Glycozolinine

Giving mice 20.0ml and 10.0ml/kg body weight of 28-flavor kidney-tonifying oral liquid (equivalent to 11.2g and 5.6g/kg of crude drug, respectively) can significantly improve the sexual function of male mice and increase the number of vaginal plugs in female mice in the same cage. 28-flavor kidney-tonifying capsule (5.4g/kg of crude drug) also has a similar effect. High-dose oral liquid can increase the activity of SOD in the whole blood of mice, indicating that this product has a certain effect on delaying aging. 28-flavor kidney-tonifying capsule also has the same effect, but the effect of oral liquid (raw drug) 11.2g/kg of body weight is better than capsule (5.4g/kg of body weight). Acute toxicity test: 1g of 28-flavor kidney-tonifying oral liquid was given to mice, and the maximum daily tolerance was greater than 90ml/kg (equivalent to 50.4g of crude drug), which is 225 times the daily dose to be used clinically; ip administration, the maximum daily tolerance was greater than 40ml/kg (equivalent to 22.4g of crude drug), indicating that this product has little toxicity. Long-term toxicity test: rats were gavaged with twice-concentrated 28-flavor kidney-tonifying oral liquid, with a maximum daily dose of 15 ml/kg, equivalent to 16.8 g/kg body weight of crude drug, which is 75 times the proposed clinical daily dose. The drug was administered for 12 consecutive weeks. No toxic or adverse reactions were observed in the animal's appearance, body weight, organ coefficient, blood picture, blood biochemistry, and pathological examination of 12 major organ tissues, and no deaths occurred. The proposed clinical dose of this product is safe.