CAS 848784-87-2 Research Grade Compound

• Product Overview

  Lup-20(29)-en-28-oic acid-3-O-[α-L-rhamnopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→4)]-α-L-arabinopyranoside] (CAS 848784-87-2) is a high‑purity triterpenoid saponin reference standard featuring a lupane‑type triterpene aglycone with a branched trisaccharide chain attached at the 3‑position. The sugar chain consists of three monosaccharide units: rhamnose-(1→2)-[glucose-(1→4)]-arabinose. The compound is a naturally occurring saponin found in various plant species, including members of the families Fabaceae, Rosaceae, and Lamiaceae. Lupane‑type triterpenoids are known for their diverse biological activities including anti‑inflammatory, antitumor, antimicrobial, and immunomodulatory effects. As a research‑grade reference standard, it is essential for the identification and quantification of triterpenoid saponins in plant extracts and herbal products using HPLC‑DAD and UPLC‑MS/MS methods.

  Synthetic Process / Isolation Method

  This compound is typically isolated from natural plant sources through a series of extraction and chromatographic purification steps:

  • Extraction: Dried plant materials (such as leaves, bark, or roots) are extracted with aqueous ethanol or methanol under reflux.

  • Partitioning: The crude extract is subjected to liquid‑liquid partitioning with n‑butanol to concentrate the saponin fraction.

  • Column Chromatography: The enriched fraction undergoes repeated column chromatography using silica gel, macroporous resin, or Sephadex LH‑20, eluting with gradient solvent systems such as chloroform‑methanol‑water.

  • Preparative HPLC: Final purification is achieved using preparative HPLC with a C18 reversed‑phase column.

  • Recrystallization: The purified compound is recrystallized to achieve ≥98% purity.

  • Quality Confirmation: The final product is rigorously characterized by HPLC‑PDA, ¹H‑NMR, ¹³C‑NMR, 2D‑NMR (COSY, HMBC, HSQC), and high‑resolution mass spectrometry.

  Mechanism of Action

  As a lupane‑type triterpenoid saponin, this compound exerts its biological activities through multiple mechanisms:

  • Anti‑inflammatory Activity: Modulates inflammatory signaling pathways including NF‑κB and MAPK, reducing the production of pro‑inflammatory cytokines such as TNF‑α, IL‑1β, and IL‑6.

  • Antitumor Activity: Induces apoptosis in cancer cells through the activation of caspase‑dependent pathways and modulation of mitochondrial membrane potential.

  • Antimicrobial Activity: Exhibits inhibitory effects against certain bacterial and fungal strains.

  • Immunomodulatory Effects: Enhances immune function by activating macrophages and promoting the production of immunomodulatory cytokines.

  Quality Specifications

  Our Lup-20(29)-en-28-oic acid-3-O-[α-L-rhamnopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→4)]-α-L-arabinopyranoside] is manufactured under strict quality control conditions: - Purity: ≥98.0% determined by HPLC - Identity confirmed by NMR and mass spectrometry - Water content: ≤1.0% - Heavy metals: ≤20 ppm - Each batch accompanied by comprehensive Certificate of Analysis (COA) - Stable for 24 months when stored at 2‑8°C in sealed containers protected from light and moisture

  Packaging & Shipping

  We offer flexible packaging options to meet diverse customer needs: - Research quantities: 5mg, 10mg amber glass vials with PTFE‑lined caps, purged with inert gas - Bulk quantities: Available upon request - Custom packaging available upon request - Shipped at ambient temperature for routine orders - Cold chain shipping available upon request for temperature‑sensitive orders - International shipping available with proper documentation

Jinan Topfull Chemical Co., Ltd. is an international company rooted in China, specializing in the export of chemical products.

Our company is committed to making every effort to provide ideal chemical products to potential customers worldwide, including but not limited to: organic/inorganic reagents, biological reagents, plant extracts, pharmaceutical intermediates, active pharmaceutical ingredients (APIs), reference standards, reference substances, peptides, and more. Meanwhile, we have established long-term and stable cooperative relationships with university laboratories and off-campus research institutions, enabling us to offer tailored product services based on customers' actual needs.

We currently support customized services for most product categories, with supply quantities ranging from milligram (mg), gram (g) to kilogram (kg) levels, as well as wholesale. Product purity covers the full spectrum from 90% to 99.9%, providing customers with one-stop convenient services.

To date, our products have been exported to developed countries such as the United States, Australia, Canada, Mexico, as well as EU member states including France, Germany, and many other countries and regions such as Nigeria, Ghana, Southeast Asia, and other parts of Asia. Our product quality and services have consistently received high praise.

Looking ahead, our company will continue to serve as a bridge connecting China and the world, delivering high-quality products and services to global customers.