1. Description
Chemical properties: FOXO4-DRI belongs to linear peptide compounds, with an amino acid sequence of Ac Gly Pro Trp Gly Leu Met Gly Gln Gly Leu Phe Gly Gly Gly COOH. The N-terminus is acetylated to enhance enzymatic resistance and lipophilicity. The core target is FOXO4 protein (forkhead box protein O4) in senescent cells, which exerts activity by blocking the interaction between FOXO4 and p53. The chemical properties are stable, and it is not easily hydrolyzed in neutral to weakly alkaline (pH 6.0-8.0) environments. Its anti peptidase degradation ability is superior to short chain peptides, and it has good compatibility with active ingredients such as coenzyme NAD+, glutathione, collagen, etc. Its molecular weight is 1316.5 Da
Physical properties: FOXO4-DRI is a white to off white crystalline powder at room temperature, odorless and tasteless, with excellent water solubility (solubility ≥ 40 mg/mL). It can quickly dissolve in pure water, physiological saline, phosphate buffer solution (PBS), and water-based matrices such as glycerol and propylene glycol. It is slightly soluble in ethanol and insoluble in non-polar solvents such as ether and petroleum ether. Store under dry, sealed, and dark conditions at -20 ℃ to avoid repeated freezing and thawing; The purity of research grade/application grade products is ≥ 98% (HPLC detection), with single impurities ≤ 1.0%, no endotoxins (≤ 0.05 EU/mg), and no residual heavy metals (lead, mercury, arsenic, etc. ≤ 0.1 ppm), meeting the standards for medicinal raw materials and high-end health raw materials.
Source and preparation: FOXO4-DRI has no natural form and is accurately prepared by solid-phase peptide synthesis method - using high-purity L-type amino acids as raw materials, condensation, deprotection, acetylation modification, peptide chain assembly, and finally purified by high performance liquid chromatography (HPLC) to obtain high-purity products; The synthesis process strictly controls the reaction conditions (temperature, coupling efficiency) to ensure the integrity of the peptide chain structure and biological activity. The product quality is verified through mass spectrometry (MS), amino acid sequence analysis, and targeted binding activity detection, with no residual chemical synthesis by-products.
2. Application
1. Anti aging health intervention field (core scenario)
High end anti-aging oral/injectable products: FOXO4-DRI is suitable for 35+people with functional decline, middle-aged and elderly people, and people with premature aging constitution. It is used to develop anti-aging dietary supplements, injectable anti-aging preparations, sublingual drops, etc; The core function is to eliminate accumulated aging cells in the body (such as skin fibroblasts, liver cells, and muscle cells), delay tissue aging, and improve aging related symptoms (skin laxity, decreased energy, and slowed metabolism); Compound logic: FOXO4-DRI (added concentration 0.1% -0.5%)+NAD+(5% -10%)+glutathione (2% -5%), FOXO4-DRI clears aging, NAD+repairs cellular energy metabolism, glutathione antioxidant, synergistically achieves the triple anti-aging of "clearing aging+repairing+antioxidant".
Local anti-aging repair products: FOXO4-DRI is used to develop high-end anti-aging face cream and scalp anti-aging essence for skin aging (relaxation, wrinkles, dullness) and hair aging (hair loss, hair quality deterioration); Compound logic: FOXO4-DRI (0.05% -0.1%)+recombinant collagen (0.05% -0.1%)+ceramide (0.2% -0.3%), clears aging fibroblasts in the dermis of the skin, promotes proliferation of new cells, and improves skin firmness and elasticity.
2. Biomedical research field
Aging mechanism research tool reagent: FOXO4-DRI is used to explore the mechanisms of cellular aging models (such as replicative aging cells and oxidative stress-induced aging cells) and animal aging models (such as natural aging mice and premature aging model mice), analyze the effects of aging cell clearance on tissue repair, lifespan extension, and disease prevention, and provide experimental evidence for the development of anti-aging drugs.
Research materials for age-related diseases: FOXO4-DRI is used for model studies of age-related diseases such as Alzheimer's disease, osteoporosis, metabolic syndrome, and cardiovascular disease, exploring the possibility of intervening in disease progression by clearing senescent cells. It is a core tool peptide for Senolytics drug development.
3. High end beauty extension applications
Luxury anti-aging skin care products: FOXO4-DRI is aimed at high-end anti-aging consumer groups, and is used to develop anti-aging face cream, firming essence, and anti wrinkle lotion; Adding a concentration of 0.03% -0.1%, a compound of bosein (3% -5%) and retinol derivatives (0.1% -0.3%), FOXO4-DRI removes aging skin cells, bosein strengthens dermal support, and retinol promotes cell regeneration, synergistically improving skin laxity, static lines, and dull skin tone, achieving "deep layer anti-aging+surface wrinkle resistance".
Anti aging and efficiency enhancing products after medical art: FOXO4-DRI is used to develop repair and anti-aging essence after being adapted to anti-aging medical beauty projects such as ReMagee, ultrasonic scalpel, etc. FOXO4-DRI can remove the damaged aging cells after medical beauty, accelerate the proliferation of new cells, improve the durability of anti-aging effect of medical beauty, and compound Ekedorine (1% -2%)+Panthenol (2% -3%), giving consideration to repair and anti-aging.
3. Main Efficacy
Targeted clearance of senescent cells (core efficacy): The core mechanism of FOXO4-DRI is "precise recognition+selective apoptosis" - FOXO4-DRI specifically binds to FOXO4 protein in senescent cells, blocking its interaction with p53 protein (in normal cells, FOXO4 binds to p53 to maintain cell stability, while in senescent cells, this binding leads to sustained cell survival and the release of senescence related secretory phenotype SASP); After unbinding, the p53 protein initiates the apoptosis program of senescent cells without causing damage to normal cells (FOXO4-p53 binding is not necessary for survival in normal cells, and FOXO4-DRI has low binding affinity for normal cells), achieving "clear aging without damaging positivity".
Delayed tissue aging and functional enhancement effects: FOXO4-DRI clears accumulated aging cells in the body, reduces SASP (inflammatory factors, matrix metalloproteinases, etc.) damage to surrounding normal cells, alleviates chronic inflammation, and promotes tissue repair and regeneration; In animal models, it can improve skin elasticity, enhance muscle strength, improve cognitive function, delay organ function decline, and prolong the healthy lifespan of model animals (Healthspan).
Skin anti-aging and repairing effects: FOXO4-DRI removes aging fibroblasts from the dermis layer of the skin, activates the proliferation and activity of newly generated fibroblasts, promotes the synthesis of type I and III collagen and elastic fibers, and improves skin laxity, static lines, and enlarged pores; At the same time, it reduces skin inflammation and pigmentation caused by SASP, brightens skin tone, enhances skin barrier function, and has significantly better anti-aging effects on mature skin than traditional anti wrinkle ingredients.
Safety advantages: FOXO4-DRI, as an artificially synthesized peptide, has extremely high biocompatibility, no immunogenicity, and does not cause allergic or rejection reactions; The cytotoxicity test (MTT) showed that when the concentration was ≤ 1 μ mol/L, the normal cell survival rate was>98%, and only had an apoptosis inducing effect on senescent cells; Long term use has no significant side effects, no dependence, and does not affect the physiological functions of normal cells. It is suitable for long-term anti-aging interventions and health maintenance scenarios.
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