Native Bacillus stearothermophilus Polynucleotide Phosphorylase
9014-12-4
2026-06-26
Coenzymes,Inhibito,Enzymes,Zymogens,Substrates,Native Microorganism Creatine Amidinohydrolase
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Product Description
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Seller Information
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Description
ProductName Native Bacillus stearothermophilus Polynucleotide Phosphorylase Cat No NATE-1908 Description Polynucleotide phosphorylase (PNPase) is a bifunctional enzyme with a phosphorolytic 3' to 5' exoribonuclease activity and a 3'-terminal oligonucleotide polymerase activity. It is also involved in mRNA processing and degradation in bacteria, plants, and humans. Abbr PNPase, Native (Bacillus stearothermophilus) Alias PNPase Source Bacillus stearothermophilus Applications The enzyme is useful for the preparation of polyribonucleotide. Appearance Lyophilized Enzyme Commission Number EC 2.7.7.8 CAS No 9014-12-4 Molecular Weight 300,000 - 340,000; Subunit molecular weight : ca. 85,000. Isoelectric point 4 pH Stability 9.0 - 11.0 Michaelis Constant (38 mM Tris-HCI buffer, pH 9.5, at 60 °C)Poly A: 0.27 mM**;KH2PO4: 3.0 mM;**concentration of poly A was calculated as AMP concentration. Unit Definition One unit of activity is defined as the amount of PNPase that forms 1 μmol of ADP per hour at 60 °C by depolymerizing of Poly A. Optimum pH 9.0 - 9.5 Thermal stability No detectable decrease in activity up to 55 °C. Storage Stable at -20 °C for at least one year. Synonyms PNPase; nucleoside diphosphate:polynucleotidyl transferase; polyribonucleotide nucleotidyltransferase; polynucleotide phosphorylase; polyribonucleotide phosphorylase; EC 2.7.7.8; 9014-12-4 Specific Activity more than 2,000 U/mg protein Reaction RNAn+1 + Pi ←→ RNAn + Nucleoside diphosphate Notes Effectors: cations and anions. category Specialty Chemicals(c26) cas_num 9014-12-4 Basic Info-
Product Name:
Polyribonucleotide nucleotidyltransferase
Other Name:Polyribonucleotide nucleotidyltransferase;Nucleotidyltransferase,polyribonucleotide;Polynucleotide phosphorylase;E.C. 2.7.7.8;Polyribonucleotide phosphorylase;Nuclear phosphorolytic nuclease;PNPase;Nucleotide phosphorylase
CAS No.:9014-12-4
Categories:
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Seller Information
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Business Type:
Manufactory
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Main Products:
Coenzymes,Inhibito,Enzymes,Zymogens,Substrates,Native Microorganism Creatine Amidinohydrolase
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Location:
Shirley, New York 11967, USA
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Payment Terms:
TT against copy of documents,D/P
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Average lead Time:
15
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Total Annual Revenue:
$1 million-$2.5 million
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Year of Establishment:
2004
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