1. Product Overview
MI-503 (CAS 1857417-13-0), also known as 1-((1H-Pyrazol-4-yl)methyl)-4-methyl-5-((4-((6-(2,2,2-trifluoroethyl)thieno[2,3-d]pyrimidin-4-yl)amino)piperidin-1-yl)methyl)-1H-indole-2-carbonitrile, is a potent and selective small-molecule inhibitor targeting the Menin–MLL interaction. With a molecular formula of C₂₈H₂₇F₃N₈S and a molecular weight of 564.63, it appears as a white to off-white crystalline solid at room temperature. MI-503 is widely used in epigenetic research and oncology studies focused on MLL-rearranged leukemias and other cancers driven by Menin-MLL dependency. This product is supplied for laboratory research use only and is not intended for clinical, diagnostic, or therapeutic applications in humans.
2. Key Features and Advantages
• High potency: Selective Menin–MLL inhibitor with an IC₅₀ of approximately 14.7 nM.
• High purity: Typically ≥98% by HPLC, suitable for sensitive cell-based and biochemical assays.
• Well-characterized epigenetics tool compound for studying MLL-fusion leukemia pathways.
• Good solubility in DMSO (up to ~50 mg/mL), enabling convenient stock preparation for in vitro studies.
• Consistent batch quality with supporting analytical data such as HPLC and NMR upon request.
3. Main Applications
• Epigenetics and cancer research, particularly investigating Menin–MLL interaction inhibition in MLL-rearranged acute leukemias.
• In vitro biochemical assays to evaluate Menin binding disruption and downstream target gene regulation (e.g., HOXA9, MEIS1).
• Cell-based proliferation and apoptosis studies using MLL-dependent leukemia cell lines (such as MV4-11).
• Mechanistic studies on epigenetic dysregulation and targeted therapy development in hematologic malignancies.
• General research use as a reference standard or probe compound in medicinal chemistry and drug discovery programs focusing on menin inhibitors.
4. Technical Specifications
5. Storage and Handling
• Store the powder sealed in a cool, dry place protected from light; recommended storage at -20°C. For long-term storage of DMSO stock solutions, aliquot and store at -80°C to avoid repeated freeze–thaw cycles.
• Keep container tightly closed and desiccated; avoid exposure to moisture, heat, and direct sunlight.
• Before opening, allow the sealed package to warm to room temperature to prevent condensation.
• Handle in a well-ventilated area or fume hood. Use personal protective equipment including safety glasses, protective gloves, and a lab coat. Avoid inhalation of dust and contact with skin or eyes.
• Intended exclusively for laboratory research by qualified personnel; not for human or veterinary use, food, drug, or cosmetic applications.
6. Safety Overview
• MI-503 is a research chemical with limited toxicological data. Treat as potentially hazardous: may cause irritation to eyes, skin, and respiratory system.
• In case of contact with eyes, rinse immediately with plenty of water for at least 15 minutes and seek medical advice. In case of skin contact, wash thoroughly with soap and water.
• If inhaled, move the person to fresh air and keep at rest in a position comfortable for breathing. If ingested, rinse mouth and seek medical attention—do not induce vomiting unless directed by medical personnel.
• Use appropriate engineering controls (fume hood) and personal protective equipment (safety goggles, nitrile gloves, lab coat). Avoid generating dust.
• Dispose of in accordance with local regulations for chemical waste. Do not discharge into drains, waterways, or soil.
• For detailed safety information, refer to the applicable Safety Data Sheet (SDS) prior to handling.
