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Brilliant Blue R

Brilliant Blue R structure

CAS No:
6104-59-2
Formula:
C45H45N3O7S2.Na
Chemical Name:
Brilliant Blue R
Synonyms:
Benzenemethanaminium,N-[4-[[4-[(4-ethoxyphenyl)amino]phenyl][4-[ethyl[(3-sulfophenyl)methyl]amino]phenyl]methylene]-2,5-cyclohexadien-1-ylidene]-N-ethyl-3-sulfo-,inner salt,sodium salt (1:1);C.I. Acid Blue 83;C.I. Acid Blue 83,monosodium salt;Benzenemethanaminium,N-[4-[[4-[(4-ethoxyphenyl)amino]phenyl][4-[ethyl[(3-sulfophenyl)methyl]amino]phenyl]methylene]-2,5-cyclohexadien-1-ylidene]-N-ethyl-3-sulfo-,hydroxide,inner salt,monosodium salt;Benzenemethanaminium,N-[4-[[4-[(4-ethoxyphenyl)amino]phenyl][4-[ethyl[(3-sulfophenyl)methyl]amino]phenyl]methylene]-2,5-cyclohexadien-1-ylidene]-N-ethyl-3-sulfo-,inner salt,monosodium salt;C.I. 42660;Acid Brilliant Cyanine 6B;Acidine Sky Blue R;Amacid Brilliant Cyanine 6B;Benzyl Cyanine 6B;Brilliant Acid Blue 6B;Brilliant Acid Cyanine 6B;Brilliant Indocyanine 6BA-CF;Coomassie Brilliant Blue R;Coriacide Blue 2R;Eriosin Brilliant Cyanine 6B;Fenazo Blue XXFB;Folan Blue 6B;Impero Azure 6B;Optanol Cyanine 6B;Polycor Blue R;Sapphire EMB;Sumitomo Brilliant Indocyanine 6B;Supranol Cyanine 6B;Xylene Brilliant Cyanine 6B;Acid Blue 83;Acid Brilliant Blue;Solar Cyanine 6B;Coomassie Blue R 250;Coomassie Blue R;Brilliant Indocyanine 6B;Brilliant Blue R;Serva Blue R 250;Kayanol Cyanine 6B;Acid bright blue;Chuganol Blue 6B;Acid Brilliant Blue 6B;Brilliant Blue R 250;Coomassie Brilliant Blue R 250;Brilliant Cyanine 6B;Weak Acid Brilliant Blue 6B;Kenacid Blue R;12698-46-3;86697-48-5;108066-47-3;1799495-73-0;2287196-89-6
Categories:
Dyes and Pigments > Dye

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Description

dark bordeaux to dark purplish-brown cryst. powder

Brilliant Blue R Basic Attributes

Molecular Weight：

825.97

Exact Mass：

825.251831

EC Number：

228-060-5

UNII：

TKL1K0PT9A

HScode：

32041200

Characteristics

PSA：

158.67000

XLogP3：

10.49970

Appearance：

Red Brown Powder/Solid

Melting Point：

174-180 °C

Flash Point：

14 °C

Water Solubility：

soluble in water.H2O: soluble 10mg/mL

Storage Conditions：

Store at RT.

Safety Information

Hazard category：

Transport codes：

UN12303/PG2

wgk germany：

hazard class code：

11-23/24/25-36/38-39/23/24/25-36/37/38-20/21/22-10

safety instructions：

22-24/25-45-36/37/39-26-36-36/37-16

Marks：

F,T,Xn,Xi

Stability/Shelf Life：

Stable under normal temperatures and pressures.

Precautionary statement(s)：

P305 + P351 + P338

Hazard Statements：

H226-H315-H319

GHS Classification：

|Warning|H302 (14.55%): Harmful if swallowed [Warning Acute toxicity, oral]|P264, P270, P273, P280, P301+P312, P305+P351+P338, P330, P337+P313, and P501|Aggregated GHS information provided by 83 companies from 5 notifications to the ECHA C&L Inventory. Each notification may be associated with multiple companies.

Drug Information

MeSH Entry Terms：

Coomassie blue R 250

Brilliant Blue R Use and Manufacturing

Methods of Manufacturing

Using p-chlorobenzaldehyde, 1-(N-ethylaniline) m-toluenesulfonic acid and p-phenetidine as raw materials, first p-chlorobenzaldehyde and 1-(N-ethylaniline) Condensate with m-toluenesulfonic acid, then oxidize the condensation product with potassium dichromate-oxalic acid, and finally condense with p-aminophenyl ether to obtain the product. After salting out, filtering, drying and crushing, the finished product is obtained. . After beating 1-(N-ethylaniline) m-toluenesulfonic acid with water, adjust the pH value to about 3, add p-chlorobenzaldehyde, and raise the temperature to 95-98°C to react to the end point. After distilling off excess p-chlorobenzaldehyde, dilute with water, lower the temperature, and add potassium dichromate, sulfuric acid and oxalic acid below 10°C for oxidation. At the end of the reaction, sulfuric acid was added at 45°C to precipitate a paste, which was dried in vacuum at 95°C. P-ethoxyaniline is heated to 70°C, then the above product is added, the temperature is raised to 100°C, and the reaction is performed for 3-6h to the end. Dilute with diluted hydrochloric acid at 70°C, filter, wash with hot water, and dry to obtain the product.

Uses

It is used for dyeing wool, silk, nylon and other fabrics, and also for dyeing biochemical research on leather and feathers. Used to detect protein bands after electrophoresis. It is 5-10 times more sensitive than Naphthol Blue Black B and Bromophenol (can detect 0.5μg/cm2 protein on the gel matrix). Triphenylmethane dye used for protein analysis in SDS-PAGE can also be widely used in Bradford protein quantitative reaction.

Manufacturing Info：

Benzenemethanaminium, N-[4-[[4-[(4-ethoxyphenyl)amino]phenyl][4-[ethyl[(3-sulfophenyl)methyl]amino]phenyl]methylene]-2,5-cyclohexadien-1-ylidene]-N-ethyl-3-sulfo-, inner salt, sodium salt (1:1): ACTIVE

Computed Properties

Molecular Weight:826.0
Hydrogen Bond Donor Count:1
Hydrogen Bond Acceptor Count:9
Rotatable Bond Count:13
Exact Mass:825.25183738
Monoisotopic Mass:825.25183738
Topological Polar Surface Area:159
Heavy Atom Count:58
Complexity:1500
Covalently-Bonded Unit Count:2
Compound Is Canonicalized:Yes

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