 Home > Encyclopedia > Thrombin

Thrombin

Thrombin structure

CAS No:
9002-04-4
Chemical Name:
Thrombin
Synonyms:
Thrombin;Thrombase;E.C. 3.4.4.13;E.C. 3.4.21.5;Thrombofort;Thrombin-C;Tropostasin;Blood-coagulation factor II,activated;Blood-coagulation factor IIa;Factor IIa;Thrombostat;Thrombin JMI;Thrombinar;Thrombin II;Evithrom;Thrombin/Factor IIa;Factor 2a;BP 25432;105881-84-3;8050-02-0;8059-56-1;9014-41-9;53028-63-0
Categories:
Biochemical Engineering > Enzymes and Coenzymes Drugs

Request for Quotation

Description

Thrombin is a trypsin-like allosteric serine protease that has a fundamental role in the clotting cascade.

Thrombin Basic Attributes

Molecular Weight：

263.75

Exact Mass：

263.028396

EC Number：

232-648-7

HScode：

30021099

Characteristics

PSA：

87.5

Appearance：

white powder

Melting Point：

21.3 °C

Water Solubility：

H2O: 1 mL/vial clear to slightly hazy, colorless solution

Storage Conditions：

−20°C

Safety Information

Transport codes：

NONH for all modes of transport

wgk germany：

hazard class code：

36/37/38-42

safety instructions：

22-24/25-36/37-26-24

RTECS Number：

XO8950000

Marks：

Xn,B,Xi

Precautionary statement(s)：

P261-P305 + P351 + P338-P342 + P311

Hazard Statements：

H315-H319-H334-H335

Thrombin Use and Manufacturing

Methods of Manufacturing

Raw material processing and precipitation Take 2kg of pig blood, add 200ml of sodium citrate (38g/L or 3.8%) solution for anticoagulation, mix well, separate out, take out the supernatant plasma, add 10 times the amount of distilled water to the plasma Dilute, adjust Ph to 5.3 with 300ml of acetic acid (1%), after the precipitation is complete, centrifuge in a centrifuge for 5-10 minutes, discard the centrifugal fluid, collect the precipitate to obtain prothrombin. Animal blood sodium citrate → raw material treated plasma distilled water: HAc; Ph5.3 → precipitation prothrombin separation, precipitation, washing to take prothrombin, at 25-30C0, add 700ml of sodium chloride (0.9%) solution, Dissolve it, then add 15g calcium chloride to each liter of solution, stir well for 10min to dissolve it, leave it in the cold room for 1h, centrifuge, take the supernatant and add the same amount of frozen acetone, stir well and let it stand overnight. After centrifugation, the precipitate is finely ground with frozen acetone, placed in a cold room for 2 to 3 days, filtered to obtain the precipitate, washed with ether, and dried in vacuum to obtain crude thrombin, about 10 g. Prothrombin NaCl; CaCl2; acetone→separation; precipitation precipitation ether→washing crude thrombin to remove impurities, precipitation and drying. Add 200ml sodium chloride solution (9g/L) to the crude thrombin to dissolve it. Place it at 00C for 6 hours, filter, take the precipitate and add 150ml sodium chloride solution (9g/L) to repeat the above operation once, and combine the two filtrates. Adjust Ph to 5.5 with acetic acid (1%). Centrifugal separation, precipitation discarded, take the supernatant and add 2 times the amount of frozen acetone to the supernatant, let it stand for 2 hours, after the precipitation is complete, centrifuge, the precipitate is finely ground with frozen acetone, placed for 24 hours, and the solution is filtered. The precipitate was washed with absolute ethanol and ether, and dried in vacuum to obtain refined thrombin, about 0.4 g. Crude thrombin NaCl; HAc; ^0<0C; pH5.5 → filtrate acetone → precipitation and dried refined products

Uses

Biochemical research

Drug Function and Efficacy

It promotes the conversion of fibrinogen into fibrin, which is applied to wounds to coagulate blood and stop bleeding.

This ingredient has been used in drugs with the following functions (note: it does not mean that the ingredient itself has the following health functions)

Related Drugs

Thrombin lyophilized powder
Ingredients	This product is a sterile lyophilized product of prothrombin extracted from bovine or pig blood and activated to obtain thrombin. The potency of each 1mg should not be less than 10 units. The thrombin content should be more than 80% of the labeled amount.
Indication	1. This product will react with acid, alkali and heavy metals and reduce its efficacy; 2. To improve the hemostatic effect of upper gastrointestinal bleeding, it is advisable to take a certain amount of antacid to neutralize gastric acid before taking this product orally, or give acid inhibitors intravenously at the same time; 3. This product can also be dissolved in phosphate buffer (pH 7.6) or cold milk. If a latex solution is prepared with gum arabic, gelatin, fructose gum, honey, etc., the hemostatic effect of thrombin can be improved, and the dosage of this product can be appropriately reduced.
Specifications	1000 units
Manufacturer	Wuhan Hiteck Biological Pharma Co., Ltd.

Thrombin
Ingredients	This product is a sterile lyophilized product of prothrombin extracted from bovine or pig blood and activated to obtain thrombin. The potency of each 1mg should not be less than 10 units. The thrombin content should be more than 80% of the labeled amount.
Indication	1. This product will react with acid, alkali and heavy metals and reduce its efficacy; 2. To improve the hemostatic effect of upper gastrointestinal bleeding, it is advisable to take a certain amount of antacid to neutralize gastric acid before taking this product orally, or give acid inhibitors intravenously at the same time; 3. This product can also be dissolved in phosphate buffer (pH 7.6) or cold milk. If a latex solution is prepared with gum arabic, gelatin, fructose gum, honey, etc., the hemostatic effect of thrombin can be improved, and the dosage of this product can be appropriately reduced.
Specifications	500 units
Manufacturer	Lanzhou Institute of Biological Products Co., Ltd.

Thrombin lyophilized powder
Ingredients	This product is a sterile lyophilized product of prothrombin extracted from bovine or pig blood and activated to obtain thrombin. The potency of each 1mg should not be less than 10 units. The thrombin content should be more than 80% of the labeled amount.
Indication	1. This product will react with acid, alkali and heavy metals and reduce its efficacy; 2. To improve the hemostatic effect of upper gastrointestinal bleeding, it is advisable to take a certain amount of antacid to neutralize gastric acid before taking this product orally, or give acid inhibitors intravenously at the same time; 3. This product can also be dissolved in phosphate buffer (pH 7.6) or cold milk. If a latex solution is prepared with gum arabic, gelatin, fructose gum, honey, etc., the hemostatic effect of thrombin can be improved, and the dosage of this product can be appropriately reduced.
Specifications	200 units

See More Related Drugs

Registered Holders

VASCULAR SOLUTIONS, INC.

European Union
Active
MP BIOMEDICALS NEW ZEALAND LIMITED

European Union
Active
BAYER CORP.

Brazil
Inactive

Recommended Suppliers of Thrombin

Greenbo Biochem Contact Supplier

CN
5 YRS

Business licensed Certified factory

Manufactory Supplier of Active Pharm Ingredients,Chemical Catalyst,Pharmaceutical Intermediates,Flavors and Fragrances,Agrochemicals,Chemical Pesticides,Organic Intermediates,OLED Intermediates

Thrombin 99% powder Professional Producer Greenbo

CAS No.: 9002-04-4

Grade: Pharmaceutical Grade

Content: 99%

Inquiry
Shaanxi Dideu Medichem Co. Ltd Contact Supplier

CN
5 YRS

Business licensed Certified factory

Manufactory Supplier of Chemical Pesticides,Food Additives,Agrochemicals,Active Pharm Ingredients,Flavors and Fragrances,Chemical Catalyst,Chemical Materials,Chem&Pharm Intermediates,Organic Intermediates,Feed Additive

Thrombin 99% Dideu-9002044 Dideu

CAS No.: 9002-04-4

Grade: Industrial Grade

Content: 99%

Inquiry
Creative Enzymes Contact Supplier

US
6 YRS

Business licensed Certified factory

Manufactory Supplier of Coenzymes,Inhibito,Enzymes,Zymogens,Substrates

Thrombin from Human, Recombinant

CAS No.: 9002-04-4

Content: 95%

Inquiry
Henan Lihao Chem Plant Limited Contact Supplier

CN
5 YRS

Business licensed

Trader Supplier of PVC resin,pvc paste resin,melamine

Thrombin 99% white powder kanbei

CAS No.: 9002-04-4

Grade: Industrial Grade

Content: 99%

Inquiry

Learn More Other Chemicals

Isocitrate dehydrogenase (NADP)

9028-48-2
Phosphate acetyltransferase

9029-91-8
β-Acetylhexosaminidinase

9012-33-3
Nicotinamide hypoxanthine dinucleotide phosphate reduced tetrasodium salt Formula

42934-87-2
Peptidase Formula

9031-96-3
L-Ornithine, N5-(aminocarbonyl)-, ethyl ester, monohydrochloride Formula

93964-75-1
HEPPSO Sodium salt N-[2-HYDROXYETHYL]P... Structure

89648-37-3
GLP-1 Structure

87805-34-3
What is [[(2S,3S,5R)-3-amino-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate

90053-18-2
What is Inulinase

9025-67-6

Hot Searches: n2o5 name; potassium sulfide chemical formula; sodium pentathol; potassium sulfide formula; acetanilide structure; boric acid acid

Guide of Thrombin

Description
Basic Attributes
Characteristics
Safety Information
Use and Manufacturing
Drug Function and Efficacy
Related Drugs
Registered Holders
Recommended Suppliers
Learn More Other Chemicals

Thrombin

SDS

Request for Quotation



Feedback & Suggestions

Send Message

Thank you for your feedback. If you require further assistance, please contact us by email at info@echemi.com or call us at +86-532-55729510.