate;TMB-2HCL-2HO;5'-Tetramethylbenzidine 2HCl 2H20;3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE, 98+%
Categories:
Biochemical Engineering > Biochemical Reagents

Description

Off-white fluffy powder

TMB is an aromatic amine that undergoes oxidation by the higher oxidation states of heme peroxidases (compounds I and II) thereby serving as a reducing co-substrate. One electron oxidation of TMB results in a radical cation that forms a charge-transfer complex with the unoxidized compound. This charge transfer complex absorbs at 652 nm (ε = 39,000). The completely oxidized form (diimine) absorbs at 450 nm (ε = 59,000) and is formed by two sequential one-electron oxidations of TMB. Thus the stoichiometry of oxidation is 0.5 mole charge transfer complex (λmax= 652 nm) or 1 mole of diimine (λmax= 450 nm) formed (or TMB oxidized) per mole of hydroperoxide reduced by the peroxidase.

3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate (TMB-d) is a sensitive chromogen substrate for peroxidase. It is non- carcinogenic, hence, it can be used as a safe substitute for benzidine. TMB-d is useful in quantifying hemoglobin and in staining myeloperoxidase-containing granules in granulocytes.

3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE Basic Attributes

Molecular Weight：

294.82

Exact Mass：

312.11600

EC Number：

688-511-0

TSCA：

Yes

DSSTox ID：

DTXSID10597911

Color/Form：

Light brown to brown

HScode：

29215900

Characteristics

PSA：

52.04000

XLogP3：

6.51800

Melting Point：

>300°C

Boiling Point：

459.8ºC at 760 mmHg

Flash Point：

231.9ºC

Water Solubility：

soluble in DMSO (~1.7 mg/ml), and 1:300 DMSO:PBS (pH 7.2) (0.03 mg/ml).

Storage Conditions：

2-8ºC

Vapor Pressure：

3.01E-09mmHg at 25°C

Critical Temperature & Pressure：

2-8°C

Safety Information

Transport codes：

NONH for all modes of transport

wgk germany：

hazard class code：

safety instructions：

22-24/25-25-24

Hazard Statements：

GHS Classification：

|Warning|H302 (100%): Harmful if swallowed [Warning Acute toxicity, oral]|P201, P202, P261, P264, P270, P271, P280, P281, P301+P312, P302+P352, P304+P312, P304+P340, P308+P313, P312, P322, P330, P363, P405, and P501|The GHS information provided by 1 company from 1 notification to the ECHA C&L Inventory.

3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE Use and Manufacturing

3,3',5,5'-Tetramethylbenzidine dihydrochloride hydrate is a chromophore substrate in ELISA assays. It is a noncarcinogenic substitute for benzidine.

Convenient water-soluble form of TMB for determination of peroxidase.

3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate has also been used as a horseradish peroxidase substrate in enzyme linked immunosorbent assay and reverse dot blot (RDB).

Computed Properties

Molecular Weight:349.3
Hydrogen Bond Donor Count:6
Hydrogen Bond Acceptor Count:4
Rotatable Bond Count:1
Exact Mass:348.1371335
Monoisotopic Mass:348.1371335
Topological Polar Surface Area:54
Heavy Atom Count:22
Complexity:226
Covalently-Bonded Unit Count:5
Compound Is Canonicalized:Yes

Recommended Suppliers of 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE

HANGZHOU LEAP CHEM CO., LTD. Contact Supplier

CN
5 YRS

Business licensed

Trader Supplier of Intermediates,Building blocks,API,Silicones,Peptides,Lab chemicals,Biochemicals,Pharmaceuticals,Screening Compounds,Food Additives

3,3,5,5-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE, 98+%

CAS No.: 207738-08-7

Content: 99.00%

Inquiry
Creative Enzymes Contact Supplier

US
6 YRS

Business licensed Certified factory

Manufactory Supplier of Coenzymes,Inhibito,Enzymes,Zymogens,Substrates

3,3',5,5'-Tetramethylbenzidine dihydrochloride hydrate

CAS No.: 207738-08-7

Inquiry
Henan Lihao Chem Plant Limited Contact Supplier

CN
5 YRS

Business licensed

Trader Supplier of PVC resin,pvc paste resin,melamine

TMB dihydrochloride 99% white powder kanbei

CAS No.: 207738-08-7

Grade: Industrial Grade

Content: 99%

Inquiry

Learn More Other Chemicals

Benzenecarboximidamide, 4,4′-iminobis-, dihydrochloride

21055-30-1
2,3-diaMino-5-broMobenzoic acid dihydrochloride

58580-09-9
1,5-Pentanediaminium, N1,N1,N5,N5-tetramethyl-N1,N5-bis[1-methyl-3-(2,6,6-trimethyl-1-cyclohexen-1-yl)propyl]-, bromide, hydrate (2:4:3)

66827-13-2
Glycine, N-2-naphthalenyl-, hydrazide, dihydrochloride Formula

70700-31-1
L-Cystine, radical ion(1+), dihydrochloride Formula

13059-63-7
1-(3,4-DICHLORO-BENZYL)-PYRROLIDIN-3-YLAMINE DIHYDROCHLORIDE Formula

828928-33-2
L-Iditol, 1-deoxy-1-nitro-, hydrate (2:1) Structure

207226-23-1
Dibromopropamidine Dihydrochloride Structure

50357-61-4
What is 1-(2,4-DICHLOROPHENYL)-PIPERAZINE DIHYDROCHLORIDE

827614-48-2
What is 2-CYCLOHEXYL-1 H-BENZOIMIDAZOL-5-YLAMINE DIHYDROCHLORIDE

1158326-68-1

Hot Searches: formula for potassium sulfide; aluminum hydroxide formula; oxytetracycline; isopentane; pbso4; 2-methyl-2-butene

Guide of 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE

Description
Basic Attributes
Characteristics
Safety Information
Use and Manufacturing
Computed Properties
Recommended Suppliers
Learn More Other Chemicals

3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE

SDS

Request for Quotation



Feedback & Suggestions

Send Message

Thank you for your feedback. If you require further assistance, please contact us by email at info@echemi.com or call us at +86-532-55729510.