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Evan's Blue

Evan's Blue structure

CAS No:
314-13-6
Formula:
C34H28N6O14S4.4Na
Chemical Name:
Evan's Blue
Synonyms:
1,3-Naphthalenedisulfonic acid,6,6′-[(3,3′-dimethyl[1,1′-biphenyl]-4,4′-diyl)bis(2,1-diazenediyl)]bis[4-amino-5-hydroxy-,sodium salt (1:4);Azovan Blue;C.I. Direct Blue 53,tetrasodium salt;1,3-Naphthalenedisulfonic acid,6,6′-[(3,3′-dimethyl[1,1′-biphenyl]-4,4′-diyl)bis(azo)]bis[4-amino-5-hydroxy-,tetrasodium salt;T 1824;C.I. 23860;4,4′-Bis[7-(1-amino-8-hydroxy-2,4-disulfo)naphthylazo]-3,3′-bitolyl tetrasodium salt;Diazol Pure Blue BF;Evans Blue;C.I. Direct Blue 53;Direct Blue 53;Evan's Blue;179472-53-8
Categories:
Active Pharmaceutical Ingredients > Diagnostic Agents

Description

Evans Blue is a potent inhibitor of L-glutamate uptake via the membrane bound excitatory amino acid transporter (EAAT). Able to antagonize AMPA and kainate receptor mediated currents (IC50 values are 220 and 150 nM, respectively). IC50 value: 220/150 nMTarget: L-glutamate uptake receptorEvans blue is a biological dye, for detection of cell survival. Evans Blue can not penetrate healthy cell membrane, but can penetrate into death cell. Binds with high affinity to serum albumin.

Direct blue 53 appears as blue crystals with a greenish-bronze luster or a black powder. (NTP, 1992)

Direct blue 53 appears as blue crystals with a greenish-bronze luster or a black powder. (NTP, 1992)|Evans blue is an organic sodium salt that is the tetrasodium salt of 6,6'-{(3,3'-dimethyl[1,1'-biphenyl]-4,4'-diyl)bis[diazene-2,1-diyl]}bis(4-amino-5-hydroxynaphthalene-1,3-disulfonate). It is sometimes used as a counterstain, especially in fluorescent methods to suppress background autofluorescence. It has a role as a histological dye, a fluorochrome, a teratogenic agent and a sodium channel blocker. It contains an Evans blue(4-).|An azo dye used in blood volume and cardiac output measurement by the dye dilution method. It is very soluble, strongly bound to plasma albumin, and disappears very slowly.

Evan's Blue Basic Attributes

Molecular Weight：

960.81

Exact Mass：

959.982422

EC Number：

206-242-5

UNII：

45PG892GO1

DSSTox ID：

DTXSID9022815

HScode：

32129000

Characteristics

PSA：

404.26000

XLogP3：

10.78520

Appearance：

Dark Brown Powder/Solid

Water Solubility：

280 g/L (20 ºC)

Storage Conditions：

Store at RT.

Toxicity：

Abdominal cavity-mouse LDL0: 200 mg/kg

Flammability characteristics：

Combustible; burning produces toxic nitrogen oxides, sulfur oxides and sodium oxide fumes

Air and Water Reactions：

Azo dyes can be explosive when suspended in air at specific concentrations. Soluble in water.

Reactive Group：

Azo, Diazo, Azido, Hydrazine, and Azide Compounds

Reactivity Alerts：

Explosive

Reactivity Profile：

DIRECT BLUE 53 is an azo compound. Azo, diazo, azido compounds can detonate. This applies in particular to organic azides that have been sensitized by the addition of metal salts or strong acids. Toxic gases are formed by mixing materials of this class with acids, aldehydes, amides, carbamates, cyanides, inorganic fluorides, halogenated organics, isocyanates, ketones, metals, nitrides, peroxides, phenols, epoxides, acyl halides, and strong oxidizing or reducing agents. Flammable gases are formed by mixing materials in this group with alkali metals. Explosive combination can occur with strong oxidizing agents, metal salts, peroxides, and sulfides. This compound can react with strong reducing and oxidizing agents. (NTP, 1992)

Safety Information

Transport codes：

NONH for all modes of transport

wgk germany：

hazard class code：

45-63-11-61-20/21/22

safety instructions：

53-45-36/37-16-36/37/39

RTECS Number：

QJ6440000

Marks：

T,F

Storage features：

Treasury is ventilated, low temperature and dry; stored separately from food materials

Stability/Shelf Life：

Stable. Incompatible with strong reducing agents, strong oxidizing agents.

Precautionary statement(s)：

P201-P308 + P313

Hazard Statements：

H350

Fire Hazards：

Flash point data for this compound are unavailable; however, it is probably combustible. (NTP, 1992)

GHS Classification：

|Danger|H341: Suspected of causing genetic defects [Warning Germ cell mutagenicity]|P201, P202, P281, P308+P313, P405, and P501

Fire Fighting：

Fires involving this compound can be controlled using a dry chemical, carbon dioxide, or Halon extinguisher. (NTP, 1992)

Nonfire Spill Response：

SMALL SPILLS AND LEAKAGE: If you spill this chemical, you should dampen the solid spill material with water, then transfer the dampened material to a suitable container. Use absorbent paper dampened with water to pick up any remaining material. Seal your contaminated clothing and the absorbent paper in a vapor-tight plastic bag for eventual disposal. Wash all contaminated surfaces with a soap and water solution. Do not reenter the contaminated area until the Safety Officer (or other responsible person) has verified that the area has been properly cleaned. STORAGE PRECAUTIONS: You should store this chemical under refrigerated temperatures, and keep it away from oxidizing materials. (NTP, 1992)

Personal Protective Equipment：

RECOMMENDED RESPIRATOR: Where the neat test chemical is weighed and diluted, wear a NIOSH-approved half face respirator equipped with an organic vapor/acid gas cartridge (specific for organic vapors, HCl, acid gas and SO2) with a dust/mist filter. (NTP, 1992)

Toxicity

Toxicity class：

highly

Drug Information

MeSH：

Chemicals and substances that impart color including soluble dyes and insoluble pigments. They are used in INKS; PAINTS; and as INDICATORS AND REAGENTS. (See all compounds classified as Coloring Agents.)

Health Hazards：

SYMPTOMS: This compound may cause irritation. ACUTE/CHRONIC HAZARDS: This compound may cause irritation. When heated to decomposition it emits toxic fumes. (NTP, 1992)

First Aid：

EYES: First check the victim for contact lenses and remove if present. Flush victim's eyes with water or normal saline solution for 20 to 30 minutes while simultaneously calling a hospital or poison control center. Do not put any ointments, oils, or medication in the victim's eyes without specific instructions from a physician. IMMEDIATELY transport the victim after flushing eyes to a hospital even if no symptoms (such as redness or irritation) develop. SKIN: IMMEDIATELY flood affected skin with water while removing and isolating all contaminated clothing. Gently wash all affected skin areas thoroughly with soap and water. If symptoms such as redness or irritation develop, IMMEDIATELY call a physician and be prepared to transport the victim to a hospital for treatment. INHALATION: IMMEDIATELY leave the contaminated area; take deep breaths of fresh air. If symptoms (such as wheezing, coughing, shortness of breath, or burning in the mouth, throat, or chest) develop, call a physician and be prepared to transport the victim to a hospital. Provide proper respiratory protection to rescuers entering an unknown atmosphere. Whenever possible, Self-Contained Breathing Apparatus (SCBA) should be used; if not available, use a level of protection greater than or equal to that advised under Protective Clothing. INGESTION: DO NOT INDUCE VOMITING. If the victim is conscious and not convulsing, give 1 or 2 glasses of water to dilute the chemical and IMMEDIATELY call a hospital or poison control center. Be prepared to transport the victim to a hospital if advised by a physician. If the victim is convulsing or unconscious, do not give anything by mouth, ensure that the victim's airway is open and lay the victim on his/her side with the head lower than the body. DO NOT INDUCE VOMITING. IMMEDIATELY transport the victim to a hospital. (NTP, 1992)

MeSH Entry Terms：

Azovan Blue

Evan's Blue Use and Manufacturing

Uses

antineoplastic This product is a diagnostic drug used to determine the amount of hemolysis.

Manufacturing Info：

1,3-Naphthalenedisulfonic acid, 6,6'-[(3,3'-dimethyl[1,1'-biphenyl]-4,4'-diyl)bis(2,1-diazenediyl)]bis[4-amino-5-hydroxy-, sodium salt (1:4): ACTIVE

Use Classification：

Human Drugs -> FDA Approved Drug Products with Therapeutic Equivalence Evaluations (Orange Book) -> Active Ingredients

Computed Properties

Molecular Weight:960.8
Hydrogen Bond Donor Count:4
Hydrogen Bond Acceptor Count:20
Rotatable Bond Count:5
Exact Mass:959.9824113
Monoisotopic Mass:959.9824113
Topological Polar Surface Area:404
Heavy Atom Count:62
Complexity:1790
Covalently-Bonded Unit Count:5
Compound Is Canonicalized:Yes

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CAS No.: 314-13-6

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EVANS BLUE 314-13-6

CAS No.: 314-13-6

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