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Acid Blue 83

Acid Blue 83 structure

CAS No:
6104-59-2
Formula:
C45H46N3NaO7S2
Chemical Name:
Acid Blue 83
Synonyms:
SERVA BLUE R;SERVA BLUE R STAINING SOLUTION;SOLAR CYANINE 6B;R STANDS FOR ''REDDISH'';PAGE BLUE 83;Coomassie brilliant R250;Benzenemethanaminium, N-[4-[[4-[(4-ethoxyphenyl) amino]phenyl][4-[ethyl[(3-sulfophenyl)methyl]amino ]phenyl]methylene]-2,5-cyclohexadien-1-ylidene]- N-ethyl-3-sulfo-, hydroxide, inner salt, monosodium salt;Brilliant Blue 6B
Categories:
Dyes and Pigments > Dye

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Description

dark bordeaux to dark purplish-brown cryst. powder

Acid Blue 83 Basic Attributes

Molecular Weight：

827.99

Exact Mass：

825.251831

BRN：

5718025

EC Number：

228-060-5

UNII：

TKL1K0PT9A

TSCA：

TSCA listed

Color/Form：

Red Brown

HScode：

32041200

Characteristics

λmax：

589.2 nm in ethanol

PSA：

158.67000

XLogP3：

10.49970

Appearance：

Red Brown Powder/Solid

Melting Point：

174-180 °C

Flash Point：

14 °C

Water Solubility：

Soluble in water.

Storage Conditions：

Store at RT.

Vapor Density：

28.5 (vs air)

PH：

6.2 (25°C, 10g/L in H2O)

Critical Temperature & Pressure：

room temp

Safety Information

Hazard category：

Transport codes：

UN12303/PG2

wgk germany：

Marks：

F,T,Xn,Xi

Storage features：

Room temperature

Stability/Shelf Life：

Stable under normal temperatures and pressures.

Precautionary statement(s)：

P305 + P351 + P338

GHS Classification：

|Warning|H302 (14.55%): Harmful if swallowed [Warning Acute toxicity, oral]|P264, P270, P273, P280, P301+P312, P305+P351+P338, P330, P337+P313, and P501|Aggregated GHS information provided by 83 companies from 5 notifications to the ECHA C&L Inventory. Each notification may be associated with multiple companies.

Drug Information

MeSH Entry Terms：

Coomassie blue R 250

Acid Blue 83 Use and Manufacturing

Methods of Manufacturing

Using p-chlorobenzaldehyde, 1-(N-ethylaniline) m-toluenesulfonic acid and p-phenetidine as raw materials, first p-chlorobenzaldehyde and 1-(N-ethylaniline) Condensate with m-toluenesulfonic acid, then oxidize the condensation product with potassium dichromate-oxalic acid, and finally condense with p-aminophenyl ether to obtain the product. After salting out, filtering, drying and crushing, the finished product is obtained. . After beating 1-(N-ethylaniline) m-toluenesulfonic acid with water, adjust the pH value to about 3, add p-chlorobenzaldehyde, and raise the temperature to 95-98°C to react to the end point. After distilling off excess p-chlorobenzaldehyde, dilute with water, lower the temperature, and add potassium dichromate, sulfuric acid and oxalic acid below 10°C for oxidation. At the end of the reaction, sulfuric acid was added at 45°C to precipitate a paste, which was dried in vacuum at 95°C. P-ethoxyaniline is heated to 70°C, then the above product is added, the temperature is raised to 100°C, and the reaction is performed for 3-6h to the end. Dilute with diluted hydrochloric acid at 70°C, filter, wash with hot water, and dry to obtain the product.

Uses

It is used for dyeing wool, silk, nylon and other fabrics, and also for dyeing biochemical research on leather and feathers. Used to detect protein bands after electrophoresis. It is 5-10 times more sensitive than Naphthol Blue Black B and Bromophenol (can detect 0.5μg/cm2 protein on the gel matrix). Triphenylmethane dye used for protein analysis in SDS-PAGE can also be widely used in Bradford protein quantitative reaction.

Acid Blue 83 is a dye commonly used in laboratories or quantify proteins.

Brilliant Blue R is used for detection of protein bands following electrophoresis. It is a sensitive stain for protein detection in polyacrylamide gels, typically giving blue bands on a clear background with a sensitivity of 50-100 ng/band. It may be combined with other stains, such as silver stain, to distinguish different types of proteins. It displays metachromasia by appearing pink-red, rather than blue, when binding certain proteins, such as collagen and histone H1.

Manufacturing Info：

Benzenemethanaminium, N-[4-[[4-[(4-ethoxyphenyl)amino]phenyl][4-[ethyl[(3-sulfophenyl)methyl]amino]phenyl]methylene]-2,5-cyclohexadien-1-ylidene]-N-ethyl-3-sulfo-, inner salt, sodium salt (1:1): ACTIVE

Computed Properties

Molecular Weight:826.0
Hydrogen Bond Donor Count:1
Hydrogen Bond Acceptor Count:9
Rotatable Bond Count:13
Exact Mass:825.25183738
Monoisotopic Mass:825.25183738
Topological Polar Surface Area:159
Heavy Atom Count:58
Complexity:1500
Covalently-Bonded Unit Count:2
Compound Is Canonicalized:Yes

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