Product Overview
Paris saponin V palmitate (CAS 1459260-68-4) is a high‑purity steroidal saponin reference standard featuring progenin III (a diosgenin glycoside) as the aglycone with a palmitic acid ester moiety at the 3‑position of the terminal rhamnose. The molecular formula is C₅₁H₈₄O₁₃ with a molecular weight of 881.23 g/mol. This compound is a fatty acid esterified derivative of a major bioactive saponin isolated from the rhizomes of Paris polyphylla (Chong Lou in Chinese), a well‑known medicinal plant in traditional Chinese medicine used for the treatment of inflammation, cancer, and various infectious diseases. The addition of palmitic acid enhances the lipophilicity of the compound, potentially improving its bioavailability and pharmacokinetic properties. As a research‑grade reference standard, it is essential for the identification and quantification of esterified saponin derivatives in plant extracts and herbal products using HPLC‑DAD and UPLC‑MS/MS methods.
Synthetic Process / Isolation Method
This compound is typically isolated from the rhizomes of Paris polyphylla through a series of extraction and chromatographic purification steps:
Extraction: Dried rhizomes of Paris polyphylla are extracted with aqueous ethanol or methanol under reflux.
Partitioning: The crude extract is subjected to liquid‑liquid partitioning with n‑butanol to concentrate the saponin fraction.
Column Chromatography: The enriched fraction undergoes repeated column chromatography using silica gel, macroporous resin, or Sephadex LH‑20, eluting with gradient solvent systems such as chloroform‑methanol‑water.
Preparative HPLC: Final purification is achieved using preparative HPLC with a C18 reversed‑phase column.
Recrystallization: The purified compound is recrystallized to achieve ≥98% purity.
Quality Confirmation: The final product is rigorously characterized by HPLC‑PDA, ¹H‑NMR, ¹³C‑NMR, 2D‑NMR (COSY, HMBC, HSQC), and high‑resolution mass spectrometry.
Mechanism of Action
As a steroidal saponin with a palmitate ester moiety, paris saponin V palmitate exerts its biological activities through multiple mechanisms:
Antitumor Activity: Induces apoptosis in cancer cells through the activation of caspase‑dependent pathways and modulation of mitochondrial membrane potential. Studies have shown that paris saponin derivatives exhibit cytotoxic effects against various human cancer cell lines including HepG2, MCF‑7, and A549.
Anti‑inflammatory Activity: Modulates inflammatory signaling pathways including NF‑κB, reducing the production of pro‑inflammatory cytokines such as TNF‑α, IL‑1β, and IL‑6.
Enhanced Bioavailability: The fatty acid ester moiety increases lipophilicity, potentially enhancing cell membrane permeability and oral bioavailability compared to non‑esterified saponins.
Antimicrobial Activity: Exhibits inhibitory effects against certain bacterial and fungal strains.
Quality Specifications
Our Paris saponin V palmitate is manufactured under strict quality control conditions: - Purity: ≥98.0% determined by HPLC - Identity confirmed by NMR and mass spectrometry - Water content: ≤1.0% - Heavy metals: ≤20 ppm - Each batch accompanied by comprehensive Certificate of Analysis (COA) - Stable for 24 months when stored at 2‑8°C in sealed containers protected from light and moisture
Packaging & Shipping
We offer flexible packaging options to meet diverse customer needs: - Research quantities: 2mg, 5mg, 10mg amber glass vials with PTFE‑lined caps, purged with inert gas - Bulk quantities: Available upon request - Custom packaging available upon request - Shipped at ambient temperature for routine orders - Cold chain shipping available upon request for temperature‑sensitive orders - International shipping available with proper documentation
Jinan Topfull Chemical Co., Ltd. is an international company rooted in China, specializing in the export of chemical products.
Our company is committed to making every effort to provide ideal chemical products to potential customers worldwide, including but not limited to: organic/inorganic reagents, biological reagents, plant extracts, pharmaceutical intermediates, active pharmaceutical ingredients (APIs), reference standards, reference substances, peptides, and more. Meanwhile, we have established long-term and stable cooperative relationships with university laboratories and off-campus research institutions, enabling us to offer tailored product services based on customers' actual needs.
We currently support customized services for most product categories, with supply quantities ranging from milligram (mg), gram (g) to kilogram (kg) levels, as well as wholesale. Product purity covers the full spectrum from 90% to 99.9%, providing customers with one-stop convenient services.
To date, our products have been exported to developed countries such as the United States, Australia, Canada, Mexico, as well as EU member states including France, Germany, and many other countries and regions such as Nigeria, Ghana, Southeast Asia, and other parts of Asia. Our product quality and services have consistently received high praise.
Looking ahead, our company will continue to serve as a bridge connecting China and the world, delivering high-quality products and services to global customers.
