Cyanidin-3-O-sambubioside-5-O-glucoside chloride 98% Purity Anthocyanin Reference Standard for Natural Product Analysis and Anthocyanin Research CAS 5

Product Overview

Cyanidin-3-O-sambubioside-5-O-glucoside chloride (CAS 53925-33-0) is a high‑purity anthocyanin reference standard featuring cyanidin as the aglycone with a sambubioside moiety (glucose‑xylose disaccharide) at the 3‑position and a glucose moiety at the 5‑position. The molecular formula is C₃₂H₃₇ClO₂₀ with a molecular weight of 777.08 g/mol. Sambubioside is a disaccharide composed of glucose and xylose linked via a β‑1→2 glycosidic bond. This complex glycosylation pattern is characteristic of anthocyanins found in various plant species, particularly in elderberries (Sambucus nigra) and other members of the Caprifoliaceae family. As a reference standard, it is essential for the identification and quantification of complex anthocyanin glycosides in plant extracts, food products, and nutraceuticals using HPLC‑DAD and UPLC‑MS/MS methods.

Synthetic Process / Isolation Method

This compound is typically isolated from natural plant sources through a series of extraction and chromatographic purification steps:

Extraction: Plant materials rich in anthocyanins (such as elderberries) are extracted with acidified methanol or ethanol.

Partitioning: The crude extract is subjected to liquid‑liquid partitioning to concentrate the anthocyanin fraction.

Column Chromatography: The enriched fraction undergoes repeated column chromatography using Amberlite XAD‑7, Sephadex LH‑20, or preparative HPLC with acidic mobile phases.

Recrystallization: The purified compound is recrystallized to achieve ≥98% purity.

Quality Confirmation: The final product is rigorously characterized by HPLC‑PDA, ¹H‑NMR, ¹³C‑NMR, and high‑resolution mass spectrometry.

Quality Specifications

Our Cyanidin-3-O-sambubioside-5-O-glucoside chloride is manufactured under strict quality control conditions: - Purity: ≥98.0% determined by HPLC - Identity confirmed by NMR and mass spectrometry - Water content: ≤1.0% - Heavy metals: ≤20 ppm - Each batch accompanied by comprehensive Certificate of Analysis (COA) - Stable for 24 months when stored at -20°C in sealed containers protected from light and moisture

Packaging & Shipping

We offer flexible packaging options to meet diverse customer needs: - Research quantities: 2mg, 5mg, 10mg amber glass vials with PTFE‑lined caps, purged with inert gas - Bulk quantities: Available upon request - Custom packaging available upon request - Shipped with blue ice for temperature‑sensitive orders - International shipping available with proper documentation

Jinan Topfull Chemical Co., Ltd. is an international company rooted in China, specializing in the export of chemical products.

Our company is committed to making every effort to provide ideal chemical products to potential customers worldwide, including but not limited to: organic/inorganic reagents, biological reagents, plant extracts, pharmaceutical intermediates, active pharmaceutical ingredients (APIs), reference standards, reference substances, peptides, and more. Meanwhile, we have established long-term and stable cooperative relationships with university laboratories and off-campus research institutions, enabling us to offer tailored product services based on customers' actual needs.

We currently support customized services for most product categories, with supply quantities ranging from milligram (mg), gram (g) to kilogram (kg) levels, as well as wholesale. Product purity covers the full spectrum from 90% to 99.9%, providing customers with one-stop convenient services.

To date, our products have been exported to developed countries such as the United States, Australia, Canada, Mexico, as well as EU member states including France, Germany, and many other countries and regions such as Nigeria, Ghana, Southeast Asia, and other parts of Asia. Our product quality and services have consistently received high praise.

Looking ahead, our company will continue to serve as a bridge connecting China and the world, delivering high-quality products and services to global customers.